Bursaphelenchus geraerti n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from the United Arab Emirates

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Summary

Bursaphelenchus geraerti n. sp., isolated in Tianjin, P.R. China, from packaging wood made of Pinus sp. imported from the United Arab Emirates, is described. It is characterised by the lateral field with three lines, a stylet 13-14 μm long with small basal swellings, excretory pore located posterior to nerve ring, spicule mitten-shaped, ventrally arcuate with lamina smoothly tapering towards distal tip, with a distinct bend near 60% of total length, condylus elongated with rounded tip, rostrum conical with finely rounded tip, and cucullus weakly developed or absent. The bursal flap is wide and spade-like with the posterior margin truncate or rounded and starts posterior to P6 gland papillae. A vulval flap is absent and the tail is conical, strongly ventrally curved with the terminus pointed and sometimes appearing as a mucron. The new species is characterised by morphological and molecular analysis and is close to B. decraemerae, B. gerberae and B. sakishimanus.

Bursaphelenchus geraerti n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from the United Arab Emirates

in Nematology

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References

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Figures

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    Bursaphelenchus geraerti n. sp. A: Female; B: Male; C: Anterior body region showing lateral field; D: Head region; E: Male tail in ventral view showing caudal papillae and bursa; F: Male tail in lateral view; G: Male spicules; H: Female gonad; I: Female tails. (Scale bars = 10 μm.)

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    Light micrographs of Bursaphelenchus geraerti n. sp. male. A: Entire body; B, C: Tail in ventral view showing caudal papillae and bursa; D-G: Tail in lateral ventral view (caudal papillae = P + number). (Scale bars = 10 μm.)

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    Light micrographs of Bursaphelenchus geraerti n. sp. female. A: Entire body; B, C: Pharyngeal region showing excretory pore (arrow); D: Vulval region in lateral view (vulva visible in posterior part); E: Post-uterine sac region; F: Lateral field; G-I: Tail. (Scale bars = 10 μm.)

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    Scanning electron microscopy of Bursaphelenchus geraerti n. sp. Male tail showing papillal arrangement (P + number). (Scale bar = 10 μm.)

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    Morphometrics of Bursaphelenchus geraerti n. sp. All measurements are in μm and in the form: mean ± s.d. (range).

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    ITS-RFLP patterns of Bursaphelenchus geraerti n. sp. Restriction fragments were obtained by digestion of the amplified rDNA fragment (1) with RsaI (2), HaeIII (3), MspI (4), HinfI (5) and AluI (6). M = DNA marker (100 bp ladder, Invitrogen Life Technologies).

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    Sizes of DNA restriction fragments obtained for Bursaphelenchus geraerti n. sp. and B. decraemerae in ITS-RFLP analysis and calculated on sequencing results.

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    Phylogenetic relationships of Bursaphelenchus geraerti n. sp. and aphelenchid nematodes based on concatenated dataset (partial 18S rDNA + full length of ITS rDNA + partial 28S D2-D3 rDNA). The 10001st Bayesian tree was inferred under GTR + I + G model, Aphelenchoides besseyi served as the outgroup species. Posterior probability values exceeding 50% are given on appropriate clades.

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    Sequences of nematode species used for the phylogenetic analyses.

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    (Continued.)

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