Bursaphelenchus decraemerae n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from the USA

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Bursaphelenchus decraemerae n. sp., isolated in Ningbo, China, from packaging wood made of Pinus sp. imported from the USA, is described. It is characterised by the lateral field with three lines, a stylet 10-12 μm long with small basal swellings, excretory pore located 4-12 μm posterior to median bulb, spicule small, ventrally arcuate with lamina gradually tapering to distal tip, rostrum triangular pointed to conical, condylus elongated with bluntly rounded tip, and cucullus minute, barely visible. There are seven caudal papillae arranged as a single ventral precloacal papilla, one adcloacal pair and two postcloacal pairs. The bursal flap is narrow and oval. A small vulval flap is present in lateral view of the female and the tail is conical, strongly ventrally curved with the terminus finely pointed and sometimes appearing like a mucron. The new species is characterised by morphological and molecular analysis and is close to B. gerberae and B. sakishimanus.


International Journal of Fundamental and Applied Nematological Research



BraaschH.SchönfeldU.PolomskiJ.BurgermeisterW. (2004). Bursaphelenchus vallesianus sp. n. – a new species of the Bursaphelenchus sexdentati group (Nematoda: Parasitaphelenchidae). Nematologia Mediterranea 32, 71-79.

BraaschH.BurgermeisterW.GuJ. (2009). Revised intrageneric grouping of Bursaphelenchus Fuchs, 1937 (Nematoda: Aphelenchoididae). Journal of Nematode Morphology and Systematics 12, 65-88.

BraaschH.GuJ.WangJ.WangJ.BurgermeisterW. (2012). A subspecies of Bursaphelenchus corneolus Massey, 1966 (Nematoda: Aphelenchoididae) and synonymization of B. curvicaudatus Wang, Yu & Lin, 2005 with B. corneolus. Journal of Nematode Morphology and Systematics 15, 53-67.

BurgermeisterW.MetgeK.BraaschH.BuchbachE. (2005). ITS-RFLP patterns for differentiation of 26 Bursaphelenchus species (Nematoda: Parasitaphelenchidae) and observations on their distribution. Russian Journal of Nematology 13, 29-42.

BurgermeisterW.BraaschH.MetgeK.GuJ.SchroderT.WoldtE. (2009). ITS-RFLP analysis, an efficient tool for differentiation of Bursaphelenchus species. Nematology 11, 649-668. DOI: 10.1163/156854108/399182

DarribaD.TaboadaG.L.DoalloR.PosadaD. (2012). jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9, 772. DOI: 10.1038/nmeth.2109

De GrisseA.T. (1969). Redescription ou modifications de quelques techniques utilisées dans l’étude de nématodes phytoparasitaires. Medelelingen Rijksfaculteit Landbouwwetenschappen Gent 34, 315-359.

De LeyP.FélixM.A.FrisseL.M.NadlerS.A.SternbergP.W.ThomasW.K. (1999). Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology 1, 591-612. DOI: 10.1163/156854199508559

FAO (Food and Agriculture Organization of the United Nations) (2003). Guidelines for regulating wood packaging material in international trade. International standards for phytosanitary measures (ISPM), No. 15. Rome, Italy, FAO.

FerrisV.R.FerrisJ.M.FaghihiJ. (1993). Variation in spacer ribosomal DNA in some cyst forming species of plant parasitic nematodes. Fundamental and Applied Nematology 16, 177-184.

FutaiK. (2013). Pine wood nematode, Bursaphelenchus xylophilus. Annual Review of Phytopathology 51, 61-83. DOI: 10.1146/annurev-phyto-081211-172910

Giblin-DavisR.M.KanzakiN.YeW.CenterB.J.ThomasW.K. (2006). Morphology and systematic of Bursaphelenchus gerberae n. sp. (Nematoda: Parasitaphelenchidae), a rare associate of the palm weevil, Rhynchophorus palmarum in Trinidad. Zootaxa 1189, 39-53.

GuJ.WangJ.DuanW.BraaschH.BurgermeisterW.ZhengJ. (2010). Description of Bursaphelenchus paraparvispicularis n. sp. (Nematoda: Aphelenchoididae) found in packaging wood from Hong Kong, China. Nematology 12, 557-566. DOI: 10.1163/138855409X12584413195455

GuJ.HeJ.WangJ.ChenX. (2014). Description of Bursaphelenchus yuyaoensis n. sp. (Nematoda: Aphelenchoididae) isolated from Pinus massoniana in China. Nematology 16, 411-418. DOI: 10.1163/15685411-00002773

GuJ.TomalakM.HeJ.FangY. (2017a). Bursaphelenchus crenati Rühm, 1956 (Tylenchina: Aphelenchoididae), a nematode associated with the Greater ash bark beetle, Hylesinus crenatus Fabricius, in dying ash, Fraxinus excelsior L., in Europe. Nematology 19, 413-426. DOI: 10.1163/15685411-00003058

GuJ.KanzakiN.TomalakM. (2017b). Proposal of Bursaphelenchus wuae n. comb. and its two junior synonyms. Nematology 19, 869-870. DOI: 10.1163/15685411-00003091

HoltermanM.van der WurffA.van den ElsenS.van MegenH.HolovachovO.BakkerJ.HelderJ. (2006). Phylum wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Molecular Biology and Evolution 23, 1792-1800. DOI: 10.1093/molbev/msl044

HuangR.E.YeJ.R. (2006). Seinura lii n. sp. and S. wuae n. sp. (Nematoda: Seinuridae) from pine wood in China. Nematology 8, 749-759. DOI: 10.1163/156854106778877848

