A rapid, selective and reliable sample preparation technique employing solid-phase extraction (SPE) based on molecularly imprinted polymer (MIP) for the determination of ochratoxin A (OTA) in human urine was described. After sample adjustment to pH 2.5 with 0.1 M HCl, the urine sample was loaded onto the MIP-SPE column, and after a wash step, OTA was eluted for measurement by ultra-high performance liquid chromatography coupled with fluorescence detection. Key parameters which affected the MIP-SPE extraction efficiency were optimized as was the detection method. Under the optimised conditions, the limits of detection and quantification for OTA in urine were 0.2 ng/ml and 0.6 ng/ml, respectively. The recoveries for OTA in urine, spiked at the 0.6, 6.0 and 60 ng/ml levels, ranged from 92.0 to 98.9%. Sixty urine samples were analysed, of which four were found to contain OTA at concentrations ranging from 0.022 to 0.083 ng/ml; the positive results were confirmed by liquid chromatography coupled with tandem mass spectrometry. OTA determination in urine is a good indicator for human exposure to the mycotoxin, and this is the first report on OTA contamination in Chinese people.
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Ali, W.H., Derrien, D., Alix, F., Pérollier, C., Lépine, O., Bayoudh, S., Chapuis-Hugon, F. and Pichon, V., 2010. Solid-phase extraction using molecularly imprinted polymers for selective extraction of a mycotoxin in cereals. Journal of Chromatography A 1217: 6668-6673.
'Solid-phase extraction using molecularly imprinted polymers for selective extraction of a mycotoxin in cereals ' () 1217 Journal of Chromatography A : 6668 -6673.
Biasucci, G., Calabrese, G., Giuseppe, R.D., Carrara, G., Colombo, F., Mandelli, B., Maj, M., Bertuzzi, T., Pietri, A. and Rossi, F., 2011. The presence of ochratoxin A in cord serum and in human milk and its correspondence with maternal dietary habits. European Journal of Clinical Nutrition 50: 211-218.
'The presence of ochratoxin A in cord serum and in human milk and its correspondence with maternal dietary habits ' () 50 European Journal of Clinical Nutrition : 211 -218.
Blesa, J., Berrada, H., Soriano, J.M., Moltó, J.C. and Mañes, J., 2004. Rapid determination of ochratoxin A in cereals and cereal products by liquid chromatography. Journal of Chromatography A 1046: 127-131.
'Rapid determination of ochratoxin A in cereals and cereal products by liquid chromatography ' () 1046 Journal of Chromatography A : 127 -131.
Cao, J.L., Kong, W.J., Zhou, S.J., Yin, L.H., Wan, L. and Yang, M.H., 2013. Molecularly imprinted polymer-based solid phase clean-up for analysis of ochratoxin A in beer, red wine and grape juice. Journal of Separation Science 36: 1291-1297.
'Molecularly imprinted polymer-based solid phase clean-up for analysis of ochratoxin A in beer, red wine and grape juice ' () 36 Journal of Separation Science : 1291 -1297.
Domijan, A.M., Peraica, M., Miletić-Medved, M., Lucić, A. and Fuchs, R., 2003. Two different clean-up procedures for liquid chromatographic determination of ochratoxin A in urine. Journal of Chromatography B 798: 317-321.
'Two different clean-up procedures for liquid chromatographic determination of ochratoxin A in urine ' () 798 Journal of Chromatography B : 317 -321.
Duarte, S.C., Alves, M.R., Pena, A. and Lino, C.M., 2012. Determinants of ochratoxin A exposure-A one year follow-up study of urine levels. International Journal of Hygiene and Environmental Health 215: 360-367.
'Determinants of ochratoxin A exposure-A one year follow-up study of urine levels ' () 215 International Journal of Hygiene and Environmental Health : 360 -367.
European Commission (EC), 2002. Commission Directive 2002/26/ EC of 13 March 2002 laying down the sampling methods and the methods of analysis for the official control of the levels of ochratoxin A in foodstuffs. Official Journal of the European Union L75: 38-43.
European Commission (EC), 2006. Commission Regulation (EC) no. 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Union L364: 5-24.
