Description of Xiphinema hangzhouense sp. n. (Nematoda: Longidoridae) from the rhizosphere of Magnolia grandiflora in Hangzhou, Zhejiang Province, China

in Nematology
Restricted Access
Get Access to Full Text
Rent on DeepDyve

Have an Access Token?



Enter your access token to activate and access content online.

Please login and go to your personal user account to enter your access token.



Help

Have Institutional Access?



Access content through your institution. Any other coaching guidance?



Connect

Xiphinema hangzhouense sp. n. is described from the rhizosphere of Magnolia grandiflora in Hangzhou, China. The new species is characterised by having a monodelphic reproductive system, slim body, total stylet = 186-192 μm long and vulva located anteriorly at 27.6-31.2% of total body length. The new species has a gonad 400-450 μm long with a simple uterus and no Z-organ. The female tail is elongated conoid with a rounded terminus. The species has four juvenile stages and no male was found. The polytomous identification codes of X. hangzhouense sp. n. are A1, B4, C2, D23, E12, F2, G2, H2, I23, J2, K2, L1. The new species is morphologically similar to X. winotoi. Phylogenetic analyses of the 28S rRNA, ITS rRNA, 18S rRNA and partial COI gene sequences of X. hangzhouense sp. n. and some closely related Xiphinema species revealed that the new taxon clustered with high statistical support with X. radicicola and X. setariae.

Nematology

International Journal of Fundamental and Applied Nematological Research

Sections

References

BrownD.J.F.TophamP.B. (1985). Morphometric variability between populations of Xiphinema diversicaudatum (Nematoda: Dorylaimoidea). Revue de Nématologie 8, 15-26.

BrownR.H. (1968). Xiphinema monohysterum n. sp. (Nematoda: Dorylaimoidea) from southern New South Wales. Nematologica 13, 633-637. DOI: 10.1163/187529267X00454

CobbN.A. (1893). Nematodes, mostly Australian and Fijian. Sydney, Australia, F. Cunninghame & Co., pp.  252-308.

CohnE.SherS.A. (1972). A contribution to the taxonomy of the genus Xiphinema Cobb, 1913. Journal of Nematology 4, 36-65.

CoomansA.HuysR.HeynsJ.LucM. (2001). Character analysis, phylogeny, and biogeography of the genus Xiphinema Cobb, 1913 (Nematoda: Longidoridae). Annales du Musée Royal de l’Afrique Centrale (Zoologie), Tervuren, Belgique 287, 1-239.

DecraemerW.RobbinsR.T. (2007). The who, what and where of Longidoridae and Trichodoridae. Journal of Nematology 39, 295-297.

De JesusD.S.de OliveiraC.M.G.GastauerM.AlexanderC. (2015). Longidorids from Minas Gerais State, Brazil, with focus on the morphometric variability of Xiphinema krugi (Nematoda: Longidoridae) populations. Tropical Plant Pathology 40, 88-101. DOI: 10.1007/s40858-015-0012-1

De LeyP.FélixM.A.FrisseL.M.NadlerS.A.SternbergP.W.ThomasW.K. (1999). Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology 1, 591-612. DOI: 10.1163/156854199508559

GermaniG. (1990). Description of Dolichodorus pellegrinensis sp. n. (Nematoda: Dolichodoridae) and Xiphinema fagesi sp. n. (Nematoda: Dorylaimidae) from New Caledonia. Nematologica 36, 73-80. DOI: 10.1163/002925990X00040

GoodeyT. (1936). A new dorylaimid nematode, Xiphinema radicicola n. sp. Journal of Helminthology 14, 69-72. DOI: 10.1017/S0022149X00030510

GozelU.LambertiF.AgostinelliA.RossoL.NguyenK.AdamsB. (2006). Molecular and morphological consilience in the characterisation and delimitation of five nematode species from Florida belonging to the Xiphinema americanum-group. Nematology 8, 521-532. DOI: 10.1163/156854106778613958

Gutiérrez-GutiérrezC.Palomares-RiusJ.E.Cantalapiedra-NavarreteC.LandaB.B.EsmenjaudD.CastilloP. (2010). Molecular analysis and comparative morphology to resolve a complex of cryptic Xiphinema species. Zoologica Scripta 39, 483-498. DOI: 10.1111/j.1463-6409.2010.00437.x

