Morphological and molecular characterisation of Longidorus juglans sp. nov. and a sister species L. fangi Xu & Cheng, 1991 (Nematoda: Longidoridae) from China

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Longidorus juglans sp. nov. is a new needle nematode detected from the rhizosphere of a walnut tree in Shanxi Province, P.R. China. It was distinguished by female body length of 3.9-5.2 mm; lip region 14-18 μm wide, rounded and continuous, amphidial fovea pocket-shaped without distinct basal lobes; moderately long odontostyle (125-140 μm); guide ring at mid-odontostyle and situated 69-78 μm from anterior end; short and blunt conoid tail (30-41 μm long, c = 118-147, c′ = 0.6-0.9), bearing 2-3 caudal pores on each side. The male of L. juglans sp. nov. is 5.1 mm long, with 13 ventro-median supplements, a short and blunt conoid tail bearing 3-4 caudal pores on each side, and spicules 73 μm long. Three developmental juvenile stages were recognised. This new species is very similar to another Chinese species L. fangi in general morphology, but can be distinguished by the number of juvenile stages and DNA sequences of SSU, ITS1, ITS2 and D2-D3 of LSU rDNA. Morphological and molecular characterisation was provided for L. fangi representing two new geographical records. The phylogenetic relationships among these species were analysed using data from SSU, D2-D3 of LSU, ITS1 and ITS2 rDNA, which revealed that L. juglans sp. nov. and L. fangi were clustered together with L. diadecturus, L. litchii and L. jonesi whose guide ring is located at mid-odontostyle level.

Morphological and molecular characterisation of Longidorus juglans sp. nov. and a sister species L. fangi Xu & Cheng, 1991 (Nematoda: Longidoridae) from China

in Nematology

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References

Archidona-YusteA.Navas-CortésJ.A.Cantalapiedra-NavarreteC.Palomares-RiusJ.E.CastilloP. (2016). Unravelling the biodiversity and molecular phylogeny of needle nematodes of the genus Longidorus (Nematoda: Longidoridae) in olive and a description of six new species. PloS ONE 11e0147689. DOI: 10.1371/journal.pone.0147689

BickfordD.LohmanD.J.SodhiN.S.NgP.K.MeierR.WinkerK.DasI. (2007). Cryptic species as a window on diversity and conservation. Trends in Ecology and Evolution 22148-155. DOI: 10.1016/j.tree.2006.11.004

ChenQ.HooperD.J.LoofP.A.A.XuJ. (1997). A revised polytomous key for the identification of the genus Longidorus Micoletzky, 1922 (Nematoda: Dorylaimoidea). Fundamental and Applied Nematology 2015-28.

ChiY.L.ZhangW.D.WuC.K.ShaoX.Y.LiaoL.ChenQ.W. (2011). [ Investigation of Longidoridae and Trichodoridae nematodes on landscape plants in Guangdong.] Plant Quarantine 2587-89.

DarribaD.TaboadaG.L.DoalloR.PosadaD. (2012). jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9772. DOI: 10.1038/nmeth.2109

DaviesK.A.Giblin-DavisR.M. (2004). The biology and association of Fergusobia (Nematoda) from the Melaleuca leucadendra-complex in eastern Australia. Invertebrate Systematics 18291-319. DOI: 10.1071/is02034

DecraemerW.CoomansA. (2007). Revision of some species of the genus Paralongidorus sensu Siddiqi et al. (1993), with a discussion on the relationships within the family Longidoridae (Nematoda: Dorylaimida). Nematology 9643-662. DOI: 10.1163/156854107782024776

De LucaF.ReyesA.GrunderJ.KunzP.AgostinelliA.De GiorgiC.LambertiF. (2004). Characterization and sequence variation in the rDNA region of six nematode species of the genus Longidorus (Nematoda). Journal of Nematology 36147-152.

