On the identity of Chiloplacus subtenuis Rashid & Heyns, 1990 and C. tenuis Rashid & Heyns, 1990 (Rhabditida: Cephalobidae), with description of C. mysteriosus sp. n.

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Type material of Chiloplacus subtenuis and C. tenuis was studied and Iberian material originally described as C. tenuis re-examined. The results provided relevant new information to elucidate the identity of these species with more accuracy. On studying their type material, new features and details of C. subtenuis and C. tenuis were observed concerning the nature of the lip margin, morphology of the labial probolae, the lateral field at vulval level, and the orientation of the vulva (ventral or subventral). Re-examination of Iberian material of C. tenuis revealed that it consists of specimens belonging to three taxa: C. tenuis, C. membranifer, and an unknown species described and named herein as C. mysteriosus sp. n. The morphology of lip region in representatives of Chiloplacus is analysed and discussed. Zeldia spannata is transferred to Chiloplacus as C. spannatus comb. n. and Chiloplacus juniperi is regarded as a junior synonym of Nothacrobeles nanocorpus.

Nematology

International Journal of Fundamental and Applied Nematological Research

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References

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Figures

  • Chiloplacus subtenuis Rashid & Heyns, 1990 (paratypes, light microscope). A: Neck; B-E: Anterior end (arrows indicating acute tip of primary axils, I = primary axil, II = secondary axil); F: Female reproductive system; G, H: Vagina (arrow pointing at vulva); I, J: Female posterior end (arrow pointing at phasmid).

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  • Chiloplacus tenuis Rashid & Heyns, 1990 (paratypes, light microscope). A: Neck; B, C: Anterior end (I = primary axil, II = secondary axil); D: Lateral field; E: Female reproductive system; F-H: Mid-body at right lateral field level, vulval level, left lateral field level (dark arrow pointing to vulva, r.s. = right side, l.s. = left side); I: Vulva and left lateral field; J, K: Female posterior end (arrow pointing to phasmid); L: Male posterior end (white arrows pointing to post-cloacal genital papillae, dark arrow pointing to phasmid).

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  • Chiloplacus tenuis Rashid & Heyns, 1990 from Spain (light microscope). A, B: Anterior end at lips and stoma levels, respectively; C: Neck with detail of deirid; D: Female reproductive system at post-vulval sac level; E, F: Left and right lateral fields at vulva level, respectively; G: Female posterior end (arrow pointing at phasmid); H: Male posterior end. Chiloplacus membranifer Holovachov, Boström, Mundo-Ocampo & Villenave, 2008 from Spain (light microscope). I: Neck with detail of deirid (inset); J, K: Anterior end at lips and stoma levels, respectively; L: Lateral field; M, N: Left and right lateral fields at vulval level, respectively (arrow pointing at lateral field expansion); O: Female posterior end (arrow pointing at phasmid); P: Male posterior end. Chiloplacus mysteriosus sp. n. (light microscope). Q: Neck with detail of deirid (inset); R, S: Anterior end at lips and stoma levels, respectively; T: Lateral field; U, V: Left and right lateral fields at vulva level, respectively; W: Female posterior end (arrow pointing at phasmid); X: Female reproductive system at post-vulval sac level.

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  • Chiloplacus mysteriosus sp. n. (female). A: Neck; B: Anterior end; C: Lip region; D: Reproductive system; E: Lateral field; F: Entire female; G: Posterior end.

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  • Chiloplacus mysteriosus sp. n. (scanning electron microscope). A, B, D, E: Lip region in left lateral, subfrontal, left subventral and dorsal views, respectively; C: Anterior end; F: Deirid; G: Lateral field at mid-body.

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  • Variation observed in Chiloplacus. Lip region of the symmetricus-group (A), minimus-group (B), tauricus-group (C), Stegelletina (D) and Zeldia (E). Lip region of C. subtenuis (F), C. mysteriosus sp. n. (G), C. tauricus (H) and C. spannatus (I). Vulva of C. mysteriosus sp. n. (J), C. tauricus (K) and C. spannatus (L). Posterior end of female of C. subtenuis (M), C. mysteriosus sp. n. (N), C. tauricus (O) and C. spannatus (P).

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