Morphological and molecular characterisation of two new Hemicycliophora species (Tylenchida: Hemicycliophoridae) with a revision of the taxonomic status of some known species and a phylogeny of the genus

In: Nematology

Summary

Hemicycliophora presently contains 132 valid species of sheath nematodes. Within several samples obtained from surveys in Canada, South Africa, Spain and the USA, we distinguished six valid and six putative unidentified species by integrating the results of morphological and molecular analyses. Valid species included: H. californica, H. gracilis, H. parvana, H. poranga, H. raskii, and H. signata. The putative unidentified species were indicated as Hemicycliophora sp. 10, sp. 12, sp. 15, sp. 16, sp. 17, and sp. 18. Two new species of sheath nematodes from Spain and the USA were described and named as H. onubensis sp. n. and H. robbinsi sp. n., respectively. Hemicycliophora wyei is proposed as a junior synonym of H. parvana and H. ripa is proposed as a junior synonym of H. poranga. Eighteen valid and 13 unidentified species of sheath nematodes were characterised using the partial COI mtDNA gene. A total of 94 new sequences of which 77 were for the COI mtDNA gene were obtained in this study. Phylogenetic relationships within Hemicycliophora, using the D2-D3 expansion segments of 28S rDNA, ITS rRNA and COI gene sequences, are presented as inferred from Bayesian analysis.

  • Abolafia, J., Liébanas, G. & Peña-Santiago, R. (2002). Nematodes of the order Rhabditida from Andalucía Oriental, Spain. The subgenus Pseudacrobeles Steiner, 1938, with description of a new species. Journal of Nematode Morphology and Systematics 4, 137-154.

    • Search Google Scholar
    • Export Citation
  • Bajaj, H.K. (1998). Criconematids of Kalesar forests and Morni hills of Haryana, India. Indian Journal of Nematology 28, 150-158.

  • Brzeski, M.W. (1974). Taxonomy of the Hemicycliophorinae (Nematoda, Tylenchida). Problemowe Postepow Nauk Rolniczych 154, 237-330.

  • Castillo, P., Gomez-Barcina, A. & Loof, P.A.A. (1989). On two species of Hemicycliophora de Man, 1921 (Nematoda: Criconematoidea) found in Spain. Nematologia Mediterranea 17, 77-82.

    • Search Google Scholar
    • Export Citation
  • Castillo, P., Vovlas, N., Subbotin, S. & Troccoli, A. (2003). A new root-knot nematode, Meloidogyne baetica n. sp. (Nematoda: Heteroderidae), parasitizing wild olive in southern Spain. Phytopathology 93, 1093-1102. DOI: 10.1094/PHYTO.2003.93.9.1093

    • Search Google Scholar
    • Export Citation
  • Chaves, E. (1983). Criconematoidea (Nematoda) from Argentina. Nematologica 29, 404-424. DOI: 10.1163/187529283X00285

  • Chitambar, J.J. & Subbotin, S.A. (2014). Systematics of the sheath nematodes of the superfamily Hemicycliophoroidea. Nematology Monographs and Perspectives 10 (Series Editors: Hunt, D.J. & Perry, R.N.). Leiden, The Netherlands, Brill.

    • Search Google Scholar
    • Export Citation
  • Colbran, R.C. (1960). Studies of plant and soil nematodes. 3. Belonolaimus hastulatus, Psilenchus tumidus and Hemicycliophora labiata, three new species from Queensland. Queensland Journal of Agricultural Science 17, 175-181.

    • Search Google Scholar
    • Export Citation
  • Coolen, W.A. (1979). Methods for extraction of Meloidogyne spp. and other nematodes from roots and soil. In: Lamberti, F. & Taylor, C.E. (Eds). Root-knot nematodes (Meloidogyne species). Systematics, biology, and control. London, UK, Academic Press, pp. 317-329.

    • Search Google Scholar
    • Export Citation
  • Cordero López, M.A., Robbins, R.T. & Szalanski, A.L. (2013). Taxonomic and molecular identification of Hemicycliophora, Caloosia, Gracilacus and Paratylenchus species (Nematoda: Criconematidae). Journal of Nematology 45, 145-171.

