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An investigation of different antimycotoxin additives in swine intestinal explants challenged with aflatoxin and fumonisin: ex vivo and in vitro models

In: World Mycotoxin Journal
Authors:
J. Alves Sarturi Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Av. Roraima 1000, 97105-900, Santa Maria, RS, Brazil

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https://orcid.org/0000-0002-8869-0207
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C. Tonial Simões Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Av. Roraima 1000, 97105-900, Santa Maria, RS, Brazil

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https://orcid.org/0000-0002-7345-9440
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C. Rosa da Silva Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Av. Roraima 1000, 97105-900, Santa Maria, RS, Brazil

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https://orcid.org/0000-0001-7119-9588
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I. Fabris Laber Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Av. Roraima 1000, 97105-900, Santa Maria, RS, Brazil

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https://orcid.org/0000-0002-1470-3626
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L.M. de Lima Schlösser Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Av. Roraima 1000, 97105-900, Santa Maria, RS, Brazil

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https://orcid.org/0000-0001-8438-749X
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D.F. Sturza Samitec Institute – Microbiological and Technological Analytical Solutions, 97105-910, Santa Maria, RS, Brazil

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https://orcid.org/0000-0002-0656-0223
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C.A. Mallmann Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Av. Roraima 1000, 97105-900, Santa Maria, RS, Brazil

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https://orcid.org/0000-0003-2376-2705
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Abstract

This study aimed to develop an ex vivo model to evaluate the efficacy of antimycotoxin additives (AMAs) against aflatoxin B1 (AFB1) and fumonisin B1 (FB1) using intestinal explants of growing pigs. Four assays of two treatments with 12 replicates each (24 jejunal explants used per assay) were performed using an Ussing Chamber (UC) system: two assays to evaluate two AMAs for AFB1 and two assays to evaluate two AMAs for FB1. The difference between the two assays for both AFB1 and FB1 was the composition of the additive used. The treatments for AFB1 assays were control [Buffer solution (BS) + 1 mg/l AFB1] and AMA (BS + 1 mg/l AFB1 + 0.5% AMA 1 or 2). The treatments for FB1 assays were control (BS + 50 mg/l FB1) and AMA (BS + 50 mg/l FB1 + 0.5% AMA 3 or 4). The efficacy of the four additives was also tested in vitro. The AFB1 concentrations in the explants from AMAs 1 and 2 were lower ( P < 0.0001) than in the control. AMAs 1 and 2 reduced the jejunal absorption of AFB1 by 83.4 and 72.9%, respectively. Explants from AMAs 3 and 4 had lower FB1 ( P < 0.0001) concentration when compared to the respective control treatment. AMAs 3 and 4 reduced the FB1 absorption by 31.9 and 17.6%, respectively. In the in vitro test, AMAs 1 and 2 provided 98.4 and 86.3% of AFB1 adsorption, respectively, while AMA 3 and 4 provided 91.2 and 80.5% FB1 adsorption, respectively. The ex vivo model can be a useful tool in evaluating the effectiveness of antimycotoxin additives for AFB1 and FB1 in swine. However, the low FB1 uptake in jejunal explants highlights the need for the development of additional information to improve the method.

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