Morphological and molecular characterisation of Aphelenchoides primadentus n. sp. (Nematoda: Aphelenchoididae) from northern Iran

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Aphelenchoides primadentus n. sp. is described and illustrated based on its morphological, morphometric and molecular characters. It was recovered from natural forests of northern Iran in association with mosses. The new species is characterised by its body length of 502-613 μm in females and 461-564 μm in males, presence of three lines in the lateral fields, a low lip region slightly offset by a shallow depression, 11.1-13.8 μm long stylet in females, its conus being longer than the shaft (m = 53.0-68.5) and bearing well-developed, rounded basal knobs, the excretory pore located at ca one body diam. posterior to the metacorpus, a conical tail with a multipapillate terminus or with a single, warty, wide mucron-like structure at the tip, and males with 19-22 μm long arcuate spicules that lack a well-developed condylus and rostrum. The new species was morphologically compared with species of the genus having three lines in the lateral field and a multipapillate tail tip or tail with warty mucron, namely A. iranicus and A. heidelbergi. Compared to A. ensete, A. huntensis and A. gorganensis, three species with a multipapillate tail tip or warty mucron at the tail tip, the new species has a basic difference in the number of lines in the lateral field and, compared to two species, A. composticola and A. petersi, which have three lines in the lateral field and a similar overall morphology, the new species has basic differences in tail tip morphology. The results of phylogenetic analyses using partial sequences of ribosomal small subunit RNA (SSU) and mitochondrial cytochrome c oxidase 1 (COI mtDNA) genes revealed there is currently no sequenced species of the genus phylogenetically close to the new species being sequenced for these two genomic and non-genomic regions. The new species forms a clade with A. ritzemabosi in SSU, and three other species, A. besseyi, A. ritzemabosi and A. fujianensis, in the COI tree.

Nematology

International Journal of Fundamental and Applied Nematological Research

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Figures

  • Line drawings of Aphelenchoides primadentus n. sp. A: Pharynx; B, C: Entire body of female and male (arrowheads pointing to caudal papillae); D, E: Details of vagina; F: Details of anterior end; G: Spicules; H: Posterior body region of female.

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  • Light microphotographs of Aphelenchoides primadentus n. sp. A-D: Details of anterior end; E: Part of female reproductive system; F: Part of pharynx (arrowhead showing excretory pore); G, I: Cross sections of female and male, respectively (mid-body); H: Spicule; J: Postvulval uterine sac (arrowhead pointing at tip); K-O: Tail (K: Female; N: Arrowheads showing P2-P4 caudal papillae). (Scale bars = 10 μm.)

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  • Light microphotographs of Aphelenchoides primadentus n. sp. A-D: Details of anterior end, showing longer conus of stylet compared to shaft and rounded knobs; E-H: Variation in tail end morphology (E-G: Multipapillate tail end, G: Male, H: Wide warty mucron-like structure at tail end). (Scale bars = 10 μm.)

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  • Bayesian tree inferred under the GTR + G + I model using 18S rDNA sequence of Aphelenchoides primadentus n. sp. Posterior probability and bootstrap values exceeding 50% are given on appropriate clades in the form BPP/BS. The new species is in bold font.

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  • Bayesian tree inferred under the GTR + G + I model using COI mtDNA sequence of Aphelenchoides primadentus n. sp. Posterior probability and bootstrap values exceeding 50% are given on appropriate clades in the form BPP/BS. The new species is in bold font.

    View in gallery

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