An undescribed Cryptaphelenchus species was isolated from a bark beetle, Cryphalus fulvus, collected from the inner bark of a dead Japanese red pine, Pinus densiflora, in Kyoto, Japan. The nematode is described as a new species, Cryptaphelenchus fulvus n. sp., characterised by the presence of seven genital papillae in the male arranged as 1 + 2 + 2 + 2; a bursal flap-like cuticular extension surrounding the male tail, and intraspecific variation in the female tail, which ranges from broad to conical in overall shape, with the tip pointed, digitate, or rounded. Additionally, females with a broad tail possess a small papilla-like projection on the dorsal side of the tail near the tip, a unique feature of this species. Phylogenetically, the new species is closely related to Cryptaphelenchus orientalis, C. varicaudatus, and C. paravaricaudatus, but can be differentiated from these species based on molecular phylogenetics, several typological characters and morphometric values.
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Aliramaji, F., Miraeiz, E., Pourjam, E. & Heydari, R. (2019). Molecular and morphological characterisation of Cryptaphelenchus baujardi n. sp. (Nematoda: Ektaphelenchinae), a new species based on two populations from Iran. Nematology 21, 45-56. DOI: 10.1163/15685411-00003194
Atighi, M.R., Pourjam, E., Pedram, M., Ye, W. & Aliramaji, F. (2013). Molecular and morphological characterization of Ektaphelenchoides andrassyi sp. n. (Rhabditida, Aphelenchoididae) from northern Iran. Journal of Nematode Morphology and Systematics 16, 17-23.
Carletti, B. (2008). Bursaphelenchus species with their natural vectors in Italy: distribution and essential diagnostic features. Redia 91, 111-117.
Dod, N., Pernek, M. & Carletti, B. (2013). Pinewood nematodes – as a factor of pine decline in Croatia. In: Schröder, T. (Ed.). Pine wilt disease. Conference 2013, 15-18 October 2013, Braunschweig, Germany, Julius Kühn-Institut, pp. 131-132. DOI: 10.5073/berjki.2013.169.000
Fonseca, L., Cardoso, J.M.S., Lopes, A., Pestana, M., Abreu, F., Nunes, N., Mota, M. & Abrantes, I. (2012). The pinewood nematode, Bursaphelenchus xylophilus, in Madeira Island. Helminthologia 49, 96-103. DOI: 10.2478/s11687-012-0020-3
Fuchs, A.G. (1930). Neue an Borken- und Rüsselkäfer gebundene Nematoden, halbparasitische und Wohnungseinmieter. Zoologische Jahrbücher, Abteilung für Systematik, Ökologie und Geographie der Tiere 59, 505-646.
Fuchs, A.G. (1937). Neue parasitische und halbparasitische Nematoden bei Borkenkäfern und einige andere Nematoden. I. Teil. Zoologische Jahrbücher, Abteilung für Systematik, Ökologie und Geographie der Tiere 70, 291-380.
Gu, J., Braasch, H., Burgermeister, W., Brandstetter, M. & Zhang, J. (2006). Description of Bursaphelenchus yongensis sp. n. (Nematoda: Parasitaphelenchidae) isolated from Pinus massoniana in China. Russian Journal of Nematology 14, 91-99.
Gu, J., Fang, E. & Ma, X. (2022). Description of Cryptaphelenchus recticaudatus n. sp. (Aphelenchoidea: Ektaphelenchinae) in Pinus elliottii from the USA. Nematology 24, 465-473. DOI: 10.1163/15685411-bja10142
Gu, J., Ma, X., Castillo, P. & Munawar, M. (2023). Description of two new Cryptaphelenchus species from China and Japan. Nematology 25, 479-493. DOI: 10.1163/15685411-bja10234
Gu, J., Castillo, P., Ma, X. & Munawar, M. (2024). Unveiling novel and known Cryptaphelenchus species from China and USA. Nematology 26, 731-752. DOI: 10.1163/15685411-bja10335
Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hordijk, W. & Gascuel, O. (2010). New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology 59, 307-321. DOI: 10.1093/sysbio/syq010
Hayashi, M., Kimoto, S. & Morimoto, K. (Eds) (1984). The Coleoptera of Japan in color, Vol. 4. Tokyo, Japan, Hoikusha.