European Commission (EC), 2010. Commission Regulation (EC) no. 105/2010 of 5 February 2010 amending Regulation (EC) No. 1881/2006 setting maximum levels for certain contaminants in foodstuffs as regards ochratoxin A. Official Journal of the European Union L35: 7-8.
European Food Safety Authority (EFSA), 2006. Question no EFSA. Q-2005-154. Opinion of the scientific panel on contaminants in the food chain on a request from the commission related to ochratoxin A in food. EFSA Journal 365: 1-56.
Gekle, M., Sauvant, C. and Schwerdt, G., 2005. Ochratoxin A at nanomolar concentrations: a signal modular in renal cells. Molecular Nutrition and Food Research 49: 118-130.
'Ochratoxin A at nanomolar concentrations: a signal modular in renal cells ' () 49 Molecular Nutrition and Food Research : 118 -130.
Gilbert, J., Brereton, P. and MacDonald, S., 2001. Assessment of dietary exposure to ochratoxin A in the UK using a duplicate diet approach and analysis of urine and plasma samples. Food Additives and Contaminants 18: 1088-1093.
'Assessment of dietary exposure to ochratoxin A in the UK using a duplicate diet approach and analysis of urine and plasma samples ' () 18 Food Additives and Contaminants : 1088 -1093.
González-Osnaya, L., Soriano, J.M., Moltó, J.C. and Mañes, J., 2008. Simple liquid chromatography assay for analyzing ochratoxin A in bovine milk. Food Chemistry 108: 272-276.
'Simple liquid chromatography assay for analyzing ochratoxin A in bovine milk ' () 108 Food Chemistry : 272 -276.
González-Peñas, E., Leache, C., Viscarret, M., Pérez de Obanos, A., Araguás, C. and López de Cerain, A., 2004. Determination of ochratoxin A in wine using liquid-phase microextraction combined with liquid chromatography with fluorescence detection. Journal of Chromatography A 1025: 163-168.
'Determination of ochratoxin A in wine using liquid-phase microextraction combined with liquid chromatography with fluorescence detection ' () 1025 Journal of Chromatography A : 163 -168.
Goryacheva, I.Y., Saeger, S.D., Nesterenko, I.S., Eremin, S.A. and Peteghem, C.V., 2007. Rapid all-in-one three-step immunoassay for non-instrumental detection of ochratoxin A in high-coloured herbs and spices. Talanta 72: 1230-1234.
'Rapid all-in-one three-step immunoassay for non-instrumental detection of ochratoxin A in high-coloured herbs and spices ' () 72 Talanta : 1230 -1234.
International Agency for Research on Cancer (IARC), 1993. IARC Monographs on the Evaluation of Carcinogenic Risk to Humans, volume 56. Some naturally occurring substances: food items and constituents, heterocyclic aromatic amines and mycotoxins. IARC, Lyon, France, 489 pp.
Jodlbauer, J., Maier, N.M. and Lindner, W., 2002. Towards ochratoxin A selective molecularly imprinted polymers for solid-phase extraction. Journal of Chromatography A 945: 45-63.
'Towards ochratoxin A selective molecularly imprinted polymers for solid-phase extraction ' () 945 Journal of Chromatography A : 45 -63.
Joint FAO/WHO Expert Committee on Food Additives (JEFCA), 2001. Safety evaluation of certain mycotoxins in food. WHO Food Additives Series 47, FAO Food and Nutrition Paper 74. WHO, Geneva, Switzerland.
Kong, W.J., Xie, T.T., Li, J.Y., Wei, J.H., Qiu, F., Qi, A.D., Zheng, Y.G. and Yang, M.H., 2012. Analysis of fumonisins B1 and B2 in spices and aromatic and medicinal herbs by HPLC-FLD with on-line post-column derivatization and positive confirmation by LC-MS/ MS. Analyst 137: 3166-3174.
'Analysis of fumonisins B1 and B2 in spices and aromatic and medicinal herbs by HPLC-FLD with on-line post-column derivatization and positive confirmation by LC-MS/ MS ' () 137 Analyst : 3166 -3174.
Lee, T.P., Saad, B., Khayoon, W.S. and Salleh, B., 2012. Molecularly imprinted polymer as sorbent in micro-solid phase extraction of ochratoxin A in coffee, grape juice and urine. Talanta 88: 129-135.
