Two new species of Heth Cobb, 1898 (Nematoda: Ransomnematoidea) from Northern Mindanao, the Philippines

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Heth konoplevi n. sp. and Heth initiensis n. sp. are described from an unidentified spirobolid (Rhinocricidae) millipede collected at Mindanao Island, the Philippines. Females of H. konoplevi n. sp. are characterised by the absence of lateral cuticular lappets and the arrangement of lateral spines with an unpaired one in mid-lateral position that brings it closer to H. zeuglocantha from Papua New Guinea. The presence of a cervical collar, lateral cuticular lappets, two pairs of lateral cuticular spines in females and a bursa-like extension around the cloacal region in males of H. initiensis n. sp. makes it closer to some representatives of the genus from the Asia-Pacific and Australasian regions. The description of H. initiensis n. sp. is supplemented by details of the fourth-stage juvenile morphology. Molecular phylogenetic analysis of SSU and LSU rDNA is provided.

Two new species of Heth Cobb, 1898 (Nematoda: Ransomnematoidea) from Northern Mindanao, the Philippines

in Nematology

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References

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Figures

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    Heth konoplevi n. sp. A: Female, entire view; B: Female anterior end, lateral view; C, D: Optical section through female buccal cavity in lateral and dorso-ventral view, respectively; E: Superficial structures of vulval region, lateral view; F: Female excretory duct, anterior end; G: Male, entire view; H: Male anterior end, lateral view; I, J: Optical section through male buccal cavity; K: Spicule and gubernaculum; L: Cloacal region, lateral view; M: Male tail end. (Scale bars: A, G = 325 μm; B, I, J, L, M = 40 μm; C-E = 60 μm; F = 20 μm; H = 80 μm; K = 25 μm.)

  • View in gallery

    SEM micrographs of Heth konoplevi n. sp. A: Female anterior end, ventro-lateral view; B: Female anterior end, lateral view; C: Female oral opening, apical view; D: Female vulval region, ventral view; E: Vulva, anterior lip; F: Male anterior end, apical view; G, H: Male posterior end, ventral view. (Scale bars: A, H = 25 μm; B = 15 μm; C, F = 5 μm; D, G = 100 μm; E = 10 μm.)

  • View in gallery

    Heth initiensis n. sp. A: Female, entire view; B: Female anterior end, lateral view; C, D: Optical section through female buccal cavity in lateral and dorso-ventral view, respectively; E: Vulval region, lateral view; F: Female excretory duct, anterior end; G: Male, entire view; H: Male anterior end, lateral view; I, J: Optical section through male buccal cavity; K: Spicule and gubernaculum; L: Optical section through male posterior region; M: Cloacal region, lateral view; N: Fourth-stage (J4) female juvenile, entire view; O: J4 female gonad distal tip; P: J4 female vulval primordium, lateral view. (Scale bars: A, N = 650 μm; B, E, L, O, P = 150 μm; C, D = 90 μm; F = 100 μm; G = 750 μm; H = 170 μm; I-K = 80 μm; M = 240 μm.)

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    SEM micrographs of Heth initiensis n. sp. A: Female anterior end, apical view; B: Female anterior end, lateral view; C: Male head, lateral view; D: Male posterior end, lateral view; E: Cuticularised median duct on anterior cloacal lip; F, G: Male posterior end, ventral view. (Scale bars: A = 25 μm; B = 50 μm; C = 10 μm; D, G = 100 μm; E = 5 μm; F = 50 μm.)

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    Phylogenetic relationships among studied species of superfamily Ransomnematoidea based on partial SSU and LSU rDNA sequences. Values of bootstrap support are given in the form ML/NJ/MP. A: Analysis inferred from partial sequence of SSU rDNA. All analyses are based on a dataset of 1630 total characters. B: Analysis inferred from partial sequence of D2-D3 expansion segment of LSU rDNA. All analyses are based on a dataset of 1013 total characters. Newly obtained sequences are indicated in bold.

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