Description of Myolaimus mycophilus Slos & Bert sp. n. (Rhabditida: Myolaimidae)

in Nematology
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Myolaimus mycophilus Slos & Bert sp. n. was isolated from mushrooms from different locations in Belgium and cultured on nutrient agar seeded with Escherichia coli OP50. Myolaimus mycophilus sp. n. is characterised by a unique combination of the following characters: adults with lateral fields not differentiated, buccal cavity triangular, cheilostom anisomorphic, female with a post-vulval uterine sac as long as the corresponding body diam., wrinkled eggshell, male with seven pairs of genital papillae, cloacal aperture covered by a trapezoidal cloacal flap and with a single ventral papilla at its base. The new species and M. goodeyorum Andrássy, 1984 were molecularly characterised by sequencing the 18S and D2-D3 28S expansion segments of rDNA. Phylogenetic relationships were reconstructed based on concatenated analyses and the phylogenetic placement of Myolaimus, an intriguing genus within the Rhabditida, is discussed.

Description of Myolaimus mycophilus Slos & Bert sp. n. (Rhabditida: Myolaimidae)

in Nematology



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    Line drawings of Myolaimus mycophilus Slos & Bert sp. n. A: Female, schematic overview; B: Male, schematic overview; C: Male, anterior region; D: Female, tail region; E: Male tail, lateral view; F: Male tail, ventral view.

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    Light micrographs of Myolaimus mycophilus Slos & Bert sp. n. A. Male, lateral view anterior region; B: Female tail; C: Lateral view female reproductive system; D: Ventral view male tail; E: Lateral view male tail; F: Egg.

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    Scanning electron micrographs of Myolaimus mycophilus Slos & Bert sp. n. A, B: Anterior end of female; C: Male en face; D: Female vulva; E: Female vulva with mating plug; F: Female anus, detail; G: Female anus and tail region; H, I: Male bursa with genital papillae, respectively ventral and ventro-lateral view; J: Detail of cloacal region; K, L: Detail of male tail, respectively dorsal and posterior dorsal. (Arrowheads = pre-cloacal papillae; asterisk = precloacal flap.)

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    Phylogenetic tree of Myolaimus in Rhabditida inferred from MrBayes Bayesian phylogenetic analysis (GTR + I + G) from combined 28S and 18S rDNA genes with two outgroup species. Branch support is indicated in following order: PP value in BI analysis/BS value from ML analysis. The classification system follows De Ley & Blaxter (2002).

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