'Molecularly imprinted polymer as sorbent in micro-solid phase extraction of ochratoxin A in coffee, grape juice and urine ' () 88 Talanta : 129 -135.
Lucci, P., Derrien, D., Alix, F., Pérollier, C. and Bayoudh, S., 2010. Molecularly imprinted polymer solid-phase extraction for detection of zearalenone in cereal sample extracts. Analytica Chimica Acta 672: 15-19.
'Molecularly imprinted polymer solid-phase extraction for detection of zearalenone in cereal sample extracts ' () 672 Analytica Chimica Acta : 15 -19.
Maaroufi, K., Achour, A., Betbeder, A. M., Hammami, M., Ellouz, F., Creppy, E.E. and Bacha, H., 1995a. Foodstuffs and human blood contamination by the mycotoxin ochratoxin A: correlation with chronic interstitial nephropathy in Tunisia. Archives of Toxicology 69: 552-558.
'Foodstuffs and human blood contamination by the mycotoxin ochratoxin A: correlation with chronic interstitial nephropathy in Tunisia ' () 69 Archives of Toxicology : 552 -558.
Maaroufi, K., Achour, A., Hammami, M., May, M.E., Betbeder, A.M, Ellouz, F., Creppy, E.E. and Bacha, H., 1995b. Ochratoxin A in human blood in relation to nephropathy in Tunisia. Human and Experimental Toxicology 14: 609-615.
'Ochratoxin A in human blood in relation to nephropathy in Tunisia ' () 14 Human and Experimental Toxicology : 609 -615.
Maier, N.M., Buttinger, G., Welhartizki, S., Gavioli, E. and Lindner, W., 2004. Molecularly imprinted polymer-assisted sample clean-up of ochratoxin A from red wine: merits and limitations. Journal of Chromatography B 804: 103-111.
'Molecularly imprinted polymer-assisted sample clean-up of ochratoxin A from red wine: merits and limitations ' () 804 Journal of Chromatography B : 103 -111.
Muñoz, K., Blaszkewicz, M. and Degen, G.H., 2010. Simultaneous analysis of ochratoxin A and its major metabolite ochratoxin alpha in plasma and urine for an advanced biomonitoring of the mycotoxin. Journal of Chromatography B 878: 2623-2629.
'Simultaneous analysis of ochratoxin A and its major metabolite ochratoxin alpha in plasma and urine for an advanced biomonitoring of the mycotoxin ' () 878 Journal of Chromatography B : 2623 -2629.
Owens, P.K., Karlsson, L., Lutz, E.S.M. and Andersson, L.I., 1999. Molecular imprinting for bio-and pharmaceutical analysis. Trends in Analytical Chemistry 18: 146-154.
'Molecular imprinting for bio-and pharmaceutical analysis ' () 18 Trends in Analytical Chemistry : 146 -154.
Pfohl-Leszkowicz, A. and Manderville, R.A., 2007. Ochratoxin A: an overview on toxicity and carcinogenicity in animals and humans. Molecular Nutrition and Food Research 51: 61-99.
'Ochratoxin A: an overview on toxicity and carcinogenicity in animals and humans ' () 51 Molecular Nutrition and Food Research : 61 -99.
Pfohl-Leszkowicz, A., Petkova-Bocharova, T., Chernozemsky, I.N. and Castegnaro, M., 2002. Balkan endemic nephropathy and associated urinary tract tumours: a review on aetiological causes and the potential role of mycotoxins. Food Additives and Contaminants 19: 282-302.
'Balkan endemic nephropathy and associated urinary tract tumours: a review on aetiological causes and the potential role of mycotoxins ' () 19 Food Additives and Contaminants : 282 -302.
Radić, B., Fuchs, R., Peraica, M. and Lucić, A., 1997. Ochratoxin A in human sera in the area with endemic nephropathy in Croatia. Toxicology Letters 91: 105-109.
'Ochratoxin A in human sera in the area with endemic nephropathy in Croatia ' () 91 Toxicology Letters : 105 -109.
Reiter, E.V., Cichna-Markl, M., Chung, D.H., Shim, W.B., Zentek, J. and Razzazi-Fazeli, E., 2011. Determination of ochratoxin A in grains by immuno-ultrafiltration and HPLC-fluorescence detection after postcolumn derivatisation in an electrochemical cell. Analytical and Bioanalytical Chemistry 400: 2615-2622.
