Morphological and molecular characterisation of Heterodera filipjevi (Madzhidov, 1981) from the Slovak Republic

in Nematology
Restricted Access
Get Access to Full Text
Rent on DeepDyve

Have an Access Token?



Enter your access token to activate and access content online.

Please login and go to your personal user account to enter your access token.



Help

Have Institutional Access?



Access content through your institution. Any other coaching guidance?



Connect

Cysts, males and juveniles from a population of Heterodera filipjevi found on sports-ground turf in Trnava, Slovak Republic, in 2016 are described based on morphology and morphometrics. The identity of species of juveniles extracted from cysts was subsequently determined and confirmed by PCR-RFLP and sequencing. Morphologically, cysts, juveniles and males from the Slovak Republic are similar to paratypes from Tajikistan. The results of the phylogenetic analysis of the ITS rRNA gene sequences confirmed the species identification and phylogenetic relationship of H. filipjevi with other Heterodera species. Of interest, this analysis showed the close similarity between the Slovakian (KY349106) and Chinese (KU896216) samples, which differed by a single nucleotide and clustered together. To our knowledge, this is also the first report of H. filipjevi from the Slovak Republic, thus increasing the total number of cyst nematode species of the Heterodera genus known to occur in the country to a total of 15 species.

Nematology

International Journal of Fundamental and Applied Nematological Research

Sections

References

BishnoiS.P. (2009). Importance of cereal cyst nematodes in Rajasthan, India and its control through breeding resistance. In: Riley, I.T., Nicol, J.M. & Dababat, A.A. (Eds). Cereal cyst nematodes: status, research and outlook. Proceedings of the first workshop of the international cereal cyst nematode initiative, Antalya, Turkey, 21-23 October 2009. Ankara, Turkey, CIMMYT, pp. 143-148.

BrzeskiM.W. (1998). Nematodes of Tylenchina in Poland and temperate Europe. Warsaw, Poland, Muzeum I Instytut Zoologii PAN.

CobbN.A. (1918). Estimating the nema population of the soil, with special reference to the sugar-beet and root-gall nemas, Heterodera schachtii Schmidt and Heterodera radicicola (Greef) Muller, and with a description of Tylencholaimus aequalis n. sp. Agricultural Technical Circular of US Department of Agriculture 1, 1-47.

De GrisseA. (1969). Redescription ou modifications de quelques techniques utilisées dans l’étude des nématodes phytoparasitaires. Mededelingen Rijksfaculteit der Landbouwwetenschappen Gent 34, 351-369.

EvansK.RoweJ.A. (1998). Distribution and economic importance. In: SharmaS.B. (Ed.). The cyst nematodes. Dordrecht, The Netherlands, Kluwer Academic Publishers, pp.  1-30.

FelsensteinJ. (1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783-791. DOI: 10.1111/j.1558-5646.1985.tb00420.x

FerrisV.R.SubbotinS.A.IreholmA.SpiegelY.FaghihiJ.FerrisJ.M. (1999). Ribosomal DNA sequence analysis of Heterodera filipjevi and H. latipons isolates from Russia and comparisons with other nematode isolates. Russian Journal of Nematology 7, 121-125.

FranklinM.T. (1969). Heterodera latipons n. sp., a cereal cyst nematode from the Mediterranean region. Nematologica 15, 535-542. DOI: 10.1163/187529269X00867

HajihasaniA.Tanha MaafiZ.NicolJ.M.RezaeeS. (2010). Effect of the cereal cyst nematode, Heterodera filipjevi, on wheat in microplot trials. Nematology 12, 357-363. DOI: 10.1163/138855409X12548945788321

HandooZ.A. (2002). A key and compendium to species of the Heterodera avenae group (Nematoda: Heteroderidae). Journal of Nematology 34, 250-262.

HolgadoR.RoweJ.A.MagnussonC. (2004). Morphology of cysts and second stage juveniles of “Heterodera filipjevi” (Madzhidov, 1981) Stelter, 1984 from Norway. Journal of Nematode Morphology and Systematics 7, 77-84.

JoyceS.A.ReidA.DriverF.CurranJ. (1994). Application of polymerase chain reaction (PCR) methods to identification of entomopathogenic nematodes. In: Burnell, A.M., Ehlers, R.-U. & Masson, J.P. (Eds). Cost 812 Biotechnology: genetics of entomopathogenic nematode-bacterium complexes. Proceedings of symposium and workshop, St Patrick’s College, Maynooth, Ireland, Luxembourg, European Commission, DG XII, pp. 178-187.

KangH.EunG.HaJ.KimY.ParkN.KimD.ChoiI. (2016). New cyst nematode, Heterodera sojae n. sp. (Nematoda: Heteroderidae) from soybean in Korea. Journal of Nematology 48, 280-289.

KrallE.SturhanD.MüürJ. (1999). Cyst nematodes attacking cereals and grasses in Estonia. Transactions of the Estonian Agricultural University, Agronomy 203, 44-48.