Hooper, D.J. (1986). Handling, fixing, staining and mounting nematodes. In: Southey, J.F. (Ed.). Laboratory methods for work with plant and soil nematodes, 6th edition. London, UK, Her Majesty’s Stationery Office, pp. 59-80.
Huelsenbeck, J.P. & Ronquist, F. (2001). MR BAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17, 1754-1755. DOI: 10.1093/bioinformatics/17.8.754
Hunt, D.J. (1993). Aphelenchida, Longidoridae and Trichodoridae: their systematics and bionomics. Wallingford, UK, CAB International.
Hunt, D.J. (2008). A checklist of the Aphelenchoidea (Nematoda: Tylenchina). Journal of Nematode Morphology and Systematics 10(2007), 99-135.
Kakulia, G.A. (1967). [New nematode genus, Devibursaphelenchus n. g. (Nematoda: Aphelenchoididae).] Soobschcheniya Akademii Nauk Gruzinskoi SSR 47, 439-443.
Kanzaki, N. (2013). Simple methods for morphological observation of nematodes. Nematological Research (Japanese Journal of Nematology) 43, 15-17. DOI: 10.3725/jjn.43.15
Kanzaki, N. & Futai, K. (2002). A PCR primer set for determination of phylogenetic relationships of Bursaphelenchus species within the xylophilus group. Nematology 4, 35-41. DOI: 10.1163/156854102760082186
Kanzaki, N. & Fjellberg, A. (2025). Re-isolation of Ektaphelenchus winteri (Hooper, 1995) Heydari & Pedram, 2020 (Rhabditida: Aphelenchoididae) from Xylodiplosis nigritarsis (Diptera: Cecidomyiidae) in Norway. Nematology 27, 221-236. DOI: 10.1163/15685411-bja10384
Kanzaki, N. & Giblin-Davis, R.M. (2012). Aphelenchoidea. In: Manzanilla-López, R.H. & Marbán-Mendoza, N. (Eds). Practical plant nematology. Jalisco, Mexico, Colegio de Post-graduados and Mundi-Prensa, Biblioteca Básica de Agricultura, pp. 161-208.
Kanzaki, N., Ekino, T., Hamaguchi, K. & Takeuchi-Kaneko, Y. (2021). Three Seinura species from Japan with a description of S. shigaensis n. sp. (Tylenchomorpha: Aphelenchoididae). PLoS ONE 16, e0244653. DOI: 10.1371/journal.pone.0244653
Kanzaki, N., Ekino, T. & Degawa, Y. (2022). Cryptaphelenchus abietis n. sp. (Tylenchomorpha: Aphelenchoididae) isolated from Cryphalus piceae (Ratzeburg) (Coleoptera: Scolytinae) emerged from Abies veitchii Lindl. (Pinaceae) from Nagano, Japan. Nematology 24, 65-84. DOI: 10.1163/15685411-bja10112
Katoh, K., Misawa, K., Kuma, K. & Miyata, T. (2002). MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research 30, 3059-3066. DOI: 10.1093/nar/gkf436
Kikuchi, T., Aikawa, T., Oeda, Y., Karim, N. & Kanzaki, N. (2009). A rapid and precise diagnostic method for detecting the pinewood nematode Bursaphelenchus xylophilus by loop-mediated isothermal amplification (LAMP). Phytopathology 99, 1365-1369. DOI: 10.1094/PHYTO-99-12-1365
Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018). MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution 35, 1547-1549. DOI: 10.1093/molbev/msy096
Kuraku, S., Zmasek, C.M., Nishimura, O. & Katoh, K. (2013). aLeaves facilitates on-demand exploration of metazoan gene family trees on MAFFT sequence alignment server with enhanced interactivity. Nucleic Acids Research 41, W22-W28. DOI: 10.1093/nar/gkt389
Larget, B. & Simon, D.L. (1999). Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution 16, 750-759. DOI: 10.1093/oxfordjournals.molbev.a026160
Lefort, V., Longueville, J.-E. & Gascuel, O. (2017). SMS: smart model selection in PhyML. Molecular Biology and Evolution 34, 2422-2424. DOI: 10.1093/molbev/msx149
Minagawa, N. & Mizukubo, T. (1994). A simplified procedure of transferring nematodes to glycerol for permanent mounts. Nematological Research (Japanese Journal of Nematology) 24, 75. DOI: 10.3725/jjn1993.24.2_75
Pedram, M. (2017). Cryptaphelenchus varicaudatus n. sp. (Rhabditida: Ektaphelenchinae) from Tehran Province, Iran. Journal of Nematology 49, 223-230. DOI: 10.21307/jofnem-2017-066
Pedram, M., Gu, J., Liu, L. & Guo, K. (2020). Description of Cryptaphelenchus paravaricaudatus n. sp. (Aphelenchoidea: Ektaphelenchinae) from Pinus massoniana Lamb. in China. Nematology 22, 723-732. DOI: 10.1163/15685411-00003335
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012). MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61, 539-542. DOI: 10.1093/sysbio/sys029
Rühm, W. (1956). Die Nematoden der Ipiden. Parasitologische SchriftenReihe 6, 1-435.