'Determination of ochratoxin A in grains by immuno-ultrafiltration and HPLC-fluorescence detection after postcolumn derivatisation in an electrochemical cell ' () 400 Analytical and Bioanalytical Chemistry : 2615 -2622.
Sáez, J.M., Medina, Á., Gimeno-Adelantado, J.V., Mateo, R. and Jiménez, M., 2004. Comparison of different sample treatments for the analysis of ochratoxin A in must, wine and beer by liquid chromatography. Journal of Chromatography A 1029: 125-133.
'Comparison of different sample treatments for the analysis of ochratoxin A in must, wine and beer by liquid chromatography ' () 1029 Journal of Chromatography A : 125 -133.
Sangare-Tigori, B., Moukha, S., Kouadio, J.H., Dano, D.S., Betbeder, A.M., Achour, A. and Creppy, E.E., 2006. Ochratoxin A in human blood in Abidjan, Côte d’Ivoire. Toxicon 47: 894-900.
'Ochratoxin A in human blood in Abidjan, Côte d’Ivoire ' () 47 Toxicon : 894 -900.
Stoev, S.D., 1998. The role of ochratoxin A as a possible cause of Balkan endemic nephropathy and its risk evaluation. Veterinary and Human Toxicology 40: 352-360.
'The role of ochratoxin A as a possible cause of Balkan endemic nephropathy and its risk evaluation ' () 40 Veterinary and Human Toxicology : 352 -360.
Thirumala-Devi, K., Mayo, M.A., Reddy, G., Emmanuel, K.E., Larondelle, Y. and Reddy, D.V.R., 2001. Occurrence of ochratoxin A in black pepper, coriander, ginger and turmeric in India. Food Additives and Contaminants 18: 830-835.
'Occurrence of ochratoxin A in black pepper, coriander, ginger and turmeric in India ' () 18 Food Additives and Contaminants : 830 -835.
Trucksess, M., Weaver, C., Oles, C., D’Ovidio, K. and Rader, J., 2006. Determination of aflatoxins and ochratoxin A in Ginseng and other botanical roots by immunoaffinity column cleanup and liquid chromatography with fluorescence detection. Journal of AOAC International 89: 624-630.
'Determination of aflatoxins and ochratoxin A in Ginseng and other botanical roots by immunoaffinity column cleanup and liquid chromatography with fluorescence detection ' () 89 Journal of AOAC International : 624 -630.
Valenta, H., 1998. Chromatographic methods for the determination of ochratoxin A in animal and human tissues and fluids. Journal of Chromatography A 815: 75-92.
'Chromatographic methods for the determination of ochratoxin A in animal and human tissues and fluids ' () 815 Journal of Chromatography A : 75 -92.
Vatinno, R., Aresta, A., Zambonin, C.G. and Palmisano, F., 2007. Determination of ochratoxin A in human urine by solid-phase microextraction coupled with liquid chromatography-fluorescence detection. Journal of Pharmaceutical and Biomedical Analysis 44: 1014-1018.
'Determination of ochratoxin A in human urine by solid-phase microextraction coupled with liquid chromatography-fluorescence detection ' () 44 Journal of Pharmaceutical and Biomedical Analysis : 1014 -1018.
Vatinno, R., Vuckovic, D., Zambonin, C.G. and Pawliszyn, J., 2008. Automated high-throughput method using solid-phase microextraction-liquid chromatography-tandem mass spectrometry for the determination of ochratoxin A in human urine. Journal of Chromatography A 1201: 215-221.
'Automated high-throughput method using solid-phase microextraction-liquid chromatography-tandem mass spectrometry for the determination of ochratoxin A in human urine ' () 1201 Journal of Chromatography A : 215 -221.
Visconti, A., Pascale, M. and Centonze, G., 2000. Determination of ochratoxin A in domestic and imported beers in Italy by immunoaffinity clean-up and liquid chromatography. Journal of Chromatography A 888: 321-326.
'Determination of ochratoxin A in domestic and imported beers in Italy by immunoaffinity clean-up and liquid chromatography ' () 888 Journal of Chromatography A : 321 