Bursaphelenchus moensi n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from the USA

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Bursaphelenchus moensi n. sp., isolated in Tianjin Port, China, from Pinus packaging wood imported from the USA, is described. It is characterised by a lateral field with three lines (two bands), stylet 13.0-14.9 μm long, excretory pore situated at same level as, or slightly posterior to, the nerve ring, spicules 13.0-15.5 μm long, mitten-shaped, condylus high and broad with squared, rounded or pointed end, rostrum triangular or conical with bluntly rounded tip, and cucullus absent, bursa small, starting posterior to P4, narrow oblong to irregular in shape and with an oval central projection, female vulval flap absent, and tail conical with a finely rounded or mucronate terminus. The new species belongs to the hofmanni-group and is most similar to B. paraparvispicularis, B. parvispicularis and B. wuae. It is distinguished from closely related species by morphology, ITS-RFLP patterns and partial 18S, ITS and 28S D2-D3 rDNA gene sequencing results.


International Journal of Fundamental and Applied Nematological Research



AkbulutS.BraaschH.BaysalI.BrandstetterM.BurgermeisterW. (2007). Description of Bursaphelenchus anamurius sp. n. (Nematoda: Parasitaphelenchidae) from Pinus brutia in Turkey. Nematology 9, 859-867. DOI: 10.1163/156854107782331207

BaujardP. (1980). Trois nouvelles espèces de Bursaphelenchus (Nematoda: Tylenchida) et remarques sur le genre. Revue de Nématologie 3, 167-177.

BraaschH. (1998). Bursaphelenchus hofmanni sp. n. (Nematoda, Aphelenchoididae) from spruce wood in Germany. Nematologica 44, 615-621. DOI: 10.1163/005725998X00023

BraaschH.BurgermeisterW.GuJ. (2009). Revised intra-generic grouping of Bursaphelenchus Fuchs, 1937 (Nematoda: Aphelenchoididae). Journal of Nematode Morphology and Systematics 12, 65-88.

BraaschH.GuJ.WangJ.WangJ.BurgermeisterW. (2012). A subspecies of Bursaphelenchus corneolus Massey, 1966 (Nematoda: Aphelenchoididae) and synonymization of B. curvicaudatus Wang, Yu & Lin, 2005 with B. corneolus. Journal of Nematode Morphology and Systematics 15, 53-67.

BurgermeisterW.BraaschH.MetgeK.GuJ.SchroderT.WoldtE. (2009). ITS-RFLP analysis, an efficient tool for differentiation of Bursaphelenchus species. Journal of Nematology 11, 649-668. DOI: 10.1163/156854108/399182

DarribaD.TaboadaG.L.DoalloR.PosadaD. (2012). jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9, 772. DOI: 10.1038/nmeth.2109

De GrisseA.T. (1969). Redescription ou modifications de quelques techniques utilisées dans l’étude de nématodes phytoparasitaires. Medelelingen Rijksfaculteit Landbouwwetenschappen Gent 34, 315-359.

De LeyP.FélixM.A.FrisseL.M.NadlerS.A.SternbergP.W.ThomasW.K. (1999). Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology 1, 591-612. DOI: 10.1163/156854199508559

FAO (Food and Agriculture Organization of the United Nations) (2003). Guidelines for regulating wood packaging material in international trade. International Standards for Phytosanitary Measures (ISPM), No. 15. Rome, Italy, FAO.

FerrisV.R.FerrisJ.M.FaghihiJ. (1993). Variation in spacer ribosomal DNA in some cyst forming species of plant parasitic nematodes. Fundamental and Applied Nematology 16, 177-184.

Giblin-DavisR.M.KanzakiN.YeW.CenterB.J.ThomasW.K. (2006). Morphology and systematics of Bursaphelenchus gerberae n. sp. (Nematoda: Parasitaphelenchidae), a rare associate of the palm weevil, Rhynchophorus palmarum in Trinidad. Zootaxa 1189, 39-53.

GuJ.WangJ.DuanW.BraaschH.BurgermeisterW.ZhengJ. (2010). Description of Bursaphelenchus paraparvispicularis n. sp. (Nematoda: Aphelenchoididae) found in packaging wood from Hong Kong, China. Nematology 12, 557-566. DOI: 10.1163/138855409X12584413195455

GuJ.HeJ.WangJ.ChenX. (2014). Description of Bursaphelenchus yuyaoensis n. sp. (Nematoda: Aphelenchoididae) isolated from Pinus massoniana in China. Nematology 16, 411-418. DOI: 10.1163/15685411-00002773

GuJ.KanzakiN.TomalakM. (2017). Proposal of Bursaphelenchus wuae n. comb. and its two junior synonyms. Nematology 19, 869-870. DOI: 10.1163/15685411-00003091

HoltermanM.van der WurffA.van den ElsenS.van MegenH.HolovachovO.BakkerJ.HelderJ. (2006). Phylum wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Molecular Biology and Evolution 23, 1792-1800. DOI: 10.1093/molbev/msl044

HuangR.E.YeJ.R. (2006). Seinura lii n. sp. and S. wuae n. sp. (Nematoda: Seinuridae) from pine wood in China. Nematology 8, 749-759. DOI: 10.1163/156854106778877848

KanzakiN.FutaiK. (2005). Description of Bursaphelenchus parvispicularis n. sp. (Nematoda: Parasitaphelenchidae) isolated from a dead oak tree, Quercus mongolica var. grosseserrata. Nematology 7, 751-759. DOI: 10.1163/156854105775142964

