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Brazilian bentonite and a new modified bentonite material, BAC302, reduce zearalenone-induced cell death

In: World Mycotoxin Journal
Authors:
J. Nones Department of Food and Chemical Engineering, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
Vale do Itajaí University, Balneário Camboriú, Santa Catarina State, Brazil.

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A. Solhaug Norwegian Veterinary Institute, Ullevaalsveien 68, Oslo 0454, Norway.

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H.G. Riella Department of Food and Chemical Engineering, Federal University of Santa Catarina, Florianópolis, SC, Brazil.

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G.S. Eriksen Norwegian Veterinary Institute, Ullevaalsveien 68, Oslo 0454, Norway.

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J. Nones Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
Integrated Company for Agricultural Development of Santa Catarina, Florianópolis, SC, Brazil.

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Bentonite clays exhibit high adsorptive capacity for contaminants and is frequently used as a feed additive to reduce the bioavailability and thus the toxicity of several mycotoxins. Zearalenone (ZEN) is a secondaryFusarium toxic metabolite that can contaminate a wide range of food- and feedstuff. Since organophilic treatments is known to increase the adsorption capacity of bentonites, the aim of study was to evaluate and compare the ability of natural bentonite and bentonite treated with BAC302 to protect against ZEN-induced cytotoxicity in the epithelial colorectal adenocarcinoma (Caco-2) and human leukemia monocytic (THP-1) cell lines. The two materials were not toxic to the cell lines at lower concentrations. Furthermore, the results indicate that the two materials protect the Caco-2 and THP-1 cells against ZEN-induced cytotoxicity, probably by extracellular adsorption of ZEN. The tested natural bentonite shows potential forin vivo testing to evaluate if it is suitable for intoxication in ZEN contaminated animal feeds.

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