De LucaF.LandaB.B.MifsudD.TroccoliA.VovlasN.CastilloP. (2009). Molecular characterisation of Longidorus kuiperi Brinkman, Loof & Barbez, 1987 (Nematoda: Longidoridae) from the Mediterranean Basin. Nematology 11155-160. DOI: 10.1163/156854108X398534

EdwardJ.C.MisraS.L.SinghG.R. (1964). Longidorus pisi n. sp. (Nematoda, Dorylaimoidea) associated with the rhizosphere of Pisum sativum, from Uttar Pradesh, India. Japanese Journal of Applied Entomology and Zoology 8310-312. DOI: 10.1303/jjaez.8.310

EsmaeiliM.HeydariR.Archidona-YusteA.CastilloP.Palomares-RiusJ.E. (2017). A new needle nematode, Longidorus persicus n. sp. (Nematoda: Longidoridae), from Kermanshah Province, western Iran. European Journal of Plant Pathology 14727-41. DOI: 10.1007/s10658-016-0976-9

EveleighE.S.AllenW.R. (1982). Description of Longidorus diadecturus n. sp. (Nematoda: Longidoridae), a vector of the peach rosette mosaic virus in peach orchards in southwestern Ontario, Canada. Canadian Journal of Zoology 60112-115. DOI: 10.1139/z82-014

GuindonS.GascuelO. (2003). A simple, fast and accurate method to estimate large phylogenies by maximum-likelihood. Systematic Biology 52696-704. DOI: 10.1080/10635150390235520

GuoK.ShiH.L.LiuK.ZhengJ.W. (2011). Past and present distribution and hosts of Longidorus (Nematoda: Dorylaimida) in mainland China. Zootaxa 308627-38.

Gutiérrez-GutiérrezC.Palomares-RiusJ.E.Cantalapiedra-NavarreteC.LandaB.B.EsmenjaudD.CastilloP. (2010). Molecular analysis and comparative morphology to resolve a complex of cryptic Xiphinema species. Zoologica Scripta 39483-498. DOI: 10.1111/j.1463-6409.2010.00437.x

Gutiérrez-GutiérrezC.Cantalapiedra-NavarreteC.DecraemerW.VovlasN.PriorT.Palomares-RiusJ.E.CastilloP. (2012). Phylogeny, diversity, and species delimitation in some species of the Xiphinema americanum-group complex (Nematoda: Longidoridae), as inferred from nuclear and mitochondrial DNA sequences and morphology. European Journal of Plant Pathology 134561-597. DOI: 10.1007/s10658-012-0039-9

Gutiérrez-GutiérrezC.Cantalapiedra-NavarreteC.Montes-BorregoM.Palomares-RiusJ.E.CastilloP. (2013). Molecular phylogeny of the nematode genus Longidorus (Nematoda: Longidoridae) with description of three new species. Zoological Journal of the Linnean Society 167473-500. DOI: 10.1111/zoj.12019

HeynsJ. (1966). Further studies on south African Longidoridae (Nematoda). South African Journal of Agricultural Science 9927-943.

HeynsJ.SwartA. (1998). Longidorus jagerae sp. n., another longidorid species with globular inclusions in the prerectum (Nematoda: Longidoridae). Koedoe 4131-36. DOI: 10.4102/koedoe.v41i1.243

HeynsJ.CoomansA.HutsebautM.SwartA. (1987). Longidorus fursti n. sp. from South Africa with a discussion of its relationships (Nematoda: Longidoridae). Fundamental and Applied Nematology 10381-385.

HirataK. (1995). Descriptions of two new species of Longidorus (Dorylaimida: Longidoridae) from Nagano, Japan. Japanese Journal of Nematology 2533-43. DOI: 10.3725/jjn1993.25.1_33

HirataK. (2002). Description of a new species of Longidorus (Dorylaimida: Longidoridae) from Ishigaki Is., Japan. Japanese Journal of Nematology 321-6. DOI: 10.3725/jjn1993.32.1_1

HooperD.J. (1986). Handling, fixing, staining and mounting nematodes. In: SoutheyJ.F. (Ed.). Laboratory methods for work with plant and soil nematodes. Reference Book 402 HMSO. London, UKMAFF pp.  59-80.

KhanE. (1986). One new genus and four new species in the super family Longidoroidea (Nematoda). Indian Journal of Nematology 16185-193.

KozlowskaJ.SeinhorstJ.W. (1979). Longidorus elongatus and closely related species in the Netherlands and lower Saxony (Germany), with the description of two new species, L. cylindricaudatus and L. intermedius (Nematoda: Dorylaimida). Nematologica 2542-53. DOI: 10.1163/187529279X00361

LargetB.SimonD.L. (1999). Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution 16750-759. DOI: 10.1093/oxfordjournals.molbev.a026160

LucM.CoomansA. (1988). Xiphinema smoliki n. sp. and Longidorus pawneensis n. sp. (Nemata: Longidoridae) from Colorado, USA. Revue de Nématologie 11137-142.