    • Search Google Scholar
    • Export Citation
  • Curran, J., Driver, F., Ballard, J.W.O. & Milner, R.J. (1994). Phylogeny of Metarhizium: analysis of ribosomal DNA sequence data. Mycological Research 98, 547-555. DOI: 10.1016/S0953-7562(09)80478-4

    • Search Google Scholar
    • Export Citation
  • De Grisse, A.T. (1969). Redescription ou modification de quelques techniques utilisées dans l’étude des nématodes phytoparasitaires. Mededelingen van de Rijksfaculteit der Landbouwwetenschappen Gent 34, 351-369.

    • Search Google Scholar
    • Export Citation
  • de Man, J.G. (1921). Nouvelles recherches sur les nématodes libres terricoles de la Hollande. Capita Zoologica 1, 3-62.

  • Doucet, M.E. (1982). Quatre nouvelles espèces du genre Hemicycliophora de Man, 1921 (Nematoda: Tylenchida) provenant d’Argentine. Revue de Nématologie 5, 309-320.

    • Search Google Scholar
    • Export Citation
  • Goffart, H. (1951). Nematoden aus unterirdischen Gewassern. Deutsche Zoologische Zeitschrift 1, 73-78.

  • Goodey, J.B. (1963). Soil and freshwater nematodes, 2nd edition. London, UK, Methuen.

  • Heyns, J. (1962). Two new species of Criconematidae from South Africa. Nematologica 8, 21-24. DOI: 10.1163/187529262X00963

  • Jairajpuri, M.S. & Baqri, Q.H. (1973). Nematodes of high altitudes in India. I. Four new species of Tylenchida. Nematologica 19, 19-30. DOI: 10.1163/187529273X00051

    • Search Google Scholar
    • Export Citation
  • Kirjanova, E.S. & Shagalina, L.M. (1974). [Hemicycliophora pruni sp. nov. (Nematoda: Hemicycliophoridae) – a parasite of plum tree in central Kopetdagh.] Izvestiya Akademii Nauk Turkmenskoi SSR, Serija Biologicheskikh Nauk 6, 35-39.

    • Search Google Scholar
    • Export Citation
  • Loof, P.A.A. (1968). Taxonomy of Hemicycliophora species from west and central Europe (Nematoda: Criconematoidea). Mededelingen Landbouwhogeschool Wageningen 68-14, 52 pp.

  • Loof, P.A.A. (1984). Hemicycliophora species from Iran (Nematoda: Criconematoidea). Nematologica 30, 22-41. DOI: 10.1163/187529284X00437

    • Search Google Scholar
    • Export Citation
  • Loof, P.A.A. & De Grisse, A. (1989). Taxonomic and nomenclatorial observations on the genus Criconemella De Grisse & Loof, 1965, sensu Luc & Raski, 1981. Mededelingen Faculteit Landbouwwetenschappen Rijksuniversiteit, Gent 54, 53-74.

    • Search Google Scholar
    • Export Citation
  • Loof, P.A.A. & Heyns, J. (1969). Taxonomy of Hemicycliophora species from South Africa (Nematoda: Criconematoidea). Nematologica 15, 464-472. DOI: 10.1163/187529269X00795

    • Search Google Scholar
    • Export Citation
  • Loos, C.A. (1948). Notes on free-living and plant-parasitic nematodes of Ceylon 3. Ceylon Journal of Science 23, 119-124.

  • Ma, X. & Agudelo, P. (2015). First report of Hemicycliophora wyei on bentgrass in Texas. Plant Disease 99, 732. DOI: 10.1094/PDIS-09-14-0972-PDN

    • Search Google Scholar
    • Export Citation
  • Mehta, U.K., Raski, D.J. & Valenzuela, A.A. (1983). Five new species of Criconematidae (Nemata) from southern Chile. Nematologica 28(1982), 398-411. DOI: 10.1163/187529282X00204

    • Search Google Scholar
    • Export Citation
  • Micoletzky, H. (1913). Die freilebenden Süsswassernematoden der Ost-Alpen. 1. Teil der vorlaüfi ge Mitteilung: Die frilebenden Süsswassernematoden des Lunzer Seengebietes. Sitzungsberichte der K. Akademie der Wissenschaften in Wien, Mathematisch-Naturwissenschaftliche-Klasse, Abt. 1 122(1), 111-122; 122(4), 543-548.

