Ethiopia is sub-Saharan Africa’s greatest wheat producer. However, its production, productivity and utilisations are constrained by fungal infections, which lead to yield losses and mycotoxin contaminations. The present study was aimed to determined fungal species and multi-mycotoxins in wheat grain samples (n=178) collected during the 2020 cropping season in Ethiopia. Different fungal genera ofAlternaria,Aspergillus,Fusarium,Penicillium, andRhizopus species were isolated from the infected grain samples, of whichFusarium species were abundantly prevalent. Multi-mycotoxin analysis done using liquid chromatography-tandem mass spectrometric (LC-MS/MS). Overall, 49 metabolites of different fungal species including masked, regulated and emerging mycotoxins were detected and categorised into eight groups. Among major mycotoxins and derivatives, deoxynivalenol was frequently detected in about 70.8% of samples with the maximum concertation 15,900 μg/kg, followed by its masked metabolites of deoxynivalenol-3-glucoside detected in 64% of the samples with maximum level 2,120 μg/kg. AmongFusarium metabolites, 15-hydroxyculmorin was detected in 46.6% of wheat samples with maximum level of 4,290 μg/kg, while chrysogin, enniatin B and culmorin were detected in 58.4, 43.3 and 44.9%, of the wheat samples with maximum levels of 154, 325 and 2,860 μg/kg, respectively. AmongAlternaria metabolites tenuazonic acid was most prevalent mycotoxin occurring in 79.2% of samples with maximum level (236 μg/kg). Furthermore, metabolites from other fungal genera were detected at different levels. The widespread presence and high amount of multi-mycotoxin in wheat samples indicated proportionately higher exposure levels, which could result in serious food safety and health consequences. Integrating several management strategies both before and after harvest may lower the risk of mycotoxin contamination in wheat, being used as diversified food products in the country.
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Alkadri, D., Nipoti, P., Döll, K., Karlovsky, P., Prodi, A. and Pisi, A., 2013. Study of fungal colonization of wheat kernels in Syria with a focus onFusarium species. International Journal of Molecular Sciences 14: 5938-5951.
'Study of fungal colonization of wheat kernels in Syria with a focus onFusarium species ' () 14 International Journal of Molecular Sciences : 5938 -5951.
Alkadri, D., Rubert, J., Prodi, A., Pisi, A., Mañes, J. and Soler, C., 2014. Natural co-occurrence of mycotoxins in wheat grains from Italy and Syria. Food Chemistry 157: 111-118.
'Natural co-occurrence of mycotoxins in wheat grains from Italy and Syria ' () 157 Food Chemistry : 111 -118.
Ayalew, A., 2010. Mycotoxins and surface and internal fungi of maize from Ethiopia. African Journal of Food, Agriculture, Nutrition and Development 10: 4109-4123.
'Mycotoxins and surface and internal fungi of maize from Ethiopia ' () 10 African Journal of Food, Agriculture, Nutrition and Development : 4109 -4123.
Ayalew, A., Fehmann, H., Lepschy, J., Beck, R. and Abate, D., 2006. Natural occurrence of mycotoxins in staple cereals from Ethiopia. Mycopathologia 162: 57-63.
'Natural occurrence of mycotoxins in staple cereals from Ethiopia ' () 162 Mycopathologia : 57 -63.
Bai, G., and Shaner, G., 2004. Management and resistance in wheat and barley toFusarium head blight. Annual Review of Phytopathology 42: 135-161.
'Management and resistance in wheat and barley toFusarium head blight ' () 42 Annual Review of Phytopathology : 135 -161.
Buczacki, S.T., Attenborough, D. and Wilkinson, J., 1989. New generation guide to the fungi of Britain and Europe. University of Texas Press, Austin, TX, USA.
'New generation guide to the fungi of Britain and Europe', ().
