Description of Ruehmaphelenchus quercophilus n. sp. (Nematoda: Aphelenchoididae) from dying oak, Quercus robur, in Poland

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Ruehmaphelenchus quercophilus n. sp., isolated from a dying oak, Quercus robur, in the State Forests, Łopuchówko District, Poland, is described and figured. It is characterised by three lateral lines, relatively stout body, excretory pore posterior to nerve ring, five caudal papillae, spicules relatively small (12-14 μm) with weakly developed condylus and rostrum, bursa absent, vulva positioned at ca 78% of body length, vulval flap absent, post-uterine branch extending for slightly over half of vulva to anus distance, and conoid tail with a bend at the distal end forming a terminal projection 10-14 μm long. The new species is very similar to four other described species, namely R. formosanus, R. juliae, R. thailandae, and R. sirisus. Detailed phylogenetic analysis based on 18S, 28S D2-D3 and ITS sequences has confirmed the status of this nematode as a new species.

Description of Ruehmaphelenchus quercophilus n. sp. (Nematoda: Aphelenchoididae) from dying oak, Quercus robur, in Poland

in Nematology



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    Ruehmaphelenchus quercophilus n. sp. A: Entire female; B: Entire male; C: Anterior body; D: Lateral lines; E: Gonad; F: Male tail in ventral view showing caudal papillae (P1-P3, arrows); G: Male tail in lateral view; H: Spicules; I: Female tail; J, K: Female tail terminus. (Scale bars = 10 μm.)

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    Light photomicrographs of Ruehmaphelenchus quercophilus n. sp. male. A: Entire body; B: Anterior region; C: Pharyngeal region; D-G: Tail in lateral view showing spicules; H: Tail in ventral view showing position of papillae. (Scale bars = 10 μm.)

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    Light photomicrographs of Ruehmaphelenchus quercophilus n. sp. female. A: Entire body; B: Posterior region showing reproductive region; C: Head region; D: Lateral lines; E: Vulval region; F-H: Female tail. (Scale bars = 10 μm.)

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    Scanning electron microscopy of Ruehmaphelenchus quercophilus n. sp. Aa, Ab: Head region in different views; B: Lateral lines; C: Vulval region; D: Female tail; E: Male tail (ventral, showing papillal arrangement).

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    Phylogenetic relationships of Ruehmaphelenchus quercophilus n. sp. and aphelenchid nematodes based on concatenated dataset (partial 18S rDNA + full length of ITS rDNA + partial 28S D2-D3 rDNA). The 10 001st Bayesian tree was inferred under GTR + I + G model, Aphelenchoides besseyi served as the outgroup species. Posterior probability values exceeding 50% are given on appropriate clades.

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