MernyG. (1966). Nématodes d’Afrique tropicale: un nouveau Paratylenchus (Criconematidae), deux nouveaux Longidorus et observations sur Longidorus laevicapitatus Williams, 1959 (Dorylaimidae). Nematologica 12385-395. DOI: 10.1163/187529266X00860

NunnG.B. (1992). Nematode molecular evolution. Ph.D. Thesis. University of Nottingham Nottingham UK.

Palomares-RiusJ.E.Cantalapiedra-NavarreteC.CastilloP. (2014). Cryptic species in plant-parasitic nematodes. Nematology 161105-1118. DOI: 10.1163/15685411-00002831

PenevaV.K.LazarovaS.S.De LucaF.BrownD.J.F. (2013). Description of Longidorus cholevae sp. n. (Nematoda, Dorylaimida) from a riparian habitat in the Rila Mountains, Bulgaria. Zookeys 151-26. DOI: 10.3897/zookeys.330.5750

RobbinsR.T.BrownD.J.F. (1991). Comments on the taxonomy, occurrence and distribution of Longidoridae (Nematoda) in North America. Nematologica 37395-419. DOI: 10.1163/187529291X00402

RobbinsR.T.YeW.PedramM. (2009). Longidorus ferrisi n. sp. from California citrus. Journal of Nematology 41104-110.

RonquistF.HuelsenbeckJ.P. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 191572-1574. DOI: 10.1093/bioinformatics/btg180

Roshan-BakhshA.PourjamE.PedramM. (2016). Description of Longidorus perangustus sp. n. (Dorylaimida: Longidoridae), an amphimictic species from Iran. European Journal of Plant Pathology 144581-594. DOI: 10.1007/s10658-015-0796-3

SeinhorstJ.W. (1959). A rapid method for the transfer of nematodes from fixative to anhydrous glycerine. Nematologica 467-69. DOI: 10.1163/187529259X00381

SiddiqiM.R. (1962). Studies on the genus Longidorus Micoletzky, 1922 (Nematoda: Dorylaimoidea), with descriptions of three new species. Proceedings of the Helminthological Society of Washington 29177-188.

SzalanskiA. (2004). Phylogenetic relationships of Longidoridae species (Nematoda: Dorylaimida) from North America inferred from 18S rDNA sequence data. Helminthologia 41209-215.

ThompsonJ.D.GibsonT.J.PlewniakF.JeanmouginF.HigginsD.G. (1997). The clustal x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 254876-4882. DOI: 10.1093/nar/25.24.4876

TrisciuzziN.Archidona-YusteA.TroccoliA.FanelliE.De LucaF.VovlasN.CastilloP. (2015). Description of a new needle nematode, Longidorus asiaticus n. sp. (Nematoda: Longidoridae), from the rhizosphere of crape myrtle (Lagerstroemia indica) bonsai trees imported into Italy from China. European Journal of Plant Pathology 143567-580. DOI: 10.1007/s10658-015-0710-z

VrainT.C.WakarchukD.A.LevesqueA.HamiltonI. (1992). Intraspecific DNA restriction fragment length polymorphism in the Xiphinema americanum group. Fundamental and Applied Nematology 15563-573.

WangS.ChiuW.F.YuC.C.LiC.M.RobbinsR.T. (1996). The occurrence and geographical distribution of longidorid and trichodorid nematodes associated with vineyards and orchards in China. Russian Journal of Nematology 4145-154.

WhiteheadA.G.HemmingJ.R. (1965). A comparison of some quantitative methods for extracting small vermiform nematodes from soil. Annals of Applied Biology 5525-38. DOI: 10.1111/j.1744-7348.1965.tb07864.x

WilliamsB.D.SchrankB.HuynhC.ShownkeenR.WaterstonR.H. (1992). A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites. Genetics 131609-624.

XuJ.HooperD.J. (1990). Observations on some species of Longidorus (Nematoda: Longidoridae) from Jiangsu Province, China, with a description of Longidorus jiangsuensis n. sp. Revue de Nématologie 13323-330.

XuJ.H.ChengH.R. (1991). [ A new species of Longidorus (Nematoda: Longidoridae).] Journal of Nanjing Agricultural University 1438-42.

XuJ.H.ChengH. (1992). Longidorus litchii n. sp. and L. henanus n. sp. (Nemata: Longidoridae) from China. Fundamental and Applied Nematology 15517-523.

YagitaH. (1975). The life history and biology of the needle nematode, Longidorus martini Merny. I. Morphological measurements and developmental stages. Japanese Journal of Nematology 510-15.

