Is Phasmarhabditis papillosa (Nematoda: Rhabditidae) a possible biological control agent against the Spanish slug, Arion vulgaris (Gastropoda: Arionidae)?
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Summary
In the period from August to October 2018, 140 specimens of the Spanish slug, Arion vulgaris, were collected from Podbrezje, Slovenia. Slugs were dissected and examined for the presence of parasitic nematodes within the cadavers. Identification of the nematodes was conducted using morphological and molecular techniques and confirmed the presence of Phasmarhabditis papillosa. This is the first record of P. papillosa from the mollusc host, A. vulgaris. Laboratory experiments aimed at testing the efficacy of P. papillosa against A. vulgaris were conducted using nematodes grown in vivo. Nematodes were applied at concentration rates of 50, 100 and 200 nematodes slug−1, respectively. Three weeks following treatment, the mortality of slugs was confirmed in all treatments (50 nematodes slug−1, 37.4 ± 2.7%; 100 nematodes slug−1, 48.4 ± 2.7%; 200 nematodes slug−1, 50.6 ± 2.7%). However, the pathogenesis of P. papillosa was observed first in the treatments with the lowest nematode dose at 4 days after treatments, while a decrease in the feeding behaviour of slugs was noted first in the treatments with the highest nematode dose. Future opportunities for the potential use of P. papillosa as a biological control agent against slugs are discussed. This is the first report of P. papillosa from Slovenia, and of its virulence against A. vulgaris.
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Is Phasmarhabditis papillosa (Nematoda: Rhabditidae) a possible biological control agent against the Spanish slug, Arion vulgaris (Gastropoda: Arionidae)?
In: NematologySearch for other papers by Žiga Laznik in
Current site
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PubMed
Search for other papers by Ivana Majić in
Current site
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PubMed
Search for other papers by Stanislav Trdan in
Current site
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PubMed
Search for other papers by Antoinette P. Malan in
Current site
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PubMed
Search for other papers by Annika Pieterse in
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PubMed
University of Aberdeen, School of Biological Sciences, Aberdeen AB24 3UU, UK
Search for other papers by Jenna L. Ross in
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PubMed
Summary
In the period from August to October 2018, 140 specimens of the Spanish slug, Arion vulgaris, were collected from Podbrezje, Slovenia. Slugs were dissected and examined for the presence of parasitic nematodes within the cadavers. Identification of the nematodes was conducted using morphological and molecular techniques and confirmed the presence of Phasmarhabditis papillosa. This is the first record of P. papillosa from the mollusc host, A. vulgaris. Laboratory experiments aimed at testing the efficacy of P. papillosa against A. vulgaris were conducted using nematodes grown in vivo. Nematodes were applied at concentration rates of 50, 100 and 200 nematodes slug−1, respectively. Three weeks following treatment, the mortality of slugs was confirmed in all treatments (50 nematodes slug−1, 37.4 ± 2.7%; 100 nematodes slug−1, 48.4 ± 2.7%; 200 nematodes slug−1, 50.6 ± 2.7%). However, the pathogenesis of P. papillosa was observed first in the treatments with the lowest nematode dose at 4 days after treatments, while a decrease in the feeding behaviour of slugs was noted first in the treatments with the highest nematode dose. Future opportunities for the potential use of P. papillosa as a biological control agent against slugs are discussed. This is the first report of P. papillosa from Slovenia, and of its virulence against A. vulgaris.
| All Time | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 625 | 172 | 13 |
| Full Text Views | 46 | 9 | 0 |
| PDF Views & Downloads | 71 | 8 | 0 |