A survey of entomopathogenic nematodes from 38 golf courses was conducted in different climatic areas of Ontario and Québec provinces, Canada, during autumn 2002 and 2003. Soil samples were collected on golf course greens, fairways and roughs. Entomopathogenic nematodes were isolated from soil using wax moth (Galleria mellonella) and carrot weevil (Listronotus oregonensis) larvae as bait. Of the 436 samples (218 sites), 11 contained entomopathogenic nematodes for this study. All entomopathogenic nematode-positive samples were isolated from roughs. Isolates were characterised using DNA satellite probes and 28S rDNA sequence data. Both methods confirmed the identities of nematode isolates as Steinernema carpocapsae, S. feltiae and S. kraussei.