First record of the genus Sakia Khan, 1964 (Nematoda: Tylenchidae) from Iran, with proposal of Sakia arboris n. sp.

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Summary

The genus Sakia is recorded from Iran for the first time and Sakia arboris n. sp., recovered from two geographical points in northern Iran, is described. The new species is characterised by its smooth cuticle under light microscopy (LM), absence of lateral fields, head continuous with body contour, flattened lip region, 9.3 (9.0-10.0) μm long stylet, vulva at 60.2 (59.3-61.3)%, bilobed spermatheca, 117 (102-128) μm long tail, and 14.3 (14-15) μm long spicules. By lacking lateral fields, the new species comes close to three known species of the genus, namely: S. alii, S. castori and S. indica. Molecular phylogenetic analyses using sequences of the near full-length fragment of the small subunit of ribosomal DNA (18S rDNA) and the D2-D3 expansion segments of the large subunit ribosomal DNA (28S rDNA) were performed using Bayesian inference (BI) and maximum likelihood (ML) methods. In the reconstructed Bayesian tree using the 18S rDNA sequence of the type population, the new species occupied a position in a clade including two isolates of Sakia sp. and some species of Lelenchus, with maximal BPP and high ML BS values (1.00/99%). In the reconstructed 28S rDNA phylogenetic tree, two newly sequenced isolates of S. arboris n. sp. formed a well-supported clade with Lelenchus spp.

Nematology

International Journal of Fundamental and Applied Nematological Research

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References

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Figures

  • Sakia arboris n. sp. A: Entire female; B: Entire male; C: Female pharyngeal region; D: Female reproductive system; E: Male spicules and bursa; F: Female pharyngeal bulb; G: Spermatheca; H: Female tail; I: Female stylet.

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  • Light microphotographs of Sakia arboris n. sp. A: Pharynx of female; B: Details of anterior end of female C: Reproductive system of female; D: Spermatheca and vulva; E, J: Tail tip of female; F: Part of pharynx showing metacorpus and pharyngeal bulb; G: Spermatheca; H: Cross section from mid-body; I: Spicules and bursa. (Scale bars = 10 μm.)

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  • Morphometrics of Sakia arboris n. sp. All measurements are in μm and in the form: mean ± s.d. (range).

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  • Bayesian 50% majority rule consensus tree inferred from 52 sequences of 18S rDNA under the GTR + G + I model. Bayesian posterior probabilities and maximum likelihood bootstrap values are given for appropriate clades in the shape BPP/ML BS. The new species is in bold.

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  • Bayesian 50% majority rule consensus tree inferred from 58 sequences of the D2-D3 domains of the 28S rDNA under the GTR + G + I model. Bayesian posterior probabilities and maximum likelihood bootstrap values are given for appropriate clades in the shape BPP/ML BS. The new species is in bold.

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  • Light microphotographs of amphidial opening and amphidial pouches in Sakia arboris n. sp. and two species of Lelenchus. A: Amphidial opening (arrowhead) in Sakia arboris n. sp.; B, C: Amphidial pouch (B, arrowhead) and amphidial opening (C, arrowhead) in Lelenchus leptosoma (Iranian population reported by Panahandeh et al., 2015a); D, E: Amphidial pouch (D, arrowhead) and amphidial opening (E, arrowhead) in L. brevislitus (paratype). (Scale bars = 10 μm.)

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