Most studies on distribution of Phasmarhabditis spp. in slugs use dissection of individual slugs, which is time-consuming. Here we use a technique modified from that used to collect Pristionchus spp. nematodes from their beetle hosts. Slugs are decapitated and cadavers incubated for 1 week prior to examining for presence of adult nematodes. We compared the new technique with traditional dissection using field-collected untreated slugs, and slugs infected with Phasmarhabditis hermaphrodita in the laboratory. There was no difference in the efficacy of the two techniques. We also used the new technique to study prevalence of P. hermaphrodita at 22 New Zealand sites. We found P. hermaphrodita present at three sites and P. californica at two other sites suggesting Phasmarhabditis spp. are relatively common in New Zealand.
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First record for the slug parasitic nematode, Phasmarhabditis hermaphrodita (Schneider) in Egypt.
Archives of Phytopathology and Plant Protection44340-345.
Description and systematic affinity of Alaninema ngata n. sp. (Alaninematidae: Panagrolaimorpha) parasitising leaf-veined slugs (Athoracophoridae: Pulmonata) in New Zealand.
A phylogeny and molecular barcodes for Caenorhabditis, with numerous new species from rotting fruits.
BMC Evolutionary Biology11339.
Dispersal patterns and behaviour of the nematode Phasmarhabditis hermaphrodita in mineral soils and organic media.
Soil Biology & Biochemistry411483-1490.
The effect of different growing substrates on the development and quality of Phasmarhabditis hermaphrodita (Nematoda: Rhabditidae).
Biocontrol Science and Technology241026-1038.
Diversity and distribution of nematodes associated with terrestrial slugs in the Western Cape Province of South Africa.
Journal of Helminthology86215-221.
Tandingan De LeyI.T.HolovachovO.McDonnellR.J.BertW.PaineT.D.De LeyP. (2016).
Description of Phasmarhabditis californica n. sp. and first report of P. papillosa (Nematoda: Rhabditidae) from invasive slugs in the USA.
Mass cultivation and storage of the rhabditid nematode Phasmarhabditis hermaphrodita, a biocontrol agent for slugs.
Biocontrol Science and Technology3513-521.
Laboratory tests of the potential of entomopathogenic nematodes for the control of field slugs (Deroceras reticulatum).
Journal of Invertebrate Pathology64182-187.
Invasive slug pests and their parasites – temperature responses and potential implications of climate change.
Biology and Fertility of Soils51739-748.