Interspecific and intraspecific competition in the potato cyst nematodes Globodera pallida and G. rostochiensis

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The effects of interspecific competition between the potato cyst nematodes (PCN), Globodera pallida and G. rostochiensis, under variable population conditions were examined. PCN-susceptible potato cultivars were inoculated with single- and mixed-species populations of PCN. There was a significant difference in species multiplication between single- and mixed-species populations. Globodera rostochiensis multiplication in mixed-species populations was significantly lower than single-species populations. Density-dependent trials showed a significantly greater increase in multiplication of G. pallida at low inoculation densities relative to G. rostochiensis. These results indicate that G. pallida has a negative effect on G. rostochiensis populations and is the more successful species when competing. A staggered inoculation trial showed that the greater competitiveness of G. pallida was not wholly attributed to its later hatch. Globodera pallida outcompeted G. rostochiensis regardless of the timing of inoculation; however, G. pallida multiplication was lowest when plants were simultaneously inoculated with both species.


International Journal of Fundamental and Applied Nematological Research



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  • The effect of staggered inoculation on Globodera pallida and G. rostochiensis multiplication (Pf/Pi). Treatments were subject to inoculation intervals of 0 (simultaneous inoculation) or 7 days. Data were normalised using log10 transformation and analysed with a three-way ANOVA. Samples with a common letter are not significantly different (P>0.05) using the Tukey test; n = 10.

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  • The effect of inoculation density (proportion of Globodera pallida Pi:G. rostochiensis Pi) on the individual multiplication rate (Pf/Pi) of G. pallida (A) and G. rostochiensis (B). The initial inoculation densities levels were 0, 1310, 5240, 6550, 13 100, 19 650, 20 960, 24 890 and 26 200 second-stage juveniles (J2) pot−1 for G. pallida and 0, 1340, 5360, 6700, 13 400, 20 100, 21 440, 25 460 and 26 800 J2 pot−1 for G. rostochiensis. The data (n = 10) represent the multiplication (Pf/Pi) individual species and not the total population (i.e., non-cumulative Pi). The (Pf/Pi) of individual species was calculated as G. pallida Pf/G. pallida Pi and G. rostochiensis Pf/G. rostochiensis Pi.

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  • The effect of initial population density (% Pi) of Globodera pallida (black columns) and G. rostochiensis (grey columns) multiplication (Pf/Pi) on cv. Kerr’s Pink (A) and cv. Rooster (B). The data (n = 10) represent the relative multiplication of either species; Pf of G. pallida and G. rostochiensis as a function of the total Pi, i.e., cumulative Pi G. pallida and Pi G. rostochiensis. As such, the total Pi in each treatment is 26 800 second-stage juveniles pot−1. Data were analysed using three-way ANOVA after log10 transformation. An asterisk denotes a significant difference between species (P<0.05) using the Tukey test.

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  • The relationship between initial population density (Pi) and final population density (Pf) of Globodera pallida (■) and G. rostochiensis (▲) on cvs Kerr’s Pink, British Queen and Rooster. The data (n = 10) represent the Pf of G. pallida and G. rostochiensis as a function of the total Pi, i.e., cumulative Pi G. pallida and Pi G. rostochiensis. The total Pi in each treatment is 26 800 second-stage juveniles pot−1. Data represent quadratic and cubic regression analysis of Pf vs Pi nematode populations. Inocula were applied as cysts to achieve Pi values of 0-26 200 eggs pot−1 for G. pallida and 0-26 800 eggs pot−1 for G. rostochiensis.

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