The commercial use of the entomopathogenic nematode Heterorhabditis bacteriophora as a biocontrol agent against noxious insects is limited due to its relatively short shelf-life. Longevity of dauer juveniles (DJ) during storage and in transit to end users is considerably restricted by environmental stresses. As a derivative stress triggered by environmental factors, oxidative stress causes a strong internal metabolic imbalance leading to lifespan reduction. In this study, the relation between DJ oxidative stress tolerance and longevity in H. bacteriophora was investigated at 25 and 7°C. A strong and significant correlation between DJ oxidative stress tolerance and longevity during storage in Ringer’s solution ( at 7°C; at 25°C) was recorded. Phenotyping of these traits was performed for 40 H. bacteriophora wild type strain and inbred line collections. At 25°C, the mean time survived in Ringer’s by 50% of the DJ (MTS50) ranged from 21 to 57 days, whereas under oxidative stress, survival was from 3 to 22 days. At 7°C, a maximum MTS50 of 94 days was assessed when DJ were stored in Ringer’s, while the maximum MTS50 was only 25 days with oxidative stress induction. The heritability of DJ tolerance to oxidative stress, determined by using homozygous inbred lines, is high (), an indication of a high probability for successful selective breeding. In a subset of preselected H. bacteriophora inbred lines, DJ oxidative stress tolerance correlated with the DJ survival (persistence) after application to sand (). The study provides fundamental data required for a genetic breeding programme to produce hybrids with improved stress tolerance and prolonged shelf-life and soil persistence.
Effect of the tocotrienol-rich fraction on the lifespan and oxidative biomarkers in Caenorhabditis elegans under oxidative stress.
Clinics68599-604. DOI: 10.6061/clinics/2013(05)04
Stabilisation of heat tolerance traits in Heterorhabditis bacteriophora through selective breeding and creation of inbred lines in liquid culture.
BioControl5885-93. DOI: 10.1007/s10526-012-9467-x
Influence of temperature and duration of storage on the lipid reserves of entomopathogenic nematodes.
Revista Colombiana de Entomología37203-209.
BernerM.EhlersR.-U.SchnetterW. (2001). Genetic variability and discrimination of isolates inbred lines and hybrids of Heterorhabditis bacteriophoravia RAPD-PCR. In: 34th Annual Meeting of the Society for Invertebrate Pathology. Noordwijkerhout The Netherlands August 25-30 p. 7.
Genetic specification of life span and self-fertility in recombinant-inbred strains of Caenorhabditis elegans.
Heredity6719-28. DOI: 10.1038/hdy.1991.60
Estimating the nematode population of soil with special reference to the sugar-beet and root-gall nematodes Heterodera schachtii Schmidt and Heterodera radicicola (Greef) Müller and with a description of Tylencholaimus aequalis n. sp. Washington DC USAGovernment Printing Office.
Molecular and cellular mechanisms of iron homeostasis and toxicity in mammalian cells.
Journal of Inorganic Biochemistry919-18. DOI: 10.1016/S0162-0134(02)00461-0
Multiple stressors in Caenorhabditis elegans induce stress hormesis and extended longevity.
The Journals of Gerontology Series A: Biological Sciences and Medical Sciences57B109-B114. DOI: 10.1093/gerona/57.3.B109
Genetic selection for heat tolerance and low temperature activity of the entomopathogenic nematode-bacterium-complex Heterorhabditis bacteriophora-Photorhabdus luminescens.
Biocontrol50699-716. DOI: 10.1007/s10526-005-5079-z
Formulation and quality control of entomopathogenic nematodes. In:
Nematodes as biocontrol agents. Wallingford, UKCAB International pp.
79-90. DOI: 10.1079/9780851990170.0079
Dauer juvenile longevity and stress tolerance in natural populations of entomopathogenic nematodes: is there a relationship?International Journal for Parasitology32717-725. DOI: 10.1016/S0020-7519(02)00029-2
Longevity and stress tolerance. In:
Molecular and physiological basis of nematode survival. Wallingford, UKCAB International pp.