Chala, A., Taye, W., Ayalew, A., Krska, R., Sulyok, M. and Logrieco, A., 2014. Multimycotoxin analysis of sorghum (Sorghum bicolor L. Moench) and finger millet (Eleusine coracana L. Garten) from Ethiopia. Food Control 45: 29-35.
'Multimycotoxin analysis of sorghum (Sorghum bicolor L. Moench) and finger millet (Eleusine coracana L. Garten) from Ethiopia ' () 45 Food Control : 29 -35.
Cheli, F., Pinotti, L., Novacco, M., Ottoboni, M., Tretola, M. and Dell’Orto, V., 2017. Mycotoxins in wheat and mitigation measures. In: Wanyera, R. and Owuoche, J. (eds.) Wheat improvement, management and utilization. IntechOpen, London, UK, pp. 227-251.
'Mycotoxins in wheat and mitigation measures ', () 227 -251.
Cheli, F., Pinotti, L., Rossi, L. and Dell’Orto, V., 2013. Effect of milling procedures on mycotoxin distribution in wheat fractions: a review. LWT – Food Science and Technology 54: 307-314.
'Effect of milling procedures on mycotoxin distribution in wheat fractions: a review ' () 54 LWT – Food Science and Technology : 307 -314.
Chilaka, C.A., De Boevre, M., Atanda, O.O. and De Saeger, S., 2017. The status ofFusarium mycotoxins in sub-Saharan Africa: a review of emerging trends and post-harvest mitigation strategies towards food control. Toxins 9: 19.
'The status ofFusarium mycotoxins in sub-Saharan Africa: a review of emerging trends and post-harvest mitigation strategies towards food control ' () 9 Toxins : 19.
Central Statistical Agency (CSA), 2021. The Federal Democratic Republic of Ethiopia Central Statistical Agency. Agricultural sample survey in 2020/2021. Volume I. Report on area and production of major crops (Private Peasant Holdings, Meher Season). Statistical Bulletin 586. Adis Ababa, Ethiopia.
Del Ponte, E.M., Garda-Buffon, J. and Badiale-Furlong, E., 2012. Deoxynivalenol and nivalenol in commercial wheat grain related toFusarium head blight epidemics in southern Brazil. Food Chemistry 132: 1087-1091.
'Deoxynivalenol and nivalenol in commercial wheat grain related toFusarium head blight epidemics in southern Brazil ' () 132 Food Chemistry : 1087 -1091.
Dong, F., Wang, S., Yu, M., Sun, Y., Xu, J. and Shi, J. 2017. Natural occurrence of deoxynivalenol and deoxynivalenol-3-glucoside in various wheat cultivars grown in Jiangsu province, China. World Mycotoxin Journal 10: 285-293.
'Natural occurrence of deoxynivalenol and deoxynivalenol-3-glucoside in various wheat cultivars grown in Jiangsu province, China ' () 10 World Mycotoxin Journal : 285 -293.
European Food Safety Authority (EFSA), 2014. Scientific opinion on the risks to human and animal health related to the presence of beauvericin and enniatins in food and feed. EFSA Journal 12: 3802.
Ekwomadu, T.I., Akinola, S.A. and Mwanza, M., 2021.Fusarium mycotoxins, their metabolites (free, emerging, and masked), food safety concerns, and health impacts. International Journal of Environmental Research and Public Health 18: 11741.
'Fusarium mycotoxins, their metabolites (free, emerging, and masked), food safety concerns, and health impacts ' () 18 International Journal of Environmental Research and Public Health : 11741.
Escrivá, L., Oueslati, S., Font, G. and Manyes, L., 2017.Alternaria mycotoxins in food and feed: an overview. Journal of Food Quality 2017: 1569748.
'Alternaria mycotoxins in food and feed: an overview ' () 2017 Journal of Food Quality : 1569748.
European Commission (EC), 2007. Commission Regulation 1126/2007 of 28 September 2007 setting maximum levels for certain contaminants in foodstuffs as regardsFusarium toxins in maize and maize products. Official Journal of the European Union L 255: 14-17.