    • Search Google Scholar
    • Export Citation
  • Monteiro, A.R. & Lordello, L.G.E. (1978). A description of Hemicycliophora poranga n. sp. from Brazil (Nemata). Revista Brasileira de Biologia 38, 569-571.

    • Search Google Scholar
    • Export Citation
  • Netscher, C. & Seinhorst, J.W. (1969). Propionic acid better than acetic acid for killing nematodes. Nematologica 15, 286.

  • Orton Williams, K.J. (1978). Two new species of the genus Hemicycliophora de Man, 1921 (Nematoda: Tylenchida). Revue de Nématologie 1, 197-205.

    • Search Google Scholar
    • Export Citation
  • Powers, T.O., Bernard, E.C., Harris, T., Higgins, R., Olson, M., Lodema, M., Mullin, P., Sutton, L. & Powers, K.S. (2014). COI haplotype groups in Mesocriconema (Nematoda: Criconematidae) and their morphospecies associations. Zootaxa 3827, 101-146. DOI: 10.11646/zootaxa.3827.2.1

    • Search Google Scholar
    • Export Citation
  • Powers, T.O., Bernard, E.C., Harris, T., Higgins, R., Olson, M., Olson, S., Lodema, M., Matczyszyn, J., Mullin, P., Sutton, L. et al. (2016a). Species discovery and diversity in Lobocriconema (Criconematidae: Nematoda) and related plant-parasitic nematodes from North American ecoregions. Zootaxa 4085, 301-344. DOI: 10.11646/zootaxa.4085.3.1

    • Search Google Scholar
    • Export Citation
  • Powers, T.O., Mullin, P., Higgins, R., Harris, T. & Powers, K.S. (2016b). Description of Mesocriconema ericaceum n. sp. (Nematoda: Criconematidae) and notes on other nematode species discovered in an ericaceous heath bald community in Great Smoky Mountains National Park, USA. Nematology 18, 879-903. DOI: 10.1163/15685411-00003001

    • Search Google Scholar
    • Export Citation
  • Raski, D.J. (1958). Four new species of Hemicycliophora de Man, 1921, with further observations on H. brevis Thorne, 1955 (Nematoda: Criconematidae). Proceedings of the Helminthological Society of Washington 25, 125-131.

    • Search Google Scholar
    • Export Citation
  • Reed, J.P. & Jenkins, W.R. (1963). Hemicycliophora vaccinium n. sp. (Nematoda: Criconematidae) from cranberry. Proceedings of the Helminthological Society of Washington 30, 211-212.

    • Search Google Scholar
    • Export Citation
  • Ronquist, F. & Huelsenbeck, J.P. (2003). MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572-1574. DOI: 10.1093/bioinformatics/btg180

    • Search Google Scholar
    • Export Citation
  • Schneider, W. (1925). Freilebende Süsswassernematodes aus ostholsteinischen Seen. Nebst Bemerkungen über die Nematodenfauna des Madu und Schaalsees II. Archiv für Hydrobiologie 15, 536-310.

    • Search Google Scholar
    • Export Citation
  • Seinhorst, J.W. (1962). On the killing, fixation and transferring to glycerin of nematodes. Nematologica 8, 29-32. DOI: 10.1163/187529262X00981

    • Search Google Scholar
    • Export Citation
  • Seinhorst, J.W. (1966). Killing nematodes for taxonomic study with hot F.A. 4:1. Nematologica 12, 178. DOI: 10.1163/187529266X00239

  • Subbotin, S.A., Vovlas, N., Crozzoli, R., Sturhan, D., Lamberti, F., Moens, M. & Baldwin, J.G. (2005). Phylogeny of Criconematina Siddiqi, 1980 (Nematoda: Tylenchida) based on morphology and D2-D3 expansion segments of the 28S rRNA gene sequences with application of a secondary structure model. Nematology 7, 927-944. DOI: 10.1163/156854105776186307

