Quantitative trait loci mapping of Meloidogyne incognita and M. hapla resistance in a recombinant inbred line population of soybean

in Nematology
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Summary

A recombinant inbred line population of soybean (Glycine max) was utilised to identify the quantitative trait loci (QTLs) determining the response to infection by two root-knot nematode species, Meloidogyne incognita and M. hapla, in glasshouse assays. QTL analysis detected seven major and four minor QTLs on seven soybean chromosomes ((Chrs) 1, 7, 8, 10, 14, 18, 20) explaining 6-41% phenotypic variance (PVE) for M. incognita root response and nematode reproduction. Three of the major QTLs, on Chrs 7, 10 and 18, were confirmed in previous reports and two major QTLs on Chrs 14 and 20 were detected for the first time. The QTL analysis with M. hapla provides the first report of a major QTL region mapped on Chr 7, explaining 70-82% PVE in M. hapla root response and nematode reproduction. These novel identified QTLs with flanking markers will be helpful in marker-assisted breeding for nematode resistance in soybean.

Nematology

International Journal of Fundamental and Applied Nematological Research

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References

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Figures

  • Distribution of estimated random-effect least squares means of galling index (A), egg masses (g root)−1 (B), and log(eggs(groot)1+1) (C) of Meloidogyne incognita in the recombinant inbred line (RIL) population F2:10 (‘TK780’ × ‘H4’). Data were collected 42 days after inoculation. Galling index: 0-10 scale; 0 = no galling; 10 = severe galling. The arrows point to the phenotypic reaction in ‘TK780’ and ‘H4’.

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  • Distribution of estimated random-effect least squares means of galling index (A), egg masses (g root)−1 (B) and log(eggs(groot)1+1) (C) of Meloidogyne hapla in the recombinant inbred line (RIL) population F2:10 (‘TK780’ × ‘H4’). Data were collected 42 days after inoculation. Galling index: 0-10 scale; 0 = no galling; 10 = severe galling. The arrows point to the phenotypic reaction in ‘TK780’ and ‘H4’.

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  • QTLs associated by nonparametric mapping with root-galling index (GI), egg masses (g root)−1 (EM) and nematode egg production (LogEGR) for both Meloidogyne incognita and M. hapla in recombinant inbred line population ‘TK780’ × ‘H4’.

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  • Map locations of QTLs associated with resistance to Meloidogyne incognita Race 1 and variation in root galling (GI), egg-mass production (EM) and egg production (LogEGR) on soybean. The colour bars showed QTL regions based on single-marker analysis using nonparametric mapping (Kruskal-Wallis analysis) test equivalent of the one-way analysis of variance (Van Ooijen 2004). Black bars = QTL influencing root-galling index phenotype; Grey bars – QTL influencing EM production; White bars = QTL influencing EGR phenotype. The LOD intervals (grey solid line) and the phenotypic variance (% Expt I, black line) of major QTLs associated with GI, EM or LogEGR are presented in graph based on the analysis of pairs of linked markers (Interval Mapping) with genome-wide threshold value of LOD (grey dash line, GI: 3.2; EM: 2.5; LogEGR: 3.0) after 1000 permutation test for all traits at a=0.05.

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  • The phenotypic variance (% Expt I) of the major QTL on Chr 7 associated with Meloidogyne hapla in root galling (GI, black solid line), egg-mass production (EM, black dash line) and egg production (LogEGR, grey line) on soybean based on the analysis of Interval Mapping.

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