Ezekiel, C.N., Bandyopadhyay, R., Sulyok, M., Warth, B. and Krska, R., 2012. Fungal and bacterial metabolites in commercial poultry feed from Nigeria. Food Additives and Contaminants Part A 29: 1288-1299.
'Fungal and bacterial metabolites in commercial poultry feed from Nigeria ' () 29 Food Additives and Contaminants Part A : 1288 -1299.
FAOSTAT, 2020. Crops and livestock products. Available at:https://www.fao.org/faostat/en/#data/QCL.
Gab-Allah, M.A., Tahoun, I.F., Yamani, R.N., Rend, E.A. and Shehata, A.B., 2022. Natural occurrence of deoxynivalenol, nivalenol and deoxynivalenol-3-glucoside in cereal-derived products from Egypt. Food Control 137: 108974.
'Natural occurrence of deoxynivalenol, nivalenol and deoxynivalenol-3-glucoside in cereal-derived products from Egypt ' () 137 Food Control : 108974.
Geremew, T., Haesaert, G., Abate, D. and Audenaert, K., 2018. An HPLC-FLD method to measure ochratoxin A in teff (Eragrostis tef) and wheat (Triticum spp.) destined for the local Ethiopian market. World Mycotoxin Journal 11: 359-368.
'An HPLC-FLD method to measure ochratoxin A in teff (Eragrostis tef) and wheat (Triticum spp.) destined for the local Ethiopian market ' () 11 World Mycotoxin Journal : 359 -368.
Getachew, A., Chala, A., Hofgaard, I.S., Brurberg, M.B., Sulyok, M. and Tronsmo, A.M., 2018. Multimycotoxin and fungal analysis of maize grains from south and southwestern Ethiopia. Food Additives and Contaminants Part B 11: 64-74.
'Multimycotoxin and fungal analysis of maize grains from south and southwestern Ethiopia ' () 11 Food Additives and Contaminants Part B : 64 -74.
Getahun, M., Fininsa, C., Bekeko, Z. and Mohammed, A., 2022. Analysis of the spatial distribution and association of wheatFusarium head blight with biophysical factors in Ethiopia. European Journal of Plant Pathology 164: 391-410.
'Analysis of the spatial distribution and association of wheatFusarium head blight with biophysical factors in Ethiopia ' () 164 European Journal of Plant Pathology : 391 -410.
Ghiasian, S.A., Kord-Bacheh, P., Rezayat, S.M., Maghsood, A.H. and Taherkhani, H., 2004. Mycoflora of Iranian maize harvested in the main production areas in 2000. Mycopathologia 158: 113-121.
'Mycoflora of Iranian maize harvested in the main production areas in 2000 ' () 158 Mycopathologia : 113 -121.
Grote, U., Fasse, A., Nguyen, T.T., and Erenstein, O., 2021. Food security and the dynamics of wheat and maize value chains in Africa and Asia. Frontiers in Sustainable Food Systems 4: 617009
'Food security and the dynamics of wheat and maize value chains in Africa and Asia ' () 4 Frontiers in Sustainable Food Systems : 617009.
Gruber-Dorninger, C., Novak, B., Nagl, V. and Berthiller, F., 2017. Emerging mycotoxins: beyond traditionally determined food contaminants. Journal of Agricultural and Food Chemistry 65: 7052-7070.
'Emerging mycotoxins: beyond traditionally determined food contaminants ' () 65 Journal of Agricultural and Food Chemistry : 7052 -7070.
Hailu, E. and Woldeab, G., 2015. Survey of rust andSeptoria leaf blotch diseases of wheat in central Ethiopia and virulence diversity of stem rustPuccinia graminis f. sp.tritici. Advances in Crop Science and Technology 3: 166.
'Survey of rust andSeptoria leaf blotch diseases of wheat in central Ethiopia and virulence diversity of stem rustPuccinia graminis f. sp.tritici ' () 3 Advances in Crop Science and Technology : 166.
