Nicotiana benthamiana as model plant for Meloidogyne graminicola infection

in Nematology
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Nicotiana benthamiana is widely used as a model plant to analyse cell biology and to obtain insight into the molecular host-pathogen interaction because it is susceptible to many pathogens. Since N. benthamiana can be transformed easily, it is also used to study pathogens for which it is not a known host. Meloidogyne graminicola has a fairly broad host range of mainly monocots and some dicots but no data were available on the ability of M. graminicola to infect N. benthamiana. In this study, we show that M. graminicola is able to infect and complete its life cycle in N. benthamiana, although our experiments demonstrate a lower susceptibility compared to rice. In addition, M. graminicola was also able to develop in N. tabacum but the reproduction was very low. Therefore, we conclude that N. benthamiana can be considered as a host, while this is not the case for N. tabacum.

Nicotiana benthamiana as model plant for Meloidogyne graminicola infection

in Nematology



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    Galls (yellow arrowheads) induced by Meloidogyne graminicola on roots of Oryza sativa, Nicotiana benthamiana and N. tabacum. A: O. sativa (30 days after inoculation (dai)); B: N. benthamiana (30 dai); C: N. tabacum ‘Samsun-NN’ (30 dai). D: N. tabacum ‘Wisconsin 38’ (25 dai).

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    Fuchsin-stained galls of Meloidogyne graminicola in Oryza sativa, Nicotiana benthamiana and N. tabacum roots at 14 days after inoculation. A: Adult female in O. sativa with clearly visible giant cells; B: Adult females in O. sativa with egg mass; C: Adult female in N. benthamiana with clearly visible giant cells; D: Adult female in N. benthamiana with egg mass; E: Young nematode in N. tabacum ‘Samsun-NN’ with dissected feeding site; F: Adult female in N. tabacum ‘Samsun-NN’ with clearly visible giant cells and small egg mass; G: Young nematode in N. tabacum ‘Wisconsin 38’ with visible giant cells; H: Adult female in N. tabacum ‘Wisconsin 38’ with visible giant cells and egg mass. Asterisk = giant cells, n = nematode, e = egg mass.

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    Susceptibility of Oryza sativa, Nicotiana benthamiana and N. tabacum to Meloidogyne graminicola. Nematode infection was analysed at 14 days after inoculation on eight plants per group. A: Average number of galls and number of nematodes; B: Average number of females and egg-laying females. Bars represent the average number of galls or nematodes of eight infected plants. Significant difference is indicated by different letters (P<0.05 in Mann-Whitney U-test). Error bars represent the standard error of the mean; C: Percentages of developmental stages. To perform statistics percentage of separate stages were increased by different values (second-stage juveniles (J2) = 1; third- and fourth-stage juveniles (J3/J4) = 2; males and females = 3; egg-laying females = 4). Statistics were performed on total score. Significant difference is indicated by different letters (P<0.05 in Mann-Whitney U-test).

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    Total number of hatched juveniles from four plants of Oryza sativa, Nicotiana benthamiana and N. tabacum roots.

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