157-181. DOI: 10.1079/9781845936877.0157
Heritability of the liquid culture mass production potential of the entomopathogenic nematode Heterorhabditis bacteriophora.
Biocontrol Science and Technology12267-276. DOI: 10.1080/09583150120124504
JohnsonT.E.de CastroE.de CastroS.H.CypserJ.R.HendersonS.TedescoP.M. (2001).
Relationship between increased longevity and stress resistance as assessed through gerontogene mutations in Caenorhabditis elegans.
JohnsonT.E.HendersonS.MurakamiS.de CastroE.de CastroS.H.CypserJ.RikkeB.TedescoP.LinkC. (2002).
Longevity genes in the nematode Caenorhabditis elegans also mediate increased resistance to stress and prevent disease.
Journal of Inherited Metabolic Disease25197-206. DOI: 10.1023/A:1015677828407
Quantitative in vivo redox sensors uncover oxidative stress as an early event in life.
Molecular Cell47767-776. DOI: 10.1016/j.molcel.2012.06.016
Effects of oxidative stress on behavior, physiology, and the redox thiol proteome of Caenorhabditis elegans.
Antioxidants & Redox Signaling141023-1037. DOI: 10.1089/ars.2010.3203
Assessment of establishment and persistence of entomopathogenic nematodes for biological control of western corn rootworm.
Journal of Applied Entomology131420-425. DOI: 10.1111/j.1439-0418.2007.01202.x
Lipid utilisation during storage of the entomopathogenic nematodes Steinernema feltiae and Heterorhabditis megidis from Greece and the UK.
Nematology531-37. DOI: 10.1163/156854102765216669
Heat tolerance among different strains of the entomopathogenic nematode Heterorhabditis bacteriophora.
BioControl55423-434. DOI: 10.1007/s10526-009-9255-4
Variability in desiccation tolerance among different strains of the entomopathogenic nematode Heterorhabditis bacteriophora.
Nematology12711-720. DOI: 10.1163/138855409X12607871174454
Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans.
Nature424277-283. DOI: 10.1038/nature01789
Life cycle and calculation of the intrinsic rate of natural increase of two bacterivorous nematodes, Panagrolaimus sp. and Poikilolaimus sp. from chemoautotrophic Movile Cave, Romania.
Nematology9271-284. DOI: 10.1163/156854107780739117
Hybridisation and selective breeding for improvement of low temperature activity of the entomopathogenic nematode Steinernema feltiae.
BioControl58417-426. DOI: 10.1080/09583157.2012.758238
A redox-sensitive peroxiredoxin that is important for longevity has tissue- and stress-specific roles in stress resistance.
Proceedings of the National Academy of Sciences of the United States of America10519839-19844. DOI: 10.1073/pnas.0805507105
Persistence of the entomoparasitic nematode Heterorhabditis bacteriophora in maize fields.
Journal of Applied Entomology138202-212. DOI: 10.1111/j.1439-0418.2012.01743.x
The role of hydrogen peroxide in regulation of plant metabolism and cellular signalling in response to environmental stresses.
Acta Biochimica Polonica – English Edition5439-50.
Storage and formulation of the entomopathogenic nematodes Heterorhabditis indica and H. bacteriophora.
BioControl45483-500. DOI: 10.1023/A:1026528727365
Genetic improvement of the desiccation tolerance of the entomopathogenic nematode Heterorhabditis bacteriophora through selective breeding.
BioControl31218-226. DOI: 10.1016/j.biocontrol.2004.03.009
ThamsenM. (2011). The role of oxidative stress in C. elegans aging. Ph.D. Thesis Johannes Gutenberg-University Mainz Germany.
Controlling Western Corn Rootworm larvae with entomopathogenic nematodes: effect of application techniques on plant-scale efficacy.
Journal of Applied Entomology134467-480. DOI: 10.1111/j.1439-0418.2009.01469.x
Adaptive responses to oxidative damage in three mutants of Caenorhabditis elegans (age-1, mev-1 and daf-16) that affect life span.
Mechanisms of Ageing and Development1231579-1587. DOI: 10.1016/S0047-6374(02)00093-3