Morphological and molecular characterisation of Hemicriconemoides paracamelliae sp. n. (Nematoda: Criconematidae) and two known species of Hemicriconemoides from China

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Summary

Sheathoid nematodes of the genus Hemicriconemoides are migratory root-ectoparasites of many crops but damage is documented for only a few species. Hemicriconemoides paracamelliae sp. n., isolated in Hangzhou, Zhejiang, China, from the rhizosphere of Cinninghamia lanceolata, is described together with H. kanayaensis and H. parataiwanensis. These three species are characterised morphologically and molecularly with important morphological details elucidated by SEM photographs. The new species can be characterised by the en face view having a dorsoventrally orientated oral disc with slit-like opening, and the labial plate being composed of two lateral semi-globular shaped projections on the lateral sides of the oral disc. This new species has the first lip annulus expanded and slightly wider than the second, stylet 83 (80-85) μm long, excretory pore located 5-6 annuli posterior to the base of the pharyngeal bulb, vulva slit-like, vulval flaps absent, spermatheca rounded to oblong-shaped, and anus located 5-8 annuli posterior to vulva. The tail is elongated, conoid, with the terminal annuli curving dorsally or ventrally to form a finely rounded tip. The study provides the first record of H. kanayaensis from mainland China, a new host association of H. parataiwanensis, and molecular sequencing data of the 18S, 28S D2-D3 and ITS sequences.

Morphological and molecular characterisation of Hemicriconemoides paracamelliae sp. n. (Nematoda: Criconematidae) and two known species of Hemicriconemoides from China

in Nematology

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Figures

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    Line drawings of Hemicriconemoides paracamelliae sp. n. Female. A: Pharyngeal region; B-D: Various lip regions; E: Posterior region showing reproductive system; F, G: En face view of lip region; H: SEM tail, ventral view; L, M: Posterior region showing vulva and anus; I-K: Dorsal and ventral curve of terminal tail annuli.

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    Light photomicrographs of Hemicriconemoides paracamelliae sp. n. Female. A: Entire body; B: Pharyngeal region, arrows showing position of base of pharyngeal bulb and excretory pore; C-E: Lip region; F-I: Posterior region, arrows showing position of vulva and anus. Abbreviations: a = anus; exp = excretory pore; phar. bulb = pharyngeal bulb; v = vulva. (Scale bars: A = 40 μm; B-I = 10 μm.)

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    Scanning electron micrographs of Hemicriconemoides paracamelliae sp. n. female. A-C: En face view of lip region; D: Cuticular annuli at mid-body; E-G: Posterior region showing vulva (v) and anus (a). (Scale bars: A, C = 10 μm; D, E-G = 20 μm; B = 5μm.)

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    Morphometrics of female Hemicriconemoides paracamelliae sp. n. All measurements are in μm and in the form: mean ± s.d. (range).

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    Light photomicrographs of Hemicriconemoides kanayaensis Nakasono & Ichinohe, 1961. Female. A: Entire body; B-D: Lip region; E: Pharyngeal region, arrows showing position of base of pharyngeal bulb and excretory pore; F-I: Posterior region, arrows showing position of vulva, anus and different tail terminus endings. Abbreviations: a = anus; exp. = excretory pore; phary. bulb = pharyngeal bulb; v = vulva. (Scale bars: A = 40 μm; B-I = 10 μm.)

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    Scanning electron micrographs of Hemicriconemoides kanayaensis Nakasono & Ichinohe, 1961. Female. A-D: En face view; E: Cuticular annuli at mid-body; F, G: Posterior region showing vulva (v) and anus (a), arrowed; H: Tail terminus showing anastomosis. (Scale bars: A = 4 μm; B = 5 μm; C, D = 10 μm; E = 50 μm; F = 30 μm; G, H = 20 μm.)

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    Morphometrics of Hemicriconemoides kanayaensis Nakasono & Ichinohe, 1961. female. All measurements are in μm and in the form: mean ± s.d. (range).

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    Light photomicrographs of Hemicriconemoides parataiwanensis Decraemer & Geraert, 1992. Female. A: Entire body; B: Pharyngeal region, arrows showing position of base of pharyngeal bulb and excretory pore; C-F: Different positions of lip region showing stylet projecting outside body and cuticular sheath pulled over lip region; G-J: Posterior region, arrows showing position of vulva, anus and variation in tail terminus. Abbreviations: a = anus; exp = excretory pore; phary. bulb = pharyngeal bulb; v = vulva. (Scale bars: A = 40 μm; B-J = 10 μm.)

  • View in gallery

    Scanning electron micrographs of Hemicriconemoides parataiwanensis Decraemer & Geraert, 1992. Female. A-C: En face view; D: Cuticular annuli at mid-body; E, F: Posterior region showing vulva and anus. (Scale bars: A, C = 5 μm; B = 10 μm; D, F = 20 μm; E = 30 μm.)

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    Morphometrics of Hemicriconemoides parataiwanensis Decraemer & Geraert, 1992. All measurements are in μm and in the form: mean ± s.d. (range).

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    Phylogenetic relationships within populations and species of Hemicriconemoides as inferred from Bayesian analysis using the D2-D3 of 28S rRNA gene sequence dataset with the GTR + I + G model. Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold. *Identified as H. strictathecatus by Subbotin et al. (2005) and Van den Berg et al. (2014); **identified as H. strictathecatus by Van den Berg et al. (2014).

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    Phylogenetic relationships within populations and species of Hemicriconemoides as inferred from Bayesian analysis using the ITS rRNA gene sequence dataset with the TVM + I + G model. Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold. *Identified as H. litchi by Chen et al. (2011) and H. strictathecatus by Van den Berg et al. (2014); **identified as H. strictathecatus by Van den Berg et al. (2014); ***identified as H. mangiferae by Chen et al. (2011); ****identified as H. californianus by Chen et al. (2011).

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    Phylogenetic relationships within populations and species of Hemicriconemoides as inferred from Bayesian analysis using the partial 18S rRNA gene sequence dataset with the TrN + G model. Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

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