Gutiérrez-GutiérrezC.Cantalapiedra-NavarreteC.DecraemerW.VovlasN.PriorT.Palomares-RiusJ.E.CastilloP. (2012). Phylogeny, diversity, and species delimitation in some species of the Xiphinema americanum-group complex (Nematoda: Longidoridae), as inferred from nuclear and mitochondrial DNA sequences and morphology. European Journal of Plant Pathology 134, 561-597. DOI: 10.1007/s10658-012-0039-9

Gutiérrez-GutiérrezC.Cantalapiedra-NavarreteC.RemesalE.Palomares-RiusJ.E.Navas-CortésJ.A.CastilloP. (2013). New insight into the identification and molecular phylogeny of dagger nematodes of the genus Xiphinema (Nematoda: Longidoridae) with description of two new species. Zoological Journal of the Linnean Society 169, 548-579. DOI: 10.1111/zoj.12071

HandooZ.A.CartaL.K.SkantarA.M.SubbotinS.A.FraedrichS.S. (2016). Molecular and morphological characterization of a Xiphinema chambersi population from live oak trees in Jekyll Island, Georgia, with a re-description of the species and comments on its morphometric variations. Journal of Nematology 48, 20-27. DOI: 10.21307/jofnem-2017-005

HeY.SubbotinS.A.RubtsovaT.V.LambertiF.BrownD.J.F.MoensM. (2005). A molecular phylogenetic approach to Longidoridae (Nematoda: Dorylaimida). Nematology 7, 111-124. DOI: 10.1163/1568541054192108

JanaA.BaqriQ.H. (1977). Nematodes from West Bengal (India) 3. Five ectoparasitic nematode species of Longidoridae (Dorylaimida). Zoological Survey of India Newsletter 3, 353-354.

JenkinsW.R. (1964). A rapid centrifugal-flotation method for separating nematodes from soil. Plant Disease Reporter 48, 692.

LambertiF.JatalaP.AgostinelliA. (1987). A report of some Xiphinema species occurring in Peru. Nematologia Mediterranea 15, 103-109.

LambertiF.De LucaF.MolinariS.DuncanL.W.AgostinelliA.CoiroM.I.DunnD.RadicciV. (2002). Xiphinema chambersi and Xiphinema naturale sp. n., two monodelphic longidorids (Nematoda, Dorylaimida) from Florida. Nematologica Mediterranea 30, 3-10.

LoofP.A.A.LucM. (1990). A revised polytomous key for the identification of species of the genus Xiphinema Cobb, 1913 (Nematoda: Longidoridae) with exclusion of the X. americanum-group. Systematic Parasitology 16, 35-66. DOI: 10.1007/BF00009600

LoofP.A.A.LucM. (1993). A revised polytomous key for the identification of species of the genus Xiphinema Cobb, 1913 (Nematoda: Longidoridae) with exclusion of the X. americanum-group: supplement 1. Systematic Parasitology 24, 185-189. DOI: 10.1007/BF00010531

LoofP.A.A.LucM.BaujardP. (1996). A revised polytomous key for the identification of species of the genus Xiphinema Cobb, 1913 (Nematoda: Longidoridae) with exclusion of the X. americanum-group: supplement 2. Systematic Parasitology 33, 23-29. DOI: 10.1007/BF00009717

LoofP.A.A.CoomansA.BaujardP.LucM. (2001). On five species of the genus Xiphinema Cobb, 1913 (Nematoda: Longidoridae) recently described from India. Nematology 3, 277-283. DOI: 10.1163/156854101750413351

LoosC.A. (1949). Notes on free-living and plant-parasitic nematodes of Ceylon. No. 5. Journal of the Zoological Society of India 1, 23-29.

LordelloL.G.E. (1951). Xiphinema brasiliense, nova espécie de nematoide do Brasil, parasita de Solanum tuberosum L. Bragantia 11, 87-90. DOI: 10.1590/S0006-87051951000100010

LucM. (1958). Xiphinema of west Africa: description of five new species (Nematoda: Dorylamidae). Nematologica 3, 57-72. DOI: 10.1163/187529258X00346

LucM.CoomansA. (1992). Plant parasitic nematodes of the genus Xiphinema (Longidoridae) in Guiana and Martinique. Belgian Journal of Zoology 122, 147-183.

MaraisM.SwartA.HeynsJ. (1995). A new species and a new record of Dorylaimida (De Man) Pearse (Nemata) from the Amazonas Province, Brazil. African Plant Protection 1, 19-24.

OliveiraC.M.BrownD.J.F.NeilsonR.MonteiroA.R.FerrazL.C.C.LambertiF. (2003). The occurrence and geographic distribution of Xiphinema and Xiphidorus species (Nematoda: Longidoridae) in Brazil. Helminthologia 40, 41-54.