YeW.SzalanskiA.L.RobbinsR.T. (2004). Phylogenetic relationships and genetic variation in Longidorus and Xiphinema species (Nematoda: Longidoridae) using ITS1 sequences of nuclear ribosomal DNA. Journal of Nematology 3614-19.

YeatesG.W.Van EttegerM.HooperD.J. (1992). Longidorus orongorongensis n. sp. (Nematoda: Dorylaimida) from subsoil of conifer/broadleaved forest. New Zealand Journal of Zoology 1925-31. DOI: 10.1080/03014223.1992.10423248

YeatesG.W.BoagB.BrownD.J.F. (1997). Two new species of Longidoridae (Nematoda) from New Zealand forests. Systematic Parasitology 3833-43. DOI: 10.1023/A:1005859707719

ZhaiL.X.LiX.D.XieH. (1999). [ Identification of two species of Longidorus and Xiphidorus (Nematoda: Dorylaimida) from rhizosphere of banana in Yunnan.] Journal of Huazhong Agricultural University 1833-36.

ZhangS.S.ChenY.B. (1994). [ Studies on the nematodes of bayberry (Myrica rubra).] Journal of Fujian Agricultural University (Natural Science Edition) 23172-177.

ZhengJ.PenevaV.BrownD.J.F. (2000). Longidorus camelliae n. sp. (Nematoda: Longidoridae) associated with ornamental cultivars of Camellia japonica L. growing in a nursery at Fuyang, Zhejiang Province, eastern China. Systematic Parasitology 47119-125. DOI: 10.1023/A:1006482322716

ZhengJ.PengD.RobbinsR.T.BrownD.J.F. (2001). Description of Longidorus hangzhouensis sp. n. (Nemata: Longidoridae) from Zhejiang province, new geographical records of L. henanus Xu & Cheng, 1992, and an identification key for Longidorus species occurring in China. Nematology 3219-227. DOI: 10.1163/156854101750413298

ZhengJ.W.PanC.S.FurlanettoC.NeilsonR.BrownD.J.F. (2002a). The morphology of the odontophore of Longidorus litchii Xu & Cheng, 1992 (Nematoda: Longidoridae). Russian Journal of Nematology 10123-126.

ZhengJ.W.SubbotinS.A.HeS.S.GuJ.F.MoensM. (2002b). Molecular characterisation of some Asian isolates of Bursaphelenchus xylophilus and B. mucronatus using PCR-RFLPs and sequences of ribosomal DNA. Russian Journal of Nematology 1117-22.

Figures

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    Light micrographs of Longidorus juglans sp. nov. A: Female anterior region; B: Amphidial fovea; C: Nerve ring (arrowed); D: Base of pharynx (arrowed); E: Pharyngeal bulb; F: Ovary; G: Vulval region, subventral view; H: Female tail; I: Part of male genital system; J: Male tail showing supplements and spicules. (Scale bars: A, C, G, H, I = 20 μm; F, J = 50 μm; B, D, E = 10 μm.)

  • View in gallery

    Line drawing of Longidorus juglans sp. nov. A, B: Male anterior region; C: Male pharyngeal bulb; D: Posterior genital branch; E: Female tail; F: Male tail; G: Male habitus; H: Female habitus. (Scale bars: A, B, D-F = 50 μm; C = 20 μm; G, H = 1 mm.)

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    Light micrographs of Longidorus juglans sp. nov. juveniles. A-C: J1-J3 anterior region, respectively; D-F: J1-J3 tail region, respectively.

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    Scatter plot of the functional and replacement odontostyle in relation to body length of Longidorus juglans sp. nov. juvenile developmental stages and females.

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    Light micrographs of Longidorus fangi female. A: Anterior region; B: Odontophore base; C: Vulval region; D-G: Tail shape. (Scale bars = 25 μm.)

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    Bayesian consensus tree inferred from SSU under GTR + I + G model. Posterior probability values exceeding 50% are given on appropriate clades. Newly obtained sequences in this study are in bold.

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    Bayesian consensus tree inferred from D2-D3 under GTR + I + G model. Posterior probability values exceeding 50% are given on appropriate clades. Newly obtained sequences in this study are in bold.

  • View in gallery

    Bayesian consensus tree inferred from ITS1 under GTR + I + G model. Posterior probability values exceeding 50% are given on appropriate clades. Newly obtained sequences in this study are in bold.

  • View in gallery

    Bayesian consensus tree inferred from ITS2 under GTR + I + G model. Posterior probability values exceeding 50% are given on appropriate clades. Newly obtained sequences in this study are in bold.

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