KanzakiN.AkibaM.KanetaniS.TetsukaK.IkegameH. (2014). Bursaphelenchus osumiana n. sp. (Tylenchomorpha: Aphelenchoididae) isolated from dead Pinus armandii var. amamiana in Osumi Islands in Japan. Nematology 16, 903-916. DOI: 10.1163/15685411-00002817

KanzakiN.OkabeK.KoboriY. (2015). Bursaphelenchus sakishimanus n. sp. (Tylenchomorpha: Aphelenchoididae) isolated from a stag beetle, Dorcus titanus sakishimanus Nomura (Coleoptera: Lucanidae), on Ishigaki Island, Japan. Nematology 17, 531-542. DOI: 10.1163/15685411-00002887

KatohK.StandleyD.M. (2013). MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30, 772-780. DOI: 10.1093/molbev/mst010

LargetB.SimonD.L. (1999). Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution 16, 750-759. DOI: 10.1093/oxfordjournals.molbev.a026160

LarssonA. (2014). AliView: a fast and lightweight alignment viewer and editor for large datasets. Bioinformatics 30, 3276-3278. DOI: 10.1093/bioinformatics/btu531

LiH.TrinhP.Q.WaeyenbergeL.MoensM. (2008). Bursaphelenchus chengi sp. n. (Nematoda: Parasitaphelenchidae) isolated at Nanjing, China, in packaging wood from Taiwan. Nematology 10, 335-346. DOI: 10.1163/156854108783900294

MariaM.FangY.GuJ.LiH. (2016). Redescription of Bursaphelenchus parapinasteri (Tylenchina: Aphelenchoididae) isolated from Pinus thunbergii in China with a key to the hofmanni-group. Nematology 18, 933-947. DOI: 10.1163/15685411-00003006

PedramM.PourjamE.YeW.AtighiM.R.RobbinsR.T.RyssA. (2011). Description of Bursaphelenchus mazandaranense sp. n. (Nematoda: Parasitaphelenchidae) from Iran. Russian Journal of Nematology 19, 121-129.

RonquistF.HuelsenbeckJ.P. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572-1574. DOI: 10.1093/bioinformatics/btg180

RyssA.PolyaninaK.S.PopovichevB.G.SubbotinS.A. (2015). Description of Bursaphelenchus ulmophilus sp. n. (Nematoda: Parasitaphelenchinae) associated with Dutch elm disease of Ulmus glabra Huds. in the Russian north west. Nematology 17, 685-703. DOI: 10.1163/15685411-00002902

SeinhorstJ.W. (1959). A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4, 67-69. DOI: 10.1163/187529259X00381

StöverB.C.MüllerK.F. (2010). TreeGraph 2: combining and visualizing evidence from different phylogenetic analyses. BMC Bioinformatics 11, 7. DOI: 10.1186/1471-2105-11-7

VrainT.C. (1993). Restriction fragment length polymorphism separates species of the Xiphinema americanum group. Journal of Nematology 25, 361-364.

WangH.Y.ZhangS.S. (2007). [ Description of Bursaphelenchus parapinasteri sp. nov. (Nematoda, Parasitaphelenchidae) in wood of Pinus massoniana from Fujian province, China.] Acta Zootaxonomica Sinica/Dongwu Fenlei Xuebao 32, 906-912.

WangX.GuJ.MariaM.FangY.LiH. (2018). Bursaphelenchus decraemerae n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from the USA. Nematology 20, 119-131. DOI: 10.1163/15685411-00003129

YeW.Giblin-DavisR.M.BraaschH.MorrisK.ThomasW.K. (2007). Phylogenetic relationships among Bursaphelenchus species (Nematoda: Parasitaphelenchidae) inferred from nuclear ribosomal and mitochondrial DNA sequence data. Molecular Phylogenetics and Evolution 43, 1185-1197. DOI: 10.1016/j.ympev.2007.02.006


  • Bursaphelenchus moensi n. sp. A: Entire female; B: Entire Male; C: Anterior body region; D: Female gonad; E: Lateral lines; F: Male tail in ventral view; G: Male tail in lateral view; H: Spicules; I: Male tail termini; J, K: Female tail. (Scale bars = 10 μm.)

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  • Light photomicrographs of Bursaphelenchus moensi n. sp. male. A: Entire body; B: Anterior body region; C: Tail in ventral view showing position of papillae; D-G: Tail terminus; H-K: Tail in lateral view showing position of papillae. (P + number = genital papillae.) (Scale bars = 10 μm.)

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  • Light photomicrographs of Bursaphelenchus moensi n. sp. female. A: Entire body; B: Gonad region; C, D: Anterior region showing excretory pore and hemizonid; E, F: Vulval region; G: Lateral lines (arrows); H-J: Female tail. (Abbreviations: ex = excretory pore; h = hemizonid.) (Scale bars = 10 μm.)

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  • Scanning electron microscopy of Bursaphelenchus moensi n. sp. A, B: Female tail; C, D: Male tail showing papillae arrangement. (Scale bars = 10 μm.)

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  • ITS-RFLP patterns of Bursaphelenchus moensi n. sp. Restriction fragments were obtained by digestion of the amplified rDNA fragment (1) with RsaI (2), HaeIII (3), MspI (4), HinfI (5) and AluI (6). M = DNA marker (100 bp ladder, Invitrogen Life Technologies).

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  • Phylogenetic relationships of Bursaphelenchus moensi n. sp. and aphelenchid nematodes based on a concatenated dataset (partial 18S rDNA + full-length ITS rDNA + partial 28S D2-D3 rDNA). The 10 001st Bayesian tree was inferred under the GTR + I + G model, Aphelenchoides besseyi served as outgroup species. Posterior probability values exceeding 50% are given on appropriate clades.

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