A molecular phylogenetic approach for unravelling the taxonomic status of Basilaphelenchus persicus n. gen., n. sp. (Aphelenchoididae: Tylaphelenchinae)

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Summary

Basilaphelenchus persicus n. gen., n. sp. is described and illustrated based on morphological and molecular phylogenetic studies. The new genus belongs to the family Aphelenchoididae and is characterised mainly by the small body size of females (297-393 μm) and males (304-331 μm), high cephalic region in both sexes, fine 5.5-7.8 μm long stylet that is barely visible in mounted specimens yet clear in fresh individuals, stylet with a linear conus and weak shaft ending in three elongate, posteriorly directed, knobs, small spherical metacorpus with posteriorly located (at 72-75% of metacorpus) weak but distinct valve, monodelphic-prodelphic reproductive system with a long post-vulval uterine sac containing large rounded sperm cells, a vulva lacking any flap apparatus, conical tail of female usually ventrally bent at tip and male tail ventrally bent as in the female, lacking the P1 supplement, but with three pairs of caudal papillae, and small arcuate spicules with rounded condylus and small pointed rostrum. The new genus is similar to both Tylaphelenchus and Pseudaphelenchus in the possession of a raised cephalic region and a small spherical metacorpus, placing it in the subfamily Tylaphelenchinae. It is also typologically similar to Aphelenchoides. Molecular phylogenetic studies using both SSU and LSU rRNA gene sequences (both partial) revealed that the new genus formed a robustly supported sister clade with Pseudaphelenchus spp. and had a basal placement to this clade and, probably, to all other aphelenchoidids. Tylaphelenchus grosmannae is transferred to the new genus as B. grosmannae n. comb. and A. christinae n. comb. (= T. christinae) is proposed. The taxonomic status of Tylaphelenchus is discussed and T. sinodendroni n. comb. (= A. sinodendroni) proposed.

Nematology

International Journal of Fundamental and Applied Nematological Research

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Figures

  • Basilaphelenchus persicus n. gen., n. sp. A, B: Entire body of female and male; C: Details of anterior region; D: Pharyngeal region; E: Female reproductive system; F, G: Variation in position of excretory pore; H: Spicules; I: Cross-section of female mid-body; J: Details of male posterior body region and position of papillae (P2-P4, arrowheads).

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  • Basilaphelenchus persicus n. gen., n. sp. Fresh material in temporary slides in water. A: Female posterior body region showing post-vulval uterine sac and sperm inside; B, F: Part of pharynx and details of metacorpus; C: Part of anterior body region; D: Details of anterior end showing high cephalic region, linear stylet conus, and three posteriorly directed knobs, E: Female tail; G: Male posterior body end and spicules (arrowheads showing P3 and P4. P2 is not seen at the same focus as P3 and P4). (Scale bars: A-C, E, G = 10 μm; D, F = 5 μm.)

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  • Basilaphelenchus persicus n. gen., n. sp. Mounted specimens in permanent slides. A, B: Anterior body end; C: Part of pharynx and details of metacorpus and position of excretory pore; D-F: Different parts of female reproductive system (D: Vulva, vagina, uterus, crustaformeria; E, F: Post-vulval uterine sac (PUS) with sperm inside); G, H: Spicules, I: Male posterior body region. (Scale bars = 10 μm.)

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  • Morphometrics of Basilaphelenchus persicus n. gen., n. sp. All measurements are in μm and in the form: mean ± s.d. (range).

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  • (Continued.)

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  • Bayesian 50% majority rule consensus tree inferred from small subunit (SSU) rRNA gene sequences of Basilaphelenchus persicus n. gen., n. sp. under the GTR + G + I model (dataset 1). Bayesian posterior probabilities (BPP) and maximum likelihood bootstrap (ML BS) values >0.50 are given for appropriate clades in the form: BPP/ML BS. The sequence of the new genus is in bold font. The asterisk refers to recent taxonomic revisions proposed by Gu et al. (2017).

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  • Bayesian 50% majority rule consensus tree inferred from small subunit (SSU) rRNA gene sequences of Basilaphelenchus persicus n. gen., n. sp. under the GTR + G + I model (dataset 2). Bayesian posterior probabilities (BPP) and maximum likelihood bootstrap (ML BS) values >0.50 are given for appropriate clades in the form: BPP/ML BS. The sequence of the new genus is in bold font. The asterisk refers to recent taxonomic revisions proposed by Gu et al. (2017).

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  • Bayesian 50% majority rule consensus tree inferred from D2-D3 large subunit (LSU) rRNA gene sequences of Basilaphelenchus persicus n. gen., n. sp. under the GTR + G + I model. Bayesian posterior probabilities (BPP) and maximum likelihood bootstrap (ML BS) values >50% are given for appropriate clades in the form: BPP/ML BS. The new sequence is in bold font.

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  • Bayesian 50% majority rule consensus tree inferred from large subunit (LSU) + small subunit (SSU) rRNA gene sequences of Basilaphelenchus persicus n. gen., n. sp. and the species/isolates sequenced for both 18S and 28S rDNA fragments under the GTR + G + I model. Bayesian posterior probabilities (BPP) and maximum likelihood bootstrap (ML BS) values >0.50 are given for appropriate clades in the form: BPP/ML BS. The new species is in bold font.

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