Four new species and five morphospecies of Ficophagus Davies & Bartholomaeus, 2015 (Nematoda: Aphelenchoididae) from Ficus subgenus Urostigma sect. Americana (Moraceae) in Central America

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Four new species of Ficophagus were recovered from the sycones of Ficus spp. from the Subgenus Urostigma section Americana from Central America. The species are described here as Ficophagus bullenei sp. n., F. costaricanus sp. n., F. paraensis sp. n. and F. kayai sp. n. The new species are differentiated from other species of the genus by a combination of morphological characters including body length, position of the excretory pore, spicule shape, stylet form, length of the post-uterine sac, tail shape, sequence data of the ribosomal DNA 18S, 28S D2-D3 and mitochondrial DNA cytochrome oxidase subunit I, biogeographical range, and host wasp and Ficus species affiliations. In addition, five morphospecies are described from Ficus cahuitensis, F. citrifolia, F. dugandii, F. obtusifolia, and F. trigonata, respectively. The morphospecies are not formally named due to a lack of pertinent data/molecular sequences.

Four new species and five morphospecies of Ficophagus Davies & Bartholomaeus, 2015 (Nematoda: Aphelenchoididae) from Ficus subgenus Urostigma sect. Americana (Moraceae) in Central America

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References

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Figures

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    Ficophagus bullenei sp. n. in lateral view. A: Entire reproductive female; B: Entire infective female; C: Entire male; D: Male head; E: Male tail region (diagrammatic); F: Female tail region; G: Spermatheca, vulva and PUS; H: Spicules. (Scale bars: A-C = 50 μm; D, G = 25 μm; F = 10 μm; H = 5 μm.)

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    Ficophagus costaricanus sp. n. in lateral view. A: Entire reproductive female; B: Entire male; C: Lateral lines from males; D: Spermatheca, vulva and PUS; E: Anterior end of female; F: Female tail region (diagrammatic); G: Male tail region; H: Spicules. (Scale bars: A, B = 50 μm; C, H = 5 μm; D, E = 25 μm; G = 10 μm.)

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    Ficophagus kayai sp. n. in lateral view. A: Entire reproductive female; B: Entire male; C: Female tail region; D: Male head; E: Spermatheca, vulva and PUS; F: Male tail region (diagrammatic); G: Spicules. (Scale bars: A, B = 50 μm; C = 10 μm; D, E = 25 μm; G = 5 μm.)

  • View in gallery

    Ficophagus paraensis sp. n. in lateral view. A: Entire reproductive female; B: Entire male; C: Spermatheca, vulva and PUS; D: Anterior end of female; E: Male tail region; F: Spicules; G: Female tail region. (Scale bars: A, B = 50 μm; C, D = 25 μm; E, F = 5 μm; G = 10 μm.)

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    Ficophagus MSp 16 from Ficus cahuitensis in lateral view. A: Entire reproductive female; B: Entire male; C: Lateral lines; D: Anterior end of male; E: Spermatheca, vulva and PUS; F: Female tail region; G: Spicules; H: Male tail region. (Scale bars: A, B = 50 μm; C, G = 5 μm; D, E = 25 μm; F, H = 10 μm.)

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    Ficophagus MSp 17 from Ficus citrifolia in lateral view. A: Entire pre-reproductive female; B: Entire male; C: Spermatheca, vulva and PUS; D: Anterior end of female; E: Female tail region (diagrammatic); F: Male tail region; G: Spicules. (Scale bars: A, B = 50 μm; C, D = 25 μm; F = 10 μm; G = 5 μm.)

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    Ficophagus MSp 24 from Ficus dugandii in lateral view. A: Entire reproductive female; B: Entire male; C: Spermatheca, vulva and PUS; D: Anterior end of female; E: Female tail region (diagrammatic); F: Spicules; G: Male tail region. (Scale bars: A, B = 50 μm; C, D = 25 μm; F = 5 μm; G = 10 μm.)

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    Ficophagus MSp 27 from Ficus obtusifolia in lateral view. A: Entire reproductive female; B: Entire male; C: Lateral lines; D: Male tails; E: Spermatheca, vulva and PUS; F: Portion of pre-reproductive female showing pharyngeal glands and position of hemizonid; G: Anterior end of female; H: Spicules; I: Female tail region (diagrammatic). (Scale bars: A, B = 50 μm; C, H = 5 μm; D = 10 μm; E-G = 25 μm.)

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    Ficophagus MSp 31 from Ficus trigonata in lateral view. A: Entire female; B: Entire male; C: Entire infective female; D: Lateral lines (diagrammatic); E: Female tail region (diagrammatic); F: Anterior end of pre-reproductive female; G: Spermatheca, vulva and PUS; H: Spicule; I: Male tail region (diagrammatic). (Scale bars: A-C = 50 μm; F, G = 25 μm; H = 5 μm.)

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    Photomicrographs of heads of Ficophagus from figs from subgenus and subsection Urostigma Americana in lateral view. Arrowheads = excretory pore location. A: Ficophagus bullenei sp. n.; B: Ficophagus costaricanus sp. n.; C: Ficophagus kayai sp. n.; D: Ficophagus paraensis sp. n.; E: Ficophagus MSp 16 from Ficus cahuitensis; F: Ficophagus MSp 17 from Ficus citrifolia; G: Ficophagus MSp 24 from Ficus dugandii; H: Ficophagus MSp 27 from Ficus obtusifolia; I: Ficophagus MSp 31 from Ficus trigonata.

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    Photomicrographs of the spicules of new species of Ficophagus from figs from the Subgenus and subsection Urostigma Americana in lateral view. A: Ficophagus bullenei sp. n.; B: Ficophagus costaricanus sp. n.; C: Ficophagus kayai sp. n.; D: Ficophagus paraensis sp. n. (Scale bar = 10 μm.)

  • View in gallery

    Photomicrographs of spicules of Ficophagus morphospecies from figs from Subgenus and subsection Urostigma Americana in lateral view. A: Ficophagus MSp 16 from Ficus cahuitensis; B: Ficophagus MSp 17 from Ficus citrifolia; C: Ficophagus MSp 24 from Ficus dugandii; D: Ficophagus MSp 27 from Ficus obtusifolia; E: Ficophagus MSp 31 from Ficus trigonata. (Scale bar = 10 μm.)

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