A new record of Xiphinema dentatum Sturhan, 1978 and description of X. paradentatum sp. n. (Nematoda: Dorylaimida) from Serbia

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A population of Xiphinema dentatum, found in a new locality in Serbia, is briefly described and illustrated. Xiphinema paradentatum sp. n. is described and illustrated. It is a parthenogenetic species characterised by its medium to moderately long body (3.71-5.02 mm), open C-shaped when heat-relaxed, lip region frontally almost flattened, laterally rounded and separated from the rest of the body by a weak depression; a moderately long odontostyle (147-166 μm), guide ring located at 137-153 μm from anterior end; two equally developed genital tracts having a pseudo-Z-organ in uterus in the form of several irregular angular sclerotised bodies – apophyses located in the vicinity of the pars dilatata uteri and devoid of spines; tail broadly and symmetrically rounded, and four juvenile developmental stages. Molecular characterisation using the ribosomal ITS and the D2-D3 expansion domains of the 28S rRNA gene was done and maximum likelihood analysis was used to reconstruct the phylogenetic relationships of X. dentatum and X. paradentatum sp. n. from Serbia with other longidorids.

A new record of Xiphinema dentatum Sturhan, 1978 and description of X. paradentatum sp. n. (Nematoda: Dorylaimida) from Serbia

in Nematology

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References

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Figures

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    Xiphinema dentatum. Entire body of female, male and four juvenile developmental stages.

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    Xiphinema dentatum, adults. A: Anterior region of female in lateral optical view; B, C: Female lip region; D: Amphid; E: Vulval region; F: Female anterior genital branch (p-z = pseudo-Z-organ); G1-G9: Pseudo-Z-organ in detail; H-K: Female tail region; L: Male posterior region. (Scale bars: A, L = 50 μm; B-E, G1-G9, H-K = 20 μm; F = 100 μm.)

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    Xiphinema dentatum. A1-A24: Pseudo-Z-organ in detail. (Scale bar = 20 μm.)

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    Xiphinema dentatum, juvenile stages. Anterior body region of J1 (A), J2 (B), J3 (C) and J4 (D); Tail regions of J1 (E, F), J2 (G-I), J3 (J, K) and J4 (L). (Scale bars: A-D = 50 μm; E-L = 20 μm.)

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    Scatter diagram separating juveniles (J1-J4) and females of Xiphinema dentatum from Deliblato Sand, Serbia.

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    Xiphinema paradentatum sp. n. View of entire body of female, male and four juvenile developmental stages.

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    Drawings of Xiphinema paradentatum sp. n. A, B: Entire body, female and male; C-F: Entire body, juveniles from J1 to J4, respectively; G: Lip region; H: Detail of basal pharyngeal bulb; I: Female anterior genital branch; J: Female tail region: K: Male tail region; L-O: Tail of juveniles from J1 to J4, respectively. (Scale bars: A-F = 200 μm; G, J-O = 20 μm; H, I = 50 μm.)

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    Xiphinema paradentatum sp. n., adults. A: Anterior region of female in lateral optical view; B, C: Female lip region; D: Amphid; E: Cylindrical basal bulb (cylindrus) of pharynx showing nuclei of dorsal and subventral glands (D = dorsal nucleus; glandularium = distance between dorsal nucleus and posterior margin of cylindrus – signified by two vertical black lines; AS1 = first anterior subventral nucleus; AS2 = second anterior subventral nucleus); F: Vulval region; G: Female posterior genital branch (p-z = pseudo-Z-organ); H1-H6: Pseudo-Z-organ in detail; I-L: Female tail region; M: Male posterior region. (Scale bars: A, E, F = 50 μm; B-D, H1-H5, I-L; G = 100 μm; M = 20 μm.)

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    Xiphinema paradentatum sp. n. A1-A12 and B1-B12: Pseudo-Z-organ in detail. (Scale bars = 20 μm.)

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    Xiphinema paradentatum sp. n., juvenile stages J1-J4. Anterior body region of J1 (A), J2 (B), J3 (C) and J4 (D); Tail region of J1 (E-H), J2 (I-L), J3 (M, N) and J4 (O, P). (Scale bars: A-D = 50 μm; E-P = 20 μm.)

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    Scatter diagram separating juveniles (J1-J4) and females of Xiphinema paradentatum sp. n. from Tara Mt., Hajdučka česma, Serbia.

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    Scatter diagram separating juveniles (J1-J4) and females of Xiphinema dentatum and X. paradentatum sp. n. from Serbia.

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    Ontogenesis of tail shape in two round-tailed species – Xiphinema dentatum and X. paradentatum sp. n. (J1, J2, J3, J4 = juvenile stages; ♀ = female). (Scale bars = 20 μm.)

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    Change of body diam. at anus, tail length and length of hyaline region of tail (J) during ontogenesis of tail shape in Xiphinema paradentatum sp. n. and X. dentatum. F = female; J1-J4 = juvenile stages. (Note: numbers represent mean values of used characters.)

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    Phylogenetic tree of the D2-D3 expansion domains of Xiphinema paradentatum sp. n. and its closest species. Sequences were analysed using the maximum likelihood method. Numbers at the nodes indicate the bootstrap values.

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    Phylogenetic tree of the ITS of Xiphinema paradentatum sp. n. and its closest species. Sequences were analysed using the maximum likelihood method. Numbers at the nodes indicate the bootstrap values.

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