Bursaphelenchus decraemerae n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from the USA

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Bursaphelenchus decraemerae n. sp., isolated in Ningbo, China, from packaging wood made of Pinus sp. imported from the USA, is described. It is characterised by the lateral field with three lines, a stylet 10-12 μm long with small basal swellings, excretory pore located 4-12 μm posterior to median bulb, spicule small, ventrally arcuate with lamina gradually tapering to distal tip, rostrum triangular pointed to conical, condylus elongated with bluntly rounded tip, and cucullus minute, barely visible. There are seven caudal papillae arranged as a single ventral precloacal papilla, one adcloacal pair and two postcloacal pairs. The bursal flap is narrow and oval. A small vulval flap is present in lateral view of the female and the tail is conical, strongly ventrally curved with the terminus finely pointed and sometimes appearing like a mucron. The new species is characterised by morphological and molecular analysis and is close to B. gerberae and B. sakishimanus.

Nematology

International Journal of Fundamental and Applied Nematological Research

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References

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Figures

  • Bursaphelenchus decraemerae n. sp. A: Entire female; B: Entire Male; C: Anterior head region; D: Anterior body showing lateral lines; E: Female gonad; F: Male tail in ventral view; G: Male tail in lateral view; H, I: Spicule; J, K: Female tail. (Scale bars = 10 μm.)

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  • Light photomicrographs of Bursaphelenchus decraemerae n. sp. male. A: Entire body; B: Anterior region showing excretory pore and hemizonid; C-E: Tail in lateral view showing position of papillae. (Abbreviations: ex = excretory pore; h = hemizonid; P + number = genital papillae.) (Scale bars = 10 μm.)

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  • Light photomicrographs of Bursaphelenchus decraemerae n. sp. female. A: Entire body; B: Anterior region; C: Gonad region; D: Lateral lines; E-G: Female tail. (Scale bars = 10 μm.)

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  • Phylogenetic relationships of Bursaphelenchus decraemerae n. sp. and aphelenchid nematodes based on a concatenated dataset (partial 18S rDNA + full length of ITS rDNA + partial 28S D2-D3 rDNA). The 10001st Bayesian tree was inferred under the GTR + I + G model. Aphelenchoides besseyi served as the outgroup species. Posterior probability values exceeding 50% are given on appropriate clades.

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