KanzakiN. (2008). Taxonomy and systematics of the nematode genus Bursaphelenchus (Nematoda: Parasitaphelenchidae). In: ZhaoB.G.FutaiK.SutherlandJ.R.TakeuchiY. (Eds). Pine wilt disease. Tokyo, Japan, Springer. DOI: 10.1007/978-4-431-75655-2_6

KanzakiN.AkibaM.KanetaniS.TetsukaK.IkegameH. (2014). Bursaphelenchus osumiana n. sp. (Tylenchomorpha: Aphelenchoididae) isolated from dead Pinus armandii var. amamiana in Osumi Islands in Japan. Nematology 16, 903-916. DOI: 10.1163/15685411-00002817

KanzakiN.OkabeK.KoboriY. (2015). Bursaphelenchus sakishimanus n. sp. (Tylenchomorpha: Aphelenchoididae) isolated from a stag beetle, Dorcus titanus sakishimanus nomura (Coleoptera: Lucanidae), on Ishigaki Island, Japan. Nematology 17, 531-542. DOI: 10.1163/15685411-00002887

KatohK.StandleyD.M. (2013). MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30, 772-780. DOI: 10.1093/molbev/mst010

LargetB.SimonD.L. (1999). Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution 16, 750-759. DOI: 10.1093/oxfordjournals.molbev.a026160

LarssonA. (2014). AliView: a fast and lightweight alignment viewer and editor for large datasets. Bioinformatics 30, 3276-3278. DOI: 10.1093/bioinformatics/btu531

LiH.TrinhP.Q.WaeyenbergeL.MoensM. (2008). Bursaphelenchus chengi sp. n. (Nematoda: Parasitaphelenchidae) isolated at Nanjing, China, in packaging wood from Taiwan. Nematology 10, 335-346. DOI: 10.1163/156854108783900294

MariaM.FangY.GuJ.LiH. (2016). Redescription of Bursaphelenchus parapinasteri (Tylenchina: Aphelenchoididae) isolated from Pinus thunbergii in China with a key to the hofmanni-group. Nematology 18, 933-947. DOI: 10.1163/15685411-00003006

RonquistF.HuelsenbeckJ.P. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572-1574. DOI: 10.1093/bioinformatics/btg180

RyssA.VieiraP.MotaM.KulinichO. (2005). A synopsis of the genus Bursaphelenchus Fuchs, 1937 (Aphelenchida: Parasitaphelenchidae) with keys to species. Nematology 7, 393-458. DOI: 10.1163/156854105774355581

RyssA.PolyaninaK.S.PopovichevB.G.SubbotinS.A. (2015). Description of Bursaphelenchus ulmophilus sp. n. (Nematoda: Parasitaphelenchinae) associated with Dutch elm disease of Ulmus glabra huds. in the Russian north west. Nematology 17, 685-703. DOI: 10.1163/15685411-00002902

SeinhorstJ.W. (1959). A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4, 67-69. DOI: 10.1163/187529259X00381

StöverB.C.MüllerK.F. (2010). TreeGraph 2: combining and visualizing evidence from different phylogenetic analyses. BMC Bioinformatics 11, 7. DOI: 10.1186/1471-2105-11-7

VrainT.C. (1993). Restriction fragment length polymorphism separates species of the Xiphinema americanum group. Journal of Nematology 25, 361-364.

WangH.Y.ZhangS.S. (2007). [ Description of Bursaphelenchus parapinasteri sp. nov. (Nematoda, Parasitaphelenchidae) in wood of Pinus massoniana from Fujian province, China.] Acta Zootaxonomica Sinica/Dongwu Fenlei Xuebao 32, 906-912.

YeW.Giblin-DavisR.M. (2013). Molecular characterization and development of real-time PCR assay for pine-wood nematode Bursaphelenchus xylophilus (Nematoda: Parasitaphelenchidae). PLoS ONE 8, e78804. DOI: 10.1371/journal.pone.0078804

YeW.Giblin-DavisR.M.BraaschH.MorrisK.ThomasW.K. (2007). Phylogenetic relationships among Bursaphelenchus species (Nematoda: Parasitaphelenchidae) inferred from nuclear ribosomal and mitochondrial DNA sequence data. Molecular Phylogenetics and Evolution 43, 1185-1197. DOI: 10.1016/j.ympev.2007.02.006


  • Bursaphelenchus decraemerae n. sp. A: Entire female; B: Entire Male; C: Anterior head region; D: Anterior body showing lateral lines; E: Female gonad; F: Male tail in ventral view; G: Male tail in lateral view; H, I: Spicule; J, K: Female tail. (Scale bars = 10 μm.)

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  • Light photomicrographs of Bursaphelenchus decraemerae n. sp. male. A: Entire body; B: Anterior region showing excretory pore and hemizonid; C-E: Tail in lateral view showing position of papillae. (Abbreviations: ex = excretory pore; h = hemizonid; P + number = genital papillae.) (Scale bars = 10 μm.)

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  • Light photomicrographs of Bursaphelenchus decraemerae n. sp. female. A: Entire body; B: Anterior region; C: Gonad region; D: Lateral lines; E-G: Female tail. (Scale bars = 10 μm.)

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  • Phylogenetic relationships of Bursaphelenchus decraemerae n. sp. and aphelenchid nematodes based on a concatenated dataset (partial 18S rDNA + full length of ITS rDNA + partial 28S D2-D3 rDNA). The 10001st Bayesian tree was inferred under the GTR + I + G model. Aphelenchoides besseyi served as the outgroup species. Posterior probability values exceeding 50% are given on appropriate clades.

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