Hathout, A.S., Abel-Fattah, S.M., Abou-Sree, Y.H. and Fouzy, A.S., 2020. Incidence and exposure assessment of aflatoxins and ochratoxin A in Egyptian wheat. Toxicology Reports 7: 867-873.
'Incidence and exposure assessment of aflatoxins and ochratoxin A in Egyptian wheat ' () 7 Toxicology Reports : 867 -873.
Jestoi, M., Ritieni, A. and Rizzo, A., 2004. Analysis of theFusarium mycotoxinsfusaproliferin andtrichothecenes in grains using gas chromatography-mass spectrometry. Journal of Agricultural and Food Chemistry 52: 1464-1469.
'Analysis of theFusarium mycotoxinsfusaproliferin andtrichothecenes in grains using gas chromatography-mass spectrometry ' () 52 Journal of Agricultural and Food Chemistry : 1464 -1469.
Kifer, D., Sulyok, M., Jakšić, D., Krska, R. and Šegvić Klarić, M., 2021. Fungi and their metabolites in grain from individual households in Croatia. Food Additives and Contaminants Part B 14: 98-109.
'Fungi and their metabolites in grain from individual households in Croatia ' () 14 Food Additives and Contaminants Part B : 98 -109.
Kruskal, W.H. and Wallis, W.A., 1952. Use of ranks in one-criterion variance analysis. Journal of the American Statistical Association 47: 583-621.
'Use of ranks in one-criterion variance analysis ' () 47 Journal of the American Statistical Association : 583 -621.
Kumar, A., Mishra, V.K., Vyas, R.P. and Singh, V., 2011. Heterosis and combining ability analysis in bread wheat (Triticum aestivum L.). Journal of Plant Breeding and Crop Science 3: 209-217.
'Heterosis and combining ability analysis in bread wheat (Triticum aestivum L.) ' () 3 Journal of Plant Breeding and Crop Science : 209 -217.
Leslie, J.F. and Summerell, B. A., 2006. TheFusarium laboratory manual. Blackwell Publishing, Hoboken, NJ, USA.
'TheFusarium laboratory manual', ().
Lević, J., Stanković, S., Krnjaja, V., Tančić, S., Ivanović, D. and Bočarov-Stančić, A., 2012. Relationships of mycobiota on rachides and kernels of wheat. European Journal of Plant Pathology 134: 249-256.
'Relationships of mycobiota on rachides and kernels of wheat ' () 134 European Journal of Plant Pathology : 249 -256.
Li, F., Jiang, D., Zhou, J., Chen, J., Li, W. and Zheng, F., 2016. Mycotoxins in wheat flour and intake assessment in Shandong province of China. Food Additives and Contaminants Part B 9: 170-175.
'Mycotoxins in wheat flour and intake assessment in Shandong province of China ' () 9 Food Additives and Contaminants Part B : 170 -175.
Liu, Y. and Wu, F., 2010. Global burden of aflatoxin-induced hepatocellular carcinoma: a risk assessment. Environmental Health Perspectives 118: 818-824.
'Global burden of aflatoxin-induced hepatocellular carcinoma: a risk assessment ' () 118 Environmental Health Perspectives : 818 -824.
Logrieco, A., Rizzo, A., Ferracane, R. and Ritieni, A., 2002. Occurrence of beauvericin and enniatins in wheat affected byFusarium avenaceum head blight. Applied and Environmental Microbiology 68: 82-85.
'Occurrence of beauvericin and enniatins in wheat affected byFusarium avenaceum head blight ' () 68 Applied and Environmental Microbiology : 82 -85.
Mamo, F.T., Abate, B.A., Tesfaye, K., Nie, C., Wang, G. and Liu, Y., 2020. Mycotoxins in Ethiopia: a review on prevalence, economic and health impacts. Toxins 12: 648.