Rühm, W. & Chararas, C. (1957). Description, biologie et histologie de quatre espèces nouvelles de nématodes parasites de Dryocoetes hectographus Reit. (Col. Scolytidae). Entomophaga 2, 253-269. DOI: 10.1007/BF02373597
Ryss, A., Vieira, P., Mota, M.M. & Kulinich, O. (2005). A synopsis of the genus Bursaphelenchus Fuchs, 1937 (Aphelenchida: Parasitaphelenchidae) with keys to species. Nematology 7, 393-458. DOI: 10.1163/156854105774355581
Ryss, A.Y. & Subbotin, S.A. (2017). [Coevolution of wood-inhabiting nematodes of the genus Bursaphelenchus Fuchs, 1937 with their insect vectors and plant hosts.] Zhurnal Obshchei Biologii 78, 32-61.
Tanaka, R., Kikuchi, T., Aikawa, T. & Kanzaki, N. (2012). Simple and quick methods for nematode DNA preparation. Applied Entomology and Zoology 47, 291-294. DOI: 10.1007/s13355-012-0115-9
Torrini, G., Strangi, A., Paoli, F., Binazzi, F., Camerota, M., Pennacchio, F. & Roversi, P.F. (2017). A new phoretic association: Bursaphelenchus minutus (Nematoda: Parasitaphelenchidae) and Orthotomicus erosus (Coleoptera: Scolytidae) recorded on Pinus pinea (L.). Scandinavian Journal of Forest Research 32, 455-458. DOI: 10.1080/02827581.2016.1254278
Walia, K.K., Negi, G., Bajaj, H.K. & Kalia, D.C. (2003). Two new species of Bursaphelenchus Fuchs, 1937 (Nematoda: Aphelenchoididae) from pine wood and insect frass from India. Indian Journal of Nematology 33, 1-5.
Ye, W., Giblin-Davis, R.M., Braasch, H., Morris, K. & Thomas, W.K. (2007). Phylogenetic relationships among Bursaphelenchus species (Nematoda: Parasitaphelenchidae) inferred from nuclear ribosomal and mitochondrial DNA sequence data. Molecular Phylogenetics and Evolution 43, 1185-1197. DOI: 10.1016/j.ympev.2007.02.006
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An undescribed Cryptaphelenchus species was isolated from a bark beetle, Cryphalus fulvus, collected from the inner bark of a dead Japanese red pine, Pinus densiflora, in Kyoto, Japan. The nematode is described as a new species, Cryptaphelenchus fulvus n. sp., characterised by the presence of seven genital papillae in the male arranged as 1 + 2 + 2 + 2; a bursal flap-like cuticular extension surrounding the male tail, and intraspecific variation in the female tail, which ranges from broad to conical in overall shape, with the tip pointed, digitate, or rounded. Additionally, females with a broad tail possess a small papilla-like projection on the dorsal side of the tail near the tip, a unique feature of this species. Phylogenetically, the new species is closely related to Cryptaphelenchus orientalis, C. varicaudatus, and C. paravaricaudatus, but can be differentiated from these species based on molecular phylogenetics, several typological characters and morphometric values.
All Time | Past 365 days | Past 30 Days | |
---|---|---|---|
Abstract Views | 77 | 77 | 32 |
Full Text Views | 4 | 4 | 2 |
PDF Views & Downloads | 19 | 19 | 11 |