Recognizing the importance of increasing the levels of children's humane behavior toward animals other than humans relates to the developing of valid and reliable measures of such behavior. This study reports the psychometric properties of the Children's Treatment of Animals Questionnaire (CTAQ), which assesses children's humane behavior toward nonhuman animals. The findings, based on self-reports by 61 elementary school children (25 boys; 36 girls), showed that the 13-item scale has adequate internal consistency. In addition, comparing two administrations of the scale over a five-week period demonstrated good test-retest reliability. The scale's convergent validity was demonstrated with significant correlations between responses on the CTAQ and two previously validated measures of empathy. The study concluded that the CTAQ is a valid and reliable measure for assessing the degree to which children's behavior toward nonhuman animals is humane. Determining the sensitivity of the measure to change (following humane education) and the predictive validity of the measure (identification of children who are cruel to animals) will require further research.