'Mycotoxins in Ethiopia: a review on prevalence, economic and health impacts ' () 12 Toxins : 648.
Mashinini, K. and Dutton, M.F., 2006. The incidence of fungi and mycotoxins in South Africa wheat and wheat-based products. Journal of Environmental Science and Health Part B 41: 285-296.
'The incidence of fungi and mycotoxins in South Africa wheat and wheat-based products ' () 41 Journal of Environmental Science and Health Part B : 285 -296.
Mohammed, A., Bekeko, Z., Yusufe, M., Sulyok, M. and Krska, R., 2022. Fungal species and multi-mycotoxin associated with postharvest sorghum (Sorghum bicolor (L.) Moench) grain in eastern Ethiopia. Toxins 14: 473.https://doi.org/10.3390/toxins14070473
Muller, H.M. and Schwadorf, K., 1993. A survey of the natural occurrence ofFusarium toxins in wheat grown in south east of Germany. Mycopathologia 121: 115-121.
'A survey of the natural occurrence ofFusarium toxins in wheat grown in south east of Germany ' () 121 Mycopathologia : 115 -121.
Muthomi, J.W., Ndung’u, J.K., Gathumbi, J.K., Mutitu, E.W. and Wagacha, J.M., 2008. The occurrence ofFusarium species and mycotoxins in Kenyan wheat. Crop Protection 27: 1215-1219.
'The occurrence ofFusarium species and mycotoxins in Kenyan wheat ' () 27 Crop Protection : 1215 -1219.
Parry, D.W., Jenkinson, P. and McLeod, L., 1995.Fusarium ear blight (scab) in small grain cereals – a review. Plant Pathology 44: 207-238.
'Fusarium ear blight (scab) in small grain cereals – a review ' () 44 Plant Pathology : 207 -238.
Pitt, J., Wild, C., Baan, R., Gelderblom, W., Miller, J., Riley, R. and Wu, F., 2012. Improving public health through mycotoxin control. IARC Scientific Publication No. 158. IARC, Lyon, France.
'Improving public health through mycotoxin control', ().
Popovski, S. and Celar, F.A., 2013. The impact of environmental factors on the infection of cereals withFusarium species and mycotoxin production – a review. Acta Agriculturae Slovenica 101: 105-116.
'The impact of environmental factors on the infection of cereals withFusarium species and mycotoxin production – a review ' () 101 Acta Agriculturae Slovenica : 105 -116.
Samson, R.A., Houbraken, J., Thrane, U., Frisvad, J.C. and Andersen, B., 2010. Food and indoor fungi. Laboratory Manual Series. Fungal Biodiversity Center, Utrecht, the Netherlands.
'Food and indoor fungi', ().
Schaarschmidt, S. and Fauhl-Hassek, C., 2018. The fate of mycotoxins during the processing of wheat for human consumption. Comprehensive Reviews in Food Science and Food Safety 17: 556-593.
'The fate of mycotoxins during the processing of wheat for human consumption ' () 17 Comprehensive Reviews in Food Science and Food Safety : 556 -593.
Shiferaw, B., Kassie, M., Jaleta, M. and Yirga, C., 2014. Adoption of improved wheat varieties and impacts on household food security in Ethiopia. Food Policy 44: 272-284.
'Adoption of improved wheat varieties and impacts on household food security in Ethiopia ' () 44 Food Policy : 272 -284.
Spanic, V., Katanic, Z., Sulyok, M., Krska, R., Puskas, K., Vida, G., Drezner, G. and Šarkanj, B., 2020. Multiple fungal metabolites including mycotoxins in naturally infected andFusarium-inoculated wheat samples. Microorganisms 8: 578.
'Multiple fungal metabolites including mycotoxins in naturally infected andFusarium-inoculated wheat samples ' () 8 Microorganisms : 578.
Spanic, V., Marcek, T., Abicic, I. and Sarkanj, B., 2018. Effects ofFusarium head blight on wheat grain and malt infected byFusarium culmorum. Toxins 10: 17.
