Paurodontella gilanica n. sp. (Nematoda: Sphaerularioidea) from Iran

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Summary

Paurodontella gilanica n. sp. is described and illustrated based on morphological, morphometric and molecular characters. The new species is characterised by its small body size, four lines in the lateral field, weak stylet with minute asymmetrical knobs, female reproductive system lacking a diverticulum but with small post-vulval uterine sac, elongate conoid tail with pointed, sometimes filiform, tip, males common with small spicules and cloacal bursa not reaching tail tip. Morphological differences between the new species and seven known species of the genus, namely P. asymmetrica, P. auriculata, P. balochistanica, P. minuta, P. myceliophaga, P. parapitica and P. persica, are discussed. The new species is also compared with four known species of Paurodontus having four lines in the lateral field. Using a 1123 nt long partial 18S rDNA sequence of the new species to reconstruct the phylogenetic relationships revealed that it formed a clade with members of the Sphaerulariidae and Paurodontidae. Using a 746 nt long partial sequence of the 28S rDNA D2-D3 segment revealed that P. gilanica n. sp. formed a clade with Abursanema iranicum in both Bayesian inference (BI) and maximum likelihood (ML) analyses with 0.99 Bayesian posterior probability (BPP) and 89% bootstrap support value (BS). The morphological affinities of Paurodontella and Paurodontus are discussed.

Nematology

International Journal of Fundamental and Applied Nematological Research

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References

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Figures

  • Line drawings of Paurodontella gilanica n. sp. A: Male entire body; B: Anterior body region with details of pharynx; C: Head region; D: Variation in pharynx extension into intestine; E: Female reproductive system; F: Stylet; G: Spicules and gubernaculum; H: Female entire body; I: Female tail; J: Male cloacal region.

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  • Photomicrographs of Paurodontella gilanica n. sp. A, B: Details of anterior end; C: Part of pharynx; D: Variation in extension of pharynx into intestine; E: Vulva to anus region; F, G: Cloacal region; H: Part of female reproductive system; I: Female tail. (Scale bars = 10 μm.)

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  • Morphometrics of Paurodontella gilanica n. sp. All measurements are in μm and in the form: mean ± s.d. (range).

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  • Consensus 50% majority rule Bayesian phylogenetic tree of Paurodontella gilanica n. sp. based on 18S rDNA sequences under GTR + I + G model. Bayesian posterior probabilities and maximum likelihood bootstrap values more than 50% are given on appropriate clades in the form BPP/ ML BS. The new sequence is in boldface.

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  • Consensus 50% majority rule Bayesian phylogenetic tree of Paurodontella gilanica n. sp. based on 28S D2-D3 rDNA sequences under GTR + G model. Bayesian posterior probabilities and maximum likelihood bootstrap values more than 50% are given on appropriate clades in the form BPP/ML BS. The new sequence is in boldface.

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  • Some useful morphological and morphometric data of species of the genera Paurodontus and Paurodontella.

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