LiH.L.YuanH.X.SunJ.W.FuB.NianG.L.HouX.S.SunB.J. (2010). First record of the cereal cyst nematode Heterodera filipjevi in China. Plant Disease 94, 1505. DOI: 10.1094/PDIS-04-10-0301

MadaniM.VovlasN.CastilloP.SubbotinS.A.MoensM. (2004). Molecular characterization of cyst nematode species (Heterodera spp.) from the Mediterranean basin using RFLPs and sequences of ITS-rDNA. Journal of Phytopathology 152, 229-234. DOI: 10.1111/j.1439-0434.2004.00835.x

MadzhidovA.R. (1981). [ New species of Bidera filipjevi sp. nov. (Heteroderina: Tylenchida) from Tadzhikistan.] Izvestiya Akademii Nauk Tadzhikskoi SSR Otdelenie Biologicheskikh Nauk 2, 40-44.

MadzhidovA.R. (1985). [ Description of the male of Bidera filipjevi Madzihov, 1981.] Izvestiya Akademii Nauk Tadzhikskoi Nauk 98, 86-88.

MadzhidovA.R. (1991). The cyst forming nematodes of the family Heteroderidae and their significance for the cereal crops of Tajikistan. Ph.D. Thesis, Moscow, Russia.

MitchinsonS.GowenS.R.KerryB.R. (2009). Increased biodiversity in cereal cyst nematode infestations is not a threat to intensive cereal production in southern Britain. In: Riley, I.T., Nicol, J.M. & Dababat, A.A. (Eds). Cereal cyst nematodes: status, research and outlook. Proceedings of the first workshop of the international cereal cyst nematode initiative, Antalya, Turkey, 21-23 October 2009. Ankara, Turkey, CIMMYT, pp. 215-220.

NeiM.KumarS. (2000). Molecular evolution and phylogenetics. New York, NY, USA, Oxford University Press.

NicolJ.M.BolatN.SahinE.TülekA.YildirimA.F.YorgancilarA.KaplanA.BraunH.J. (2005). The cereal cyst nematode is causing economic damage on rained wheat production systems of Turkey. In: Joint Meeting of the Annual Western Soil Fungus Conference and the American Phytopathalogy Society Pacific Division, Portland, United States of America, 28 June-1 July. [Abstr.]

PariyarS.R.DababatA.A.SiddiqueS.Erginbas-OrakciG.ElashryA.MorgounovA.GrundlerF.M. (2016). Identification and characterisation of resistance to the cereal cyst nematode Heterodera filipjevi in winter wheat. Nematology 18, 377-402. DOI: 10.1163/15685411-00002964

RenčoM. (2002). Occurrence of Heterodera schachtii and Heterodera avenae in the sugar beet growing areas of the Slovak Republic. Agriculture 48, 573-579.

RenčoM. (2005). Current occurrence and geographical distribution of Heterodera avenae Wollenweber 1924, in the Slovak Republic. Plant Protection Science 41, 80-85.

RivoalR.CookR. (1993). Nematode pests of cereals. In: EvansK.TrudgillD.L.WebsterJ.M. (Eds). Plant parasitic nematodes in temperate agriculture. Wallingford, UK, CABI Publishing, pp.  259-303.

RumpenhorstH.J.ElekçiogluI.H.SturhanD.ÖztörkG.EnneliS. (1996). The cereal cyst nematode Heterodera filipjevi (Madzhidov) in Turkey. Nematologia Mediterranea 24, 135-138.

SabováM.ValockáB.ZachaV.VančekE.KubenkováE. (1977). Rozšírenie hád’atka ovseného Heterodera avenae Wollenweber, 1924 v SSR. Agriculture 23, 1069-1075.

SabováM.ValockáB.LiškováM. (1979). Species of corn crop nematodes and their seasonal dynamics. Helminthologia 16, 35-44.

SabováM.ValockáB.LiškováM. (1980). [Helminthocenosis of cereals.] Final report, Helminthologický ústav SAV, Košice.

SabováM.ValockáB.LiškováM. (1988a). Dynamika nálezov cýst hád’atka ovseného (Heterodera avenae Woll.) v monokultúrach obilnín a v osevných postupoch na lokalitách SSR. Agriculture 34, 723-729.

SabováM.ValockáB.LiškováM.VargováV. (1988b). The first finding of Heterodera latipons Franklin, 1969 on grass stands in Czechoslovakia. Helminthologia 25, 201-206.

ŠályA. (1983). Free-living nematodes in the Slovak Socialist Republic. Bratislava, Slovak Socialist Republic, VEDA, Slovak Academy of Sciences.

SmileyR.W.YanG.P.HandooZ.A. (2008). First record of the cyst nematode Heterodera filipjevi on wheat in Oregon. Plant Disease 92, 1136. DOI: 10.1094/PDIS-92-7-1136B

StelterH. (1984). Die Arten der Unter familie Heteroderinae (Nematoda: Heteroderidae) und ihre Verbreitung. Berlin, DDR, Akademie der Landwirtschaftswissenschaften der DDR.

SturhanD. (1996). Occurrence of Heterodera filipjevi (Madzhidov, 1981) Stelter, 1984 in Iran. Pakistan Journal of Nematology 14, 89-93.