'Effects ofFusarium head blight on wheat grain and malt infected byFusarium culmorum ' () 10 Toxins : 17.
Sulyok, M., Stadler, D., Steiner, D. and Krska, R., 2020. Validation of an LC-MS/MS-based dilute-and-shoot approach for the quantification of >500 mycotoxins and other secondary metabolites in food crops: challenges and solutions. Analytical and Bioanalytical Chemistry 412: 2607-2620.
'Validation of an LC-MS/MS-based dilute-and-shoot approach for the quantification of >500 mycotoxins and other secondary metabolites in food crops: challenges and solutions ' () 412 Analytical and Bioanalytical Chemistry : 2607 -2620.
Sunic, K., Kovac, T., Loncaric, A., Babic, J., Sulyok, M., Krska, R., Drezner, G. and Spanic, V. 2021.Fusarium secondary metabolite content in naturally produced and artificially provoked FHB pressure in winter wheat. Agronomy 11: 2239.
'Fusarium secondary metabolite content in naturally produced and artificially provoked FHB pressure in winter wheat ' () 11 Agronomy : 2239.
Tadesse, W., Bishaw, Z. and Assefa, S., 2019. Wheat production and breeding in Sub-Saharan Africa: challenges and opportunities in the face of climate change. International Journal of Climate Change Strategies and Management 11: 696-715.
'Wheat production and breeding in Sub-Saharan Africa: challenges and opportunities in the face of climate change ' () 11 International Journal of Climate Change Strategies and Management : 696 -715.
Tebele, S.M., Gbashi, S., Adebo, O., Changwa, R., Naidu, K. and Njobeh, P.B., 2020. Quantification of multi-mycotoxin in cereals (maize, maize porridge, sorghum and wheat) from Limpopo province of South Africa. Food Additives and Contaminants Part A 37: 1922-1938.
'Quantification of multi-mycotoxin in cereals (maize, maize porridge, sorghum and wheat) from Limpopo province of South Africa ' () 37 Food Additives and Contaminants Part A : 1922 1938.
Udomkun, P., Wiredu, A.N., Nagle, M., Bandyopadhyay, R., Müller, J. and Vanlauwe, B., 2017. Mycotoxins in sub-Saharan Africa: present situation, socio-economic impact, awareness, and outlook. Food Control 72: 110-122.
'Mycotoxins in sub-Saharan Africa: present situation, socio-economic impact, awareness, and outlook ' () 72 Food Control : 110 -122.
Uhlig, S., Eriksen, G.S., Hofgaard, I.S., Krska, R., Beltrán, E. and Sulyok, M., 2013. Faces of a changing climate: semi-quantitative multi-mycotoxin analysis of grain grown in exceptional climatic conditions in Norway. Toxins 5: 1682-1697.
'Faces of a changing climate: semi-quantitative multi-mycotoxin analysis of grain grown in exceptional climatic conditions in Norway ' () 5 Toxins : 1682 -1697.
Valverde-Bogantes, E., Bianchini, A., Herr, J.R., Rose, D.J., Wegulo, S.N. and Hallen-Adams, H.E., 2020. Recent population changes ofFusarium head blight pathogens: drivers and implications. Canadian Journal of Plant Pathology 42: 315-329.
'Recent population changes ofFusarium head blight pathogens: drivers and implications ' () 42 Canadian Journal of Plant Pathology : 315 -329.
Wagacha, J.M., Steiner, U., Dehne, H.W., Zuehlke, S., Spiteller, M., Muthomi, J. and Oerke, E.C., 2010. Diversity in mycotoxins and fungal species infecting wheat in Nakuru District, Kenya. Journal of Phytopathology 158: 527-535.
'Diversity in mycotoxins and fungal species infecting wheat in Nakuru District, Kenya ' () 158 Journal of Phytopathology : 527 -535.
