Comparisons between two Antarctic nematodes: cultured Panagrolaimus sp. DAW1 and field-sourced Panagrolaimus davidi

in Nematology
Restricted Access
Get Access to Full Text
Rent on DeepDyve

Have an Access Token?



Enter your access token to activate and access content online.

Please login and go to your personal user account to enter your access token.



Help

Have Institutional Access?



Access content through your institution. Any other coaching guidance?



Connect

The Antarctic nematode Panagrolaimus davidi can survive intracellular freezing. Genetic studies indicate the culture strain (now designated as Panagrolaimus sp. DAW1) is a different species to the P. davidi of field origin. This paper reports further attempts both to isolate DAW1 from Antarctic soils and to culture P. davidi itself. Sequencing of the 18S rRNA gene of 151 individuals indicates that DAW1 is rare in the field, but characterising two new isolates shows that, nevertheless, it is present. Panagrolaimus davidi is common in the field, but cannot be cultured using the media tested here. These two species are difficult to distinguish morphologically, apart from the absence of males in DAW1 and its longer recurved tail. Whilst it is possible that DAW1 is an introduced species, the sites at Shackleton’s hut at Cape Royds are dominated by P. davidi, which is clearly an endemic species.

Comparisons between two Antarctic nematodes: cultured Panagrolaimus sp. DAW1 and field-sourced Panagrolaimus davidi

in Nematology

Sections

References

  • AliF.WhartonD.A. (2014). Intracellular freezing in the infective juveniles of Steinernema feltiae: an entomopathogenic nematode. PLoS ONE 9(4) e94179. DOI: 10.1371/journal.pone.0094179

  • BarrièreA.FélixM.-A. (2014). Isolation of C. elegans and related nematodes. In: The C. elegans Research Community (Ed.). WormBook available online at http://www.wormbook.org. DOI: 10.1895/wormbook.1.115.2

  • EdgarR.C. (2004). MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 321792-1797. DOI: 10.1093/nar/gkh340

  • FloydR.M.RogersA.D.LambsheadP.J.D.SmithC.R. (2005). Nematode-specific PCR primers for the 18S small subunit rRNA gene. Molecular Ecology Notes 5611-612. DOI: 10.1111/j.1471-8286.2005.01009.x

  • HallT. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 4195-98.

  • HooperD.J. (1986a). Extraction of free-living stages from soil. In: SoutheyJ.F. (Ed.). Laboratory methods for work with plant and soil nematodes. London, UKHMSO pp.  5-30.

  • HooperD.J. (1986b). Handling, fixing, staining and mounting nematodes. In: SoutheyJ.F. (Ed.). Laboratory methods for work with plant and soil nematodes. London, UKHMSO pp.  59-85.

  • HooperD.J. (1986c). Drawing and measuring nematodes. In: SoutheyJ.F. (Ed.). Laboratory methods for work with plant and soil nematodes. London, UKHMSO pp.  87-94.

  • LewisS.C.DyalL.A.HilburnC.F.WeitzS.LiauW.S.LaMunyonC.W.DenverD.R. (2009). Molecular evolution in Panagrolaimus nematodes: origins of parthenogenesis, hermaphroditism and the Antarctic species P. davidi. BMC Evolutionary Biology 915. DOI: 10.1186/1471-2148-9-15

  • McGillL.M.ShannonA.J.PisaniD.FélixM.A.RamløvH.DixI.WhartonD.A.BurnellA.M. (2015). Anhydrobiosis and freezing-tolerance: adaptations that facilitate the establishment of Panagrolaimus nematodes in polar habitats. PLoS ONE 10(3) e0116084. DOI: 10.1371/journal.pone.0116084

  • PiggottS.J.PerryR.N.WrightD.J. (2000). Hypo-osmotic regulation in entomopathogenic nematodes: Steinernema spp. and Heterorhabditis spp. Nematology 2561-566. DOI: 10.1163/156854100509349

  • PorazinskaD.L.WallD.H.VirginiaR.A. (2002). Invertebrates in ornithogenic soils on Ross Island, Antarctica. Polar Biology 25569-574. DOI: 10.1007/s00300-002-0386-7

  • RasbandW.S. (1997-2005). ImageJ. Available online at http://rsb.info.nih.gov/ij/.