SturhanD.KrallE. (2002). Heterodera iri Mathews, 1971 a junior synonym of H. ustinovi Kirjanova, 1969. Russian Journal of Nematology 10, 55-57.

SturhanD.LiškováM. (2004). Cyst nematodes in the Slovak Republic. Helminthologia 41, 217-219.

SturhanD.RumpenhorstH.J. (1996). Studies on the Heterodera avenae complex. Mitteilungen-Biologischen Bundesanstalt fur Land und Forstwirtschaft 317, 75-91.

SubbotinS.A.RumpenhorstH.J.SturhanD. (1996). Morphological and electrophoretic studies on populations of the Heterodera avenae complex from the former USSR. Russian Journal of Nematology 4, 29-38.

SubbotinS.A.SturhanD.RumpenhorstH.J.MoensM. (2003). Molecular and morphological characterisation of the Heterodera avenae species complex (Tylenchida: Heteroderidae). Nematology 5, 515-538. DOI: 10.1163/156854103322683247

SubbotinS.A.Mundo-OcampoM.BaldwinJ.G. (2010a). Systematics of cyst nematodes (Nematoda: Heteroderinae). Nematology Monographs and Perspectives 8A (Series editors: HuntD.J.PerryR.N.). Leiden, The Netherlands, Brill.

SubbotinS.A.Mundo-OcampoM.BaldwinJ.G. (2010b). Systematics of cyst nematodes (Nematoda: Heteroderinae). Nematology Monographs and Perspectives 8B (Series editors: HuntD.J.PerryR.N.). Leiden, The Netherlands, Brill.

TamuraK.StecherG.PetersonD.FilipskiA.KumarS. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30, 2725-2729. DOI: 10.1093/molbev/mst197

Tanha MaafiZ.SubbotinS.A.MoensM. (2003). Molecular identification of cyst-forming nematodes (Heteroderidae) from Iran and a phylogeny based on ITS-rDNA sequences. Nematology 5, 99-111. DOI: 10.1163/156854102765216731

Tanha MaafiZ.SturhanD.KheiriA.GeraertE. (2007). Species of the Heterodera avenae group (Nematoda: Heteroderidae) from Iran. Russian Journal of Nematology 15, 49-58.

ThompsonJ.D.GibsonT.J.PlewniakF.JeanmouginF.HigginsD.G. (1997). The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 4876-4882. DOI: 10.1093/nar/25.24.4876

ToumiF.WaeyenbergeL.ViaeneN.DababatA.NicolJ.M.OgbonnayaF.MoensM. (2013). Development of two species-specific primer sets to detect the cereal cyst nematodes Heterodera avenae and Heterodera filipjevi. European Journal of Plant Pathology 136, 613-624. DOI: 10.1007/s10658-013-0192-9

TurnerS.J.SubbotinS.A. (2013). Cyst nematodes. In: PerryR.N.MoensM. (Eds). Plant nematology, 2nd edition. Wallingford, UK, CAB International, pp.  109-143.

WollenweberH.W. (1924). Zur Kenntnis der Kartoffel-Heteroderen. Illustrierte Landwirtschaftliche Zeitung 44, 100-101.

YanG.P.SmileyR.W. (2010). Distinguishing Heterodera filipjevi and H. avenae using polymerase chain reaction-restriction fragment length polymorphism and cyst morphology. Phytopathology 100, 216-224. DOI: 10.1094/PHYTO-100-3-0216

ZhuoK.WangH.YeW.PengD.LiaoJ. (2013). Heterodera hainanensis n. sp. (Nematoda: Heteroderinae) from bamboo in Hainan Province, China – a new cyst nematode in the Afenestrata group. Nematology 15, 303-314. DOI: 10.1163/15685411-00002678

Figures

  • Light microscope photos of cysts and juveniles of Heterodera filipjevi. A: Cysts; B-E: Vulval area of cysts; F: Entire body of second-stage juvenile (J2); G: Head region and stylet of J2; H-I: Tail of J2. (Scale bars: A = 500 μm; B-E, G-I = 10 μm; F = 100 μm.)

    View in gallery
  • Light microscope photos of males of Heterodera filipjevi. A: Entire body of male; B, C: Head region; D: Excretory pore; E: Sperm; F: Tail region with spicule. (Scale bars: A = 100 μm; B-F = 10 μm.)

    View in gallery
  • PCR-ITS-RFLP profile for the cyst nematode Heterodera filipjevi (Madzhidov, 1981): M = 100 bp DNA ladder (CEB), 1: AluI; 2: AvaI; 3: Bsh1236I; 4: HaeIII; 5: CfoI; 6: MvaI; 7: PstI; 8: RsaI.

    View in gallery
  • Phylogenetic tree resulting from alignment of ITS-rRNA sequences. The tree was inferred by Maximum Likelihood method under the GTR + G model. Bootstrap values were obtained from 100 replicates. Values higher than 50% are presented. Original sequence is indicated by bold font.

    View in gallery

Information

Content Metrics

Content Metrics

All Time Past Year Past 30 Days
Abstract Views 2 2 2
Full Text Views 4 4 4
PDF Downloads 0 0 0
EPUB Downloads 0 0 0