Molecular and morphological characterisation of Ditylenchus persicus n. sp. (Nematoda: Anguinidae) from Kermanshah province, western Iran

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A new species of the genus Ditylenchus, D. persicus n. sp., was collected around the roots of grapevine and is described and illustrated herein based on morphological and molecular studies. The new species is characterised by a body length of 783 (635-928) μm and 689 (670-715) μm in female and male, respectively, delicate stylet 6.2 (5.0-7.0) μm long, six lines in the lateral field, median bulb of pharynx well developed, muscular with crescentic valve, basal pharyngeal bulb pyriform with 10 (8-13) μm long stem extending into intestine, post-vulval uterine sac relatively short, 16 (14-18) μm long, female and male tail elongate-conoid with finely rounded terminus, and bursa covering 65-80% of male tail length. Morphologically, D. persicus n. sp. appears closer to five known species of the genus, namely: D. arachis, D. caudatus, D. clarus, D. myceliophagus and D. nanus. The results of phylogenetic analyses based on sequences of D2-D3 expansion region of 28S rRNA, ITS and partial 18S rRNA genes confirmed the close molecular relationship between D. persicus n. sp. and other Ditylenchus species such as D. myceliophagus, D. africanus, D. arachis, D. destructor and D. halictus.

Nematology

International Journal of Fundamental and Applied Nematological Research

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Figures

  • Line drawing of Ditylenchus persicus n. sp. A: Female entire body; B: Male entire body; C: Female lip region; D: Female pharyngeal region; E: Detail of basal bulb; F: Lateral field at mid-body; G: Female posterior body region; H: Male posterior region. (Scale bars = 30 μm.)

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  • Light micrographs of Ditylenchus persicus n. sp. A, B: Female anterior body region; C: Vulval region and post-vulval uterine sac (arrowhead); D: Lateral field at mid-body; E-G: Female pharyngeal region showing excretory pore (arrowheads) and basal pharyngeal bulb extension (arrows); H, I: Female tail region; J: Male tail region; K: Detail of spicules. (Scale bars = 10 μm.)

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  • Light micrographs of Ditylenchus persicus n. sp. A-C: Female pharyngeal region showing basal pharyngeal bulb extension (arrows); D: Female pharyngeal region showing excretory pore (arrowhead) and hemizonid (arrow). (Scale bars = 20 μm.)

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  • The 50% majority rule consensus trees from Bayesian analysis generated from the D2-D3 of 28S rRNA gene dataset of anguinid nematodes with the GTR + G model. Posterior probabilities ⩾ 70% are given for appropriate clades (– = posterior probability < 70%). Newly obtained sequence in bold letters. (Scale bar = expected changes per site.)

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  • The 50% majority rule consensus trees from Bayesian analysis generated from the ITS rRNA gene dataset of anguinid nematodes with the GTR + G model. Posterior probabilities ⩾ 70% are given for appropriate clades (– = posterior probability < 70%). Newly obtained sequence in bold letters. (Scale bar = expected changes per site.)

    View in gallery
  • The 50% majority rule consensus trees from Bayesian analysis generated from the partial 18S rRNA gene dataset of anguinid nematodes with the GTR + I + G model. Posterior probabilities ⩾ 70% are given for appropriate clades (– = posterior probability < 70%). Newly obtained sequence in bold letters. (Scale bar = expected changes per site.)

    View in gallery

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