OlsonM.HarrisT.HigginsR.MullinP.PowersK.OlsonS.PowersT.O. (2017). Species delimitation and description of Mesocriconema nebraskense n. sp. (Nematoda: Criconematidae), a morphologically cryptic, parthenogenetic species from North American grasslands. Journal of Nematology 49, 42-66. DOI: 10.21307/jofnem-2017-045

Palomares-RiusJ.E.Cantalapiedra-NavarreteC.Archidona-YusteA.SubbotinS.A.CastilloP. (2017). The utility of mtDNA and rDNA for barcoding and phylogeny of plant-parasitic nematodes from Longidoridae (Nematoda, Enoplea). Scientific Reports 7, 10905. DOI: 10.1038/s41598-017-11085-4

Peraza-PadillaW.Archidona-YusteA.FerrisH.Zamora-ArayaT.Cantalapiedra-NavarreteC.Palomares-RiusJ.E.SubbotinS.A.CastilloP. (2017). Molecular characterization of pseudomonodelphic dagger nematodes of the genus Xiphinema Cobb, 1913 (Nematoda: Longidoridae) in Costa Rica, with notes on Xiphinema setariae Tarjan, 1964. European Journal of Plant Pathology 148, 739-747. DOI: 10.1007/s10658-016-1124-2

PhukanP.N.SanwalK.C. (1982). Taxonomic studies on six species of Xiphinema from Assam. Journal of Research, Assam Agricultural University 3, 76-83.

Rahman-RazakA.LoofP.A.A. (1998). The genus Xiphinema Cobb, 1913 (Nematoda: Longidoridae) in western Malaysia. Fundamental and Applied Nematology 21, 413-428.

RobbinsR.T.BrownD.J.F. (1991). Comments on the taxonomy, occurrence and distribution of Longidoridae (Nematoda) in North America. Nematologica 37, 395-419. DOI: 10.1163/187529291X00402

RonquistF.HuelsenbeckJ.P. (2003). MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572-1574. DOI: 10.1093/bioinformatics/btg180

Roshan-BakhshA.PourjamE.PedramM.RobbinsR.T.DecraemerW. (2014). Description of Xiphinema castilloi sp. n. (Dorylaimida: Longidoridae) from Iran and its phylogenetic relationships. Nematology 16, 359-368. DOI: 10.1163/15685411-00002770

Schuurmans StekhovenJ.H.TeunissenR.J.H. (1938). Nématodes libres terrestres. Exploration du Parc National Albert: Mission G. F. de Witte (1933-1935) 22, pp.  1-229.

SeinhorstJ.W. (1959). A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4, 67-69. DOI: 10.1163/187529259X00381

SenD.ChatterjeeA.MannaB. (2010). A new and a known species of Xiphinema Cobb, 1913 (Dorylaimida: Xiphinematidae) from West Bengal, India with a key to the mono-opisthodelphic species of the genus. Nematologia Mediterranea 38, 187-195.

SilvaR.A.SilvaE.S.AntedomenicoS.R.InomotoM.M. (2008). Fauna of phytonematodes in the Atlantic forest from Ribeira Valley, São Paulo State, Brazil. Nematropica 38, 1-12.

Tanha MaafiZ.SubbotinS.A.MoensM. (2003). Molecular identification of cyst-forming nematodes (Heteroderidae) from Iran and a phylogeny based on the ITS sequences of rDNA. Nematology 5, 99-111. DOI: 10.1163/156854102765216731

ThompsonJ.D.GibsonT.J.JeanmouginF.HigginsD.G. (1997). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 4876-4882. DOI: 10.1093/nar/25.24.4876

ThorneG. (1937). Notes on free living and plant parasitic nematodes. III. Proceedings of the Helminthological society of Washington 4, 16-18.

ThorneG. (1939). A monograph of the nematodes of the superfamily Dorylaimoidea. Capita Zoologica 8, 1-261.

TzortzakakisE.A.Archidona-YusteA.Cantalapiedra-NavarreteC.NasiouE.LazanakiM.S.KabourakisE.M.Palomares-RiusJ.E.CastilloP. (2014). Integrative diagnosis and molecular phylogeny of dagger and needle nematodes of olives and grapevines in the island of Crete, Greece, with description of Xiphinema cretense n. sp. (Nematoda, Longidoridae). European Journal of Plant Pathology 3, 563-590. DOI: 10.1007/s10658-014-0488-4

VrainT.C.WakarchukD.A.LevesqueA.C.HamiltonR.I. (1992). Intraspecific rDNA restriction fragment length polymorphisms in the Xiphinema americanum group. Fundamental and Applied Nematology 15, 563-573.

