The effect of storage time and temperature on the population dynamics and vitality of Meloidogyne chitwoodi in potato tubers

in Nematology
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The population densities of Meloidogyne chitwoodi in potato tubers stored at 4, 8 and 12°C after 0, 60, 120, 180 and 240 days of storage were assessed. Compared to day 0, storage temperatures of 4 and 8°C reduced population densities to 9 and 35%, respectively, after 240 days of storage, while nematode numbers in tubers stored at 12°C increased 2.5 times. The maximum hatching rate of nematodes from tubers stored at 8 and 12°C increased linearly with storage time. At 4°C it remained constant. The time required for the hatching process to reach the maximum number of second-stage juveniles (J2) decreased with increasing storage temperature. Recovered juveniles of M. chitwoodi from tubers after 180 and 240 days of storage at all three temperatures were still infective and able to multiply on ‘Desiree’ with estimates of the maximum multiplication rate (a) and the maximum population density (M) of 63.6 and 70.8 J2 (g dry soil)−1, respectively.

The effect of storage time and temperature on the population dynamics and vitality of Meloidogyne chitwoodi in potato tubers

in Nematology



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    Description of the list of variables and parameters used.

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    Experiment 1. The relation between relFTW and storage time ts, at 4, 8 and 12°C. Fitted model: Equation (1) relFTW=mFTW+(1mFTW)×kts. Solid line (mFTW) represents the minimum relative tuber weight.

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    Experiment 1: Parameter estimates of the logistic model, Equation (2), fitted to the cumulative hatched second-stage juveniles of Meloidogyne chitwoodi from the tuber peel for each storage time and temperature combination.

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    Experiment 1. The cumulative hatch of juveniles of Meloidogyne chitwoodi at 4, 8 and 12°C after 60, 120, 180 and 240 days of storage. The solid black line is the cumulative hatch immediately after harvest (or ts = 0). Fitted model: Equation (2) F(t)=C1+exp(B(tA)).

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    Experiment 1. The relation between the hatching parameters log C, B and A and storage time, ts, at 4, 8 and 12°C. The linear models, Equations (3) and (5), were fitted to the data. At 12°C the parameter A reached its minimum. Therefore, Equation (6) was fitted. The open circle (∘) represents the observation at harvest.

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    Experiment 1. Parameters estimates of the relations between the Meloidogyne chitwoodi hatching parameters C, B and A, and storage time ts. according to the log linear and linear Equations (4) and (5).

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    Experiment 2. Initial population densities,, and final population densities, Pfr+sor, of Meloidogyne chitwoodi at harvest of the pot test with potato ‘Desiree’.

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    Experiment 2. Relation between and Pfr+s of Meloidogyne chitwoodi on ‘Desiree’ in the viability test. The diagonal dotted line represents the equilibrium densities were Pi = Pf. The thick and thinner solid lines are according to Equation (7): Pf=(M·Pi)/(Pi+(M/a)), representing the 50, 2.5 and 97.5% quantiles.

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    Experiment 2. The cumulative hatch of juveniles of Meloidogyne chitwoodi from peel at harvest of the viability test. Initial population densities originated from tubers stored for 180 and 240 days at 4, 8 and 12°C in Experiment 1. Fitted model: Equation (2): F(t)=C1+exp(B(tA)).

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    Experiment 2. Relation between initial population densities (, and parameters C (, B and A of the logistic hatching curve of Meloidogyne chitwoodi on ‘Desiree’ in the viability test. Parameter C graph: the diagonal dotted line represents the equilibrium densities where Pi = Pf; the horizontal solid and broken lines indicate the maximum population densities, M, for Pi from tubers, after storage times, ts, of 180 and 240 days respectively. Parameter B and A graphs: the horizontal solid and broken lines indicate the nematodes originating from ts = 180 and 240 days, respectively.

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