Randomly selected ecosystems in the coastal Greater Vancouver, Fraser valley and southern Vancouver Island regions, and in the interior Okanagan region of British Columbia, were sampled for the presence of entomopathogenic nematodes (EPN). Of 133 soil samples, 23 contained EPN (18 with steinernematids and five with heterorhabditids). Three identified species and one unidentified species were found. Steinernema affine and S. kraussei occurred in most EPN positive locations in the coastal regions sampled. Steinernema affine was found in open habitats and S. kraussei in all types of tree habitats. Steinernema feltiae was found in only one location, White Rock, in the Greater Vancouver region, close to an ocean beach. Heterorhabditis megidis occurred in only the drier Okanagan valley region of central, southern British Columbia, which has a more extreme climate than the moist, moderate climate of the coastal region. It occurred mostly in agricultural land represented by orchards, vineyards, and secondary woodland. In field baiting and laboratory experiments S. affine appeared to be a more successful parasite than S. kraussei.