WangS.WuX. (1992). [ Two species of Xiphinema Cobb, 1913 (Dorylaimida, Longidoridae) from around grape roots in China.] Acta Phytopathologica Sinica 22, 117-123.

YeW. (2002). Morphological and molecular taxonomy of Longidorus and Xiphinema (Nematoda: Longidoridae) occurring in Arkansas, USA. Ph.D. Dissertation, University of Arkansas, Fayetteville, Arkansas, USA.

YeW.Giblin-DavisR.M.BraaschH.MorrisK.ThomasW.K. (2007). Phylogenetic relationships among Bursaphelenchus species (Nematoda: Parasitaphelenchidae) inferred from nuclear ribosomal and mitochondrial DNA sequence data. Molecular Phylogenetics and Evolution 43, 1185-1197. DOI: 10.1016/j.ympev.2007.02.006

YuQ.BadissA.ZhangZ.YeW. (2010). First report and morphological, molecular characterization of Xiphinema chambersi Thorne, 1939 (Nematoda, Longidoridae) in Canada. ZooKeys 49, 13-22. DOI: 10.3897/zookeys.49.473

ZengY.YeW.ZhangZ.SunH.YongL.HuangY.ZhaoK.LiangH.KernsJ. (2016). Morphological and molecular characterization of Xiphinema species from Shenzhen, China. Helminthologica 53, 62-75. DOI: 10.1515/helmin-2015-0068

ZhengJ.SubbotinS.A.HeS.GuJ.MoensM. (2003). Molecular characterisation of some Asian isolates of Bursaphelenchus xylophilus and B. mucronatus using PCR-RFLPs and sequences of ribosomal DNA. Russian Journal of Nematology 11, 17-22.

Figures

  • Xiphinema hangzhouense sp. n. A: Pharynx; B: Gonad; C: Female tail region; D: Lip region; E-H: Tail region of first-, second-, third- and fourth-stage juveniles. (Scale bars: A, E-H = 500 μm; B-D = 10 μm.)

    View in gallery
  • Xiphinema hangzhouense sp. n. A: Entire female body (arrow showing position of vulva); B: Anterior region (arrow showing vulval position); C: Lip region (arrow showing position of guide ring); D, E: Lip region; F: Pharyngo-intestinal junction showing cardia; G: Vulval region in ventral view; H: Vulval region in lateral view; I, J: Pharyngeal bulb arrows showing dorsal and ventrosublateral nuclei; K: Female posterior region showing egg; L, M: Female tail arrow showing position of anus. Abbreviations: a = anus; gr = guide ring; dn = dorsal gland nucleus; svn = subventrolateral nuclei; V = vulva. (Scale bars: A = 500; B = 100; C-I = 10; J-M = 50 μm.)

    View in gallery
  • Xiphinema hangzhouense sp. n. A-D: Lip region of first-, second-, third- and fourth-stage juveniles, respectively. (Scale bars = 10 μm.)

    View in gallery
  • Xiphinema hangzhouense sp. n. A-D: Pharyngeal region of first-, second-, third- and fourth-stage juveniles, respectively; E-H: Tail region of first-, second-, third- and fourth-stage juveniles, respectively (arrow showing anus). (Scale bars = 50 μm.)

    View in gallery
  • Phylogenetic relationships of Xiphinema hangzhouense sp. n. with related Xiphinema species as inferred from Bayesian analysis using the 18S rRNA gene sequence dataset under the GTR + I + G model. Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

    View in gallery
  • Phylogenetic relationships of Xiphinema hangzhouense sp. n. with related Xiphinema species as inferred from Bayesian analysis using the D2-D3 of 28S rRNA gene sequence dataset under the GTR + I + G model. Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold. * Identified as X. vulgare by Gozel et al. (2006).

    View in gallery
  • Phylogenetic relationships of Xiphinema hangzhouense sp. n. with related Xiphinema species as inferred from Bayesian analysis using the ITS rRNA gene sequence dataset under the GTR + I + G model. Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

    View in gallery
  • Phylogenetic relationships of Xiphinema hangzhouense sp. n. with related Xiphinema species as inferred from Bayesian analysis using partial COI gene sequence dataset under the GTR + I + G model. Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

    View in gallery

Information

Content Metrics

Content Metrics

All Time Past Year Past 30 Days
Abstract Views 8 8 8
Full Text Views 6 6 6
PDF Downloads 0 0 0
EPUB Downloads 0 0 0