Aphelenchoides iranicus n. sp. (Nematoda: Aphelenchoididae) from West Azerbaijan province, Iran

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Aphelenchoides iranicus n. sp. is described and illustrated from bark samples of an oak tree (Quercus sp.) in West Azerbaijan province, north-western Iran. The new species is characterised by body length of 350 (330-383) μm in females and 343 (323-370) μm in males, lip region set off from body contour, lateral fields with three lines, stylet 8 (7-9) μm long with small basal swellings, excretory pore 59 (56-62) μm from anterior end, hemizonid invisible, pharyngeal glands overlapping intestine dorsally and extending for 48 (41-54) μm, female tail subcylindrical with many tiny finger-like appendages at the tip, and male with aphelenchoid type spicules 17.6 (17-18) μm long. The new species comes close to nine known species of the genus namely: A. brevicaudatus, A. cibolensis, A. conimucronatus, A. editocaputis, A. montanus, A. pusillus, A. rarus, A. sphaerocephalus and A. vaughani, but differs from them by the number of incisures in the lateral field, shape of tail mucron, stylet length, size of spicules and length of post-vulval uterine sac. Additionally, this species is characterised molecularly and phylogenetically by sequences of the D2-D3 expansion segments of the 28S and partial 18S rRNA genes that are unique in relation to the sequences deposited in GenBank.

Aphelenchoides iranicus n. sp. (Nematoda: Aphelenchoididae) from West Azerbaijan province, Iran

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References

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Figures

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    Line drawing of Aphelenchoides iranicus n. sp. A: Female entire body; B: Male entire body; C: Stylet; D: Female anterior head; E: Metacorpus region; F: Anterior body; G: Vulval region and lateral field; H: Male posterior body; I: Female posterior body. (Scale bars: A-C, E, F = 5 μm; D, G-I = 10 μm.)

  • View in gallery

    Light micrographs of Aphelenchoides iranicus n. sp. A: Female anterior body; B: Female head region; C: Cross section of female showing lateral field; D: Metacorpus region showing excretory pore (arrowhead); E: Vulval region showing post-uterine sac (PUS, arrowhead); F, G: Female tails; H: Male posterior body showing P1-P3 papillal arrangement (arrowheads, ventral view); I, J: Male posterior body showing spicules and papillal arrangement. (Scale bars: A, B, D-J = 10 μm; C = 5 μm.)

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    Scanning electron microscope photographs of Aphelenchoides iranicus n. sp. A: Lateral view of female head; B: En face view of female; C: Lateral view of anterior region showing detail and beginning of lateral fields; D: Anterior region showing excretory pore arrowed; E: Vulval region; F: Lateral lines in vulval region; G: Female posterior body; I: Tail region showing anus; H, J, K: Tail terminus. (Scale bars: A = 1 μm; B, H, J = 0.5 μm; C-G, I, K = 2 μm.)

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    The 50% majority rule consensus trees from Bayesian analysis generated from the D2-D3 of 28S rRNA gene dataset of Aphelenchoididae with the TVM + I + G model. Posterior probabilities ⩾70% are given for appropriate clades (− = posterior probability <70%). Newly obtained sequence is in bold letters and the scale bar indicates the expected changes per site. This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

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    The 50% majority rule consensus trees from Bayesian analysis generated from the partial 18S rRNA gene dataset of Aphelenchoididae with the TIM2 + I + G model. Posterior probabilities ⩾70% are given for appropriate clades (− = posterior probability <70%). Newly obtained sequence is in bold letters and the scale bar indicates the expected changes per site. This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

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