Panagrellus levitatus sp. n. (Rhabditida: Panagrolaimidae), a nematode suppressing Drosophila melanogaster in laboratory cultures

in Nematology
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A new nematode species recovered from the laboratory culture of Drosophila melanogaster is described and illustrated. The mass reproduction of Panagrellus levitatus sp. n. in the fly culture occurred several times and resulted in a significant reduction of the fly population. Nematode outbreaks happened after the introduction of D. melanogaster to the culture from natural sources. The new species is morphologically similar to P. ulmi. Partial LSU rDNA and SSU rDNA sequences were obtained and subjected to phylogenetic analysis that demonstrated the affinity of the new species with Panagrellus sp. ‘MC2014’ from a red palm weevil. For the first time, the dauer juveniles of Panagrellus were described.

Nematology

International Journal of Fundamental and Applied Nematological Research

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References

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Figures

  • Panagrellus levitatus sp. n. A-H: Female; I-O: Male; P, Q: Dauer juvenile. A, Q: Entire worm; B, I, J: Head; C: Vulval region; D, H: Female tail; E, F: Vulva; G: Ovary flexure; K: Testis; L, P: Anterior region; M: Male tail; N: Spicules and gubernaculum; O: Spicule distal tip. Except for D (ventral), all in lateral position. (Scale bars in μm.)

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  • Panagrellus levitatus sp. n. SEM images. Male: A, D, G, J, K; Female: B, E, H; Dauer juvenile: C, F, I, L. A-C: Head, arrows indicating amphids; D: Tail, subventral; G: Cloacal region, ventral, arrows indicating median and circumcloacal papillae; J: Tail extremity with posteriormost pair of papillae; K: Tail region, sublateral, arrow indicating phasmid, papillal pairs numbered 2-6, S = single midventral papilla; E, F: Lateral field at mid-body; H: Vulva; I: Anterior body, arrows indicating excretory pore and lateral field; L: Tail, ventral. (Scale bars in μm.)

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  • Panagrellus levitatus sp. n. Light photo. A: Stoma of female; B: Female tail in ventral position showing phasmids (arrows); C: Spermatids in male testis (arrow); D: Female oviduct showing sperm cells (arrow); E, F: Variations in vagina shape. (Scale bars in μm.)

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  • Phylogenetic relationships of Panagrellus levitatus sp. n. Bayesian analysis, alignment length 623 bp; posterior probabilities are indicated near nodes; scale bar = expected changes per site.

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