Bursaphelenchus moensi n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from the USA

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Bursaphelenchus moensi n. sp., isolated in Tianjin Port, China, from Pinus packaging wood imported from the USA, is described. It is characterised by a lateral field with three lines (two bands), stylet 13.0-14.9 μm long, excretory pore situated at same level as, or slightly posterior to, the nerve ring, spicules 13.0-15.5 μm long, mitten-shaped, condylus high and broad with squared, rounded or pointed end, rostrum triangular or conical with bluntly rounded tip, and cucullus absent, bursa small, starting posterior to P4, narrow oblong to irregular in shape and with an oval central projection, female vulval flap absent, and tail conical with a finely rounded or mucronate terminus. The new species belongs to the hofmanni-group and is most similar to B. paraparvispicularis, B. parvispicularis and B. wuae. It is distinguished from closely related species by morphology, ITS-RFLP patterns and partial 18S, ITS and 28S D2-D3 rDNA gene sequencing results.

Nematology

International Journal of Fundamental and Applied Nematological Research

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References

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Figures

  • Bursaphelenchus moensi n. sp. A: Entire female; B: Entire Male; C: Anterior body region; D: Female gonad; E: Lateral lines; F: Male tail in ventral view; G: Male tail in lateral view; H: Spicules; I: Male tail termini; J, K: Female tail. (Scale bars = 10 μm.)

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  • Light photomicrographs of Bursaphelenchus moensi n. sp. male. A: Entire body; B: Anterior body region; C: Tail in ventral view showing position of papillae; D-G: Tail terminus; H-K: Tail in lateral view showing position of papillae. (P + number = genital papillae.) (Scale bars = 10 μm.)

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  • Light photomicrographs of Bursaphelenchus moensi n. sp. female. A: Entire body; B: Gonad region; C, D: Anterior region showing excretory pore and hemizonid; E, F: Vulval region; G: Lateral lines (arrows); H-J: Female tail. (Abbreviations: ex = excretory pore; h = hemizonid.) (Scale bars = 10 μm.)

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  • Scanning electron microscopy of Bursaphelenchus moensi n. sp. A, B: Female tail; C, D: Male tail showing papillae arrangement. (Scale bars = 10 μm.)

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  • ITS-RFLP patterns of Bursaphelenchus moensi n. sp. Restriction fragments were obtained by digestion of the amplified rDNA fragment (1) with RsaI (2), HaeIII (3), MspI (4), HinfI (5) and AluI (6). M = DNA marker (100 bp ladder, Invitrogen Life Technologies).

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  • Phylogenetic relationships of Bursaphelenchus moensi n. sp. and aphelenchid nematodes based on a concatenated dataset (partial 18S rDNA + full-length ITS rDNA + partial 28S D2-D3 rDNA). The 10 001st Bayesian tree was inferred under the GTR + I + G model, Aphelenchoides besseyi served as outgroup species. Posterior probability values exceeding 50% are given on appropriate clades.

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