  • RaymondM.R.WhartonD.A.MarshallC.J. (2013). Factors determining nematode distributions at Cape Hallett and Gondwana station, Antarctica. Antarctic Science 25347-357. DOI: 10.1017/S0954102012001162

  • RaymondM.R.WhartonD.A.MarshallC.J. (2014). Nematodes from the Victoria land coast, Antarctica and comparisons with cultured Panagrolaimus davidi. Antarctic Science 2615-22. DOI: 10.1017/S0954102013000230

  • SalehianS.BraeckmanB.P.BeladjalL.BertW.CleggJ.S.MertensJ. (2011). The importance of feeding status and desiccation rate in successful anhydrobiosis of Panagrolaimus detritophagus. Nematology 13185-191. DOI: 10.1163/138855410X512656

  • SmithT.WhartonD.A.MarshallC.J. (2008). Cold tolerance of an Antarctic nematode that survives intracellular freezing: comparisons with other nematode species. Journal of Comparative Physiology B–Biochemical Systemic and Environmental Physiology 17893-100. DOI: 10.1007/s00360-007-0202-3

  • StiernagleT. (1999). Maintenance of C. elegans. In: HopeI.A. (Ed.). C. elegans: a practical approach. Oxford, UKOxford University Press pp.  51-67.

  • TamuraK.StecherG.PetersonD.FilipskiA.KumarS. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 302725-2729. DOI: 10.1093/molbev/mst197

  • TimmR.W. (1971). Antarctic soil and freshwater nematodes from the McMurdo sound region. Proceedings of the Helminthological Society of Washington 3842-52.

  • WhartonD.A. (1998). Comparison of the biology and freezing tolerance of Panagrolaimus davidi, an Antarctic nematode, from field samples and cultures. Nematologica 44643-653. DOI: 10.1163/005725998X00050

  • WhartonD.A. (2003). The environmental physiology of Antarctic terrestrial nematodes: a review. Journal of Comparative Physiology B–Biochemical Systemic and Environmental Physiology 173621-628. DOI: 10.1007/s00360-003-0378-0

  • WhartonD.A. (2011). Cold tolerance. In: PerryR.N.WhartonD.A. (Eds). Molecular and physiological basis of nematode survival. Wallingford, UKCABI Publishing pp.  182-204.

  • WhartonD.A.BrownI.M. (1989). A survey of terrestrial nematodes from the McMurdo sound region, Antarctica. New Zealand Journal of Zoology 16467-470.

  • WhartonD.A.FernsD.J. (1995). Survival of intracellular freezing by the Antarctic nematode Panagrolaimus davidi. Journal of Experimental Biology 1981381-1387.

  • WhartonD.A.RaymondM.R. (2015). Cold tolerance of the Antarctic nematodes Plectus murrayi and Scottnema lindsayae. Journal of Comparative Physiology B–Biochemical Systemic and Environmental Physiology 185281-289. DOI: 10.1007/s00360-014-0884-2

  • YoderM.De LeyI.T.KingI.W.Mundo-OcampoM.MannJ.BlaxterM.PoirasL.De LeyP. (2006). DESS: a versatile solution for preserving morphology and extractable DNA of nematodes. Nematology 8367-376. DOI: 10.1163/156854106778493448

  • ZhangZ.SchwartzS.WagnerL.MillerW. (2000). A greedy algorithm for aligning DNA sequences. Journal of Computational Biology 7203-214. DOI: 10.1089/10665270050081478

Figures

  • View in gallery

    Map of Shackleton’s hut at Cape Royds showing where samples were collected for this study (map reproduced with permission of the Antarctic Heritage Trust). (Scale bar = 1 m.)

  • View in gallery

    Panagrolaimus sp. DAW1 (A, C) and female Royds 2 P. davidi (B, D), showing the dorsal metastomal tooth (A, B, arrows) and the tail (C, D). (Scale bars = 10 μm.)

Index Card

Content Metrics

Content Metrics

All Time Past Year Past 30 Days
Abstract Views 79 74 5
Full Text Views 175 175 1
PDF Downloads 7 7 0
EPUB Downloads 0 0 0