Waśkiewicz, A., Beszterda, M. and Goliński, P., 2012. Occurrence of fumonisins in food-an interdisciplinary approach to the problem. Food Control 26: 491-499.
'Occurrence of fumonisins in food-an interdisciplinary approach to the problem ' () 26 Food Control : 491 -499.
Worku, A., Merkuz, A., Kalsa, K., Tenagashaw, M. and Habtu, N., 2019. Occurrence of mycotoxins in farm-stored wheat in Ethiopia. African Journal of Food, Agriculture, Nutrition and Development 19: 14829-14847.
'Occurrence of mycotoxins in farm-stored wheat in Ethiopia ' () 19 African Journal of Food, Agriculture, Nutrition and Development : 14829 -14847.
Wu, F., 2004. Mycotoxin risk assessment for the purpose of setting international regulatory standards. Environmental Science and Technology 30: 4049-4055.
'Mycotoxin risk assessment for the purpose of setting international regulatory standards ' () 30 Environmental Science and Technology : 4049 -4055.
Yazar, S. and Omurtag, G.Z., 2008. Fumonisins, trichothecenes and zearalenone in cereals. International Journal of Molecular Sciences 9: 2062-2090.
'Fumonisins, trichothecenes and zearalenone in cereals ' () 9 International Journal of Molecular Sciences : 2062 -2090.
Zinedine, A., Meca, G., Mañes, J. and Font, G., 2011. Further data on the occurrence ofFusarium emerging mycotoxins enniatins (A, A1, B, and B1), fusaproliferin and beauvericin in raw cereals commercialized in Morocco. Food Control 22: 1-5.
'Further data on the occurrence ofFusarium emerging mycotoxins enniatins (A, A1, B, and B1), fusaproliferin and beauvericin in raw cereals commercialized in Morocco ' () 22 Food Control : 1 -5.
All Time | Past 365 days | Past 30 Days | |
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Ethiopia is sub-Saharan Africa’s greatest wheat producer. However, its production, productivity and utilisations are constrained by fungal infections, which lead to yield losses and mycotoxin contaminations. The present study was aimed to determined fungal species and multi-mycotoxins in wheat grain samples (n=178) collected during the 2020 cropping season in Ethiopia. Different fungal genera ofAlternaria,Aspergillus,Fusarium,Penicillium, andRhizopus species were isolated from the infected grain samples, of whichFusarium species were abundantly prevalent. Multi-mycotoxin analysis done using liquid chromatography-tandem mass spectrometric (LC-MS/MS). Overall, 49 metabolites of different fungal species including masked, regulated and emerging mycotoxins were detected and categorised into eight groups. Among major mycotoxins and derivatives, deoxynivalenol was frequently detected in about 70.8% of samples with the maximum concertation 15,900 μg/kg, followed by its masked metabolites of deoxynivalenol-3-glucoside detected in 64% of the samples with maximum level 2,120 μg/kg. AmongFusarium metabolites, 15-hydroxyculmorin was detected in 46.6% of wheat samples with maximum level of 4,290 μg/kg, while chrysogin, enniatin B and culmorin were detected in 58.4, 43.3 and 44.9%, of the wheat samples with maximum levels of 154, 325 and 2,860 μg/kg, respectively. AmongAlternaria metabolites tenuazonic acid was most prevalent mycotoxin occurring in 79.2% of samples with maximum level (236 μg/kg). Furthermore, metabolites from other fungal genera were detected at different levels. The widespread presence and high amount of multi-mycotoxin in wheat samples indicated proportionately higher exposure levels, which could result in serious food safety and health consequences. Integrating several management strategies both before and after harvest may lower the risk of mycotoxin contamination in wheat, being used as diversified food products in the country.
All Time | Past 365 days | Past 30 Days | |
---|---|---|---|
Abstract Views | 231 | 173 | 29 |
Full Text Views | 76 | 14 | 0 |
PDF Views & Downloads | 90 | 31 | 0 |