The sophisticated parasitic tactic of sedentary endoparasitic nematodes seems to involve the simultaneous alteration of the expression of multitude of its effector genes in order to hijack the plant metabolic and developmental pathway. In concordance with this hypothesis, we have targeted some candidate effector genes of Meloidogyne incognita to understand the possible interaction among those effectors for successful infection of the host plant. In vitro RNAi strategy was used to knock down M. incognita-specific pioneer effector genes, such as msp-18, msp-20, msp-24, msp-33 and msp-16 (known to interact with plant transcription factor), to investigate their possible effect on the expression of key cell wall-degrading enzymes (CWDE) and vice versa. Supported by the phenotypic data, intriguingly our study revealed that induced suppression of these pioneer genes cause transcriptional alteration of CWDE genes in M. incognita. This remarkable finding may provide some useful links for future research on nematode effector interaction.
Characterisation by RNAi of pioneer genes expressed in the dorsal pharyngeal gland cell of Heterodera glycines and the effects of combinatorial RNAi.
International Journal for Parasitology381589-1597.
Plant-mediated RNA interference of effector gene Mc16D10L confers resistance against Meloidogyne chitwoodi in diverse genetic backgrounds of potato and reduces pathogenicity of nematode offspring.
Comparison of host recognition, invasion, development and reproduction of Meloidogyne graminicola and M. incognita on rice and tomato.
Journal of Nematology13509-520.
The interaction of the novel 30C02 cyst nematode effector protein with a plant beta-1,3 endoglucanase may suppress host defence to promote parasitism.
Journal of Experimental Botany633683-3695.
Cellulose binding protein from the parasitic nematode Heterodera schachtii interacts with Arabidopsis pectin methylesterase: cooperative cell wall modification during parasitism.
Arabidopsis spermidine synthase is targeted by an effector protein of the cyst nematode Heterodera schachtii.
Extraction of free-living stages from soil. In:
Laboratory methods for work with plant and soil nematodes. London, UKHer Majesty’s Stationery Office, Ministry of Agriculture, Fisheries & Food pp.
A profile of putative parasitism genes expressed in the esophageal gland cells of the root-knot nematode Meloidogyne incognita.
Molecular Plant-Microbe Interactions16376-381.
Use of solid-phase subtractive hybridization for the identification of parasitism gene candidates from the root-knot nematode Meloidogyne incognita.
Molecular Plant Pathology5217-222.
Developmental expression and molecular analysis of two Meloidogyne incognita pectate lyase genes.
International Journal for Parasitology35685-692.
Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene.
Proceedings of the National Academy of Sciencesof the United States of America10314302-14306.
JaouannetM.Perfus-BarbeochL.DeleuryE.MaglianoM.EnglerG.VieiraP.DanchinE.G.J.Da RochaM.CoquillardP.AbadP. (2012).
A root knot nematode-secreted protein is injected into giant cells and targeted to the nuclei.
Characterization of a new β-1,4-endoglucanase gene from the root-knot nematode Meloidogyne incognita and evolutionary scheme for phytonematode family 5 glycosyl hydrolases.
The novel cyst nematode effector protein 19C07 interacts with the Arabidopsis auxin influx transporter LAX3 to control feeding site development.
Small dsRNAs induce transcriptional activation in human cells.
Proceedings of the National Academy of Sciences of the United States of America10317337-17342.
Mitreva-DautovaM.RozeE.OvermarsH.De GraaffL.SchotsA.HelderJ.GoverseA.BakkerJ.SmantG. (2006).
A symbiont-independent endo-1,4-β-xylanase from the plant-parasitic nematode Meloidogyne incognita.
Molecular Plant-Microbe Interactions19521-529.
RossoM.-N.FaveryB.PiotteC.ArthaudL.De BoerJ.M.HusseyR.S.BakkerJ.BaumT.J.AbadP. (1999).
Isolation of a cDNA encoding a β-1,4-endoglucanase in the root-knot nematode Meloidogyne incognita and expression analysis during plant parasitism.
Molecular Plant-Microbe Interactions12585-591.
SmantG.StokkermansJ.P.W.G.YanY.De BoerJ.M.BaumT.J.WangX.HusseyR.S.GommersF.J.HenrissatB.DavisE.L. (1998).
Endogenous cellulases in animals: isolation of β-1,4-endoglucanase genes from two species of plant-parasitic cyst nematodes.
Proceedings of the National Academy of Sciences of the United States of America954906-4911.