Morphological and molecular characterisation of Longidorus juglans sp. nov. and a sister species L. fangi Xu & Cheng, 1991 (Nematoda: Longidoridae) from China

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Longidorus juglans sp. nov. is a new needle nematode detected from the rhizosphere of a walnut tree in Shanxi Province, P.R. China. It was distinguished by female body length of 3.9-5.2 mm; lip region 14-18 μm wide, rounded and continuous, amphidial fovea pocket-shaped without distinct basal lobes; moderately long odontostyle (125-140 μm); guide ring at mid-odontostyle and situated 69-78 μm from anterior end; short and blunt conoid tail (30-41 μm long, c = 118-147, c′ = 0.6-0.9), bearing 2-3 caudal pores on each side. The male of L. juglans sp. nov. is 5.1 mm long, with 13 ventro-median supplements, a short and blunt conoid tail bearing 3-4 caudal pores on each side, and spicules 73 μm long. Three developmental juvenile stages were recognised. This new species is very similar to another Chinese species L. fangi in general morphology, but can be distinguished by the number of juvenile stages and DNA sequences of SSU, ITS1, ITS2 and D2-D3 of LSU rDNA. Morphological and molecular characterisation was provided for L. fangi representing two new geographical records. The phylogenetic relationships among these species were analysed using data from SSU, D2-D3 of LSU, ITS1 and ITS2 rDNA, which revealed that L. juglans sp. nov. and L. fangi were clustered together with L. diadecturus, L. litchii and L. jonesi whose guide ring is located at mid-odontostyle level.

Morphological and molecular characterisation of Longidorus juglans sp. nov. and a sister species L. fangi Xu & Cheng, 1991 (Nematoda: Longidoridae) from China

in Nematology



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    Light micrographs of Longidorus juglans sp. nov. A: Female anterior region; B: Amphidial fovea; C: Nerve ring (arrowed); D: Base of pharynx (arrowed); E: Pharyngeal bulb; F: Ovary; G: Vulval region, subventral view; H: Female tail; I: Part of male genital system; J: Male tail showing supplements and spicules. (Scale bars: A, C, G, H, I = 20 μm; F, J = 50 μm; B, D, E = 10 μm.)

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    Line drawing of Longidorus juglans sp. nov. A, B: Male anterior region; C: Male pharyngeal bulb; D: Posterior genital branch; E: Female tail; F: Male tail; G: Male habitus; H: Female habitus. (Scale bars: A, B, D-F = 50 μm; C = 20 μm; G, H = 1 mm.)

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    Light micrographs of Longidorus juglans sp. nov. juveniles. A-C: J1-J3 anterior region, respectively; D-F: J1-J3 tail region, respectively.

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    Scatter plot of the functional and replacement odontostyle in relation to body length of Longidorus juglans sp. nov. juvenile developmental stages and females.

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    Light micrographs of Longidorus fangi female. A: Anterior region; B: Odontophore base; C: Vulval region; D-G: Tail shape. (Scale bars = 25 μm.)

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    Bayesian consensus tree inferred from SSU under GTR + I + G model. Posterior probability values exceeding 50% are given on appropriate clades. Newly obtained sequences in this study are in bold.

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    Bayesian consensus tree inferred from D2-D3 under GTR + I + G model. Posterior probability values exceeding 50% are given on appropriate clades. Newly obtained sequences in this study are in bold.

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    Bayesian consensus tree inferred from ITS1 under GTR + I + G model. Posterior probability values exceeding 50% are given on appropriate clades. Newly obtained sequences in this study are in bold.

  • View in gallery

    Bayesian consensus tree inferred from ITS2 under GTR + I + G model. Posterior probability values exceeding 50% are given on appropriate clades. Newly obtained sequences in this study are in bold.


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