    • Search Google Scholar
    • Export Citation
  • Subbotin, S.A., Sturhan, D., Chizhov, V.N., Vovlas, N. & Baldwin, J.G. (2006). Phylogenetic analysis of Tylenchida Thorne, 1949 as inferred from D2 and D3 expansion fragments of the 28S rRNA gene sequences. Nematology 8, 455-474. DOI: 10.1163/156854106778493420

    • Search Google Scholar
    • Export Citation
  • Subbotin, S.A., Chitambar, J.J., Chizhov, V.N., Stanley, J.D., Inserra, R.N., Doucet, M.E., McClure, M., Ye, W., Yeates, G.W., Mollov, D.S. et al. (2014). Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae). Zoological Journal of the Linnean Society 171, 475-506. DOI: 10.1111/zoj.12145

    • Search Google Scholar
    • Export Citation
  • Swofford, D.L. (2003). PAUP*: phylogenetic analysis using parsimony (*and other methods), version 4.0b 10. Sunderland, MA, USA, Sinauer Associates.

    • Search Google Scholar
    • Export Citation
  • Tanha Maafi, Z., Subbotin, S.A. & Moens, M. (2003). Molecular identification of cyst-forming nematodes (Heteroderidae) from Iran and a phylogeny based on ITS-rDNA sequences. Nematology 5, 99-111. DOI: 10.1163/156854102765216731

    • Search Google Scholar
    • Export Citation
  • Tarjan, A.C. (1952). The nematode genus Hemicycliophora de Man, 1921 (Criconematidae) with a description of a new plant-parasitic species. Proceedings of the Helminthological Society of Washington 19, 65-77.

    • Search Google Scholar
    • Export Citation
  • Taylor, A.L. (1936). The genera and species of the Criconematidae, a subfamily of the Anguillulinidae (Nematoda). Transactions of the American Microscopical Society 55, 391-421.

    • Search Google Scholar
    • Export Citation
  • Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 4876-4882.

    • Search Google Scholar
    • Export Citation
  • Thorne, G. (1955). Fifteen new species of the genus Hemicycliophora with an emended description of H. typica de Man (Tylenchida, Criconematidae). Proceedings of the Helminthological Society of Washington 22, 1-16.

    • Search Google Scholar
    • Export Citation
  • Van den Berg, E. (1981). Further studies on the genus Hemicycliophora de Man, 1921 in South Africa (Nematoda: Hemicycliophoroidea) with description of a new species. Phytophylactica 13, 181-194.

    • Search Google Scholar
    • Export Citation
  • Van den Berg, E. (1987a). New Hemicycliophora species from the Western Cape Province (Hemicycliophoridae: Nematoda). Phytophylactica 19, 65-70.

    • Search Google Scholar
    • Export Citation
  • Van den Berg, E. (1987b). More Hemicycliophora species from South Africa (Hemicycliophoridae: Nematoda). Phytophylactica 19, 309-314.

  • Van den Berg, E., Subbotin, S.A. & Tiedt, L.R. (2010). Morphological and molecular characterisation of Hemicycliophora lutosa Loof & Heyns, 1969 and H. typica, 1921 from South Africa (Nematoda: Hemicycliophoridae). Nematology 12, 303-308. DOI: 10.1163/138855409X12511010796671

    • Search Google Scholar
    • Export Citation
  • Van den Berg, E., Tiedt, L.R., Inserra, R.N., Stanley, J.D., Vovlas, N., Palomares-Rius, J.E., Castillo, P. & Subbotin, S.A. (2015). Characterisation of a topotype and other populations of Hemicriconemoides strictathecatus Esser, 1960 (Nematoda: Criconematidae) from Florida with description of H. phoenicis sp. n. from the USA. Nematology 17, 265-300. DOI: 10.1163/15685411-00002866

    • Search Google Scholar
    • Export Citation
  • Zeng, Y., Ye, W., Kerns, J., Tredway, L., Martin, S. & Martin, M. (2015). Molecular characterization and phylogenetic relationships of plant-parasitic nematodes associated with turf grasses in North Carolina and South Carolina, United States. Plant Disease 99, 982-993. DOI: 10.1094/PDIS-10-14-1060-RE

    • Search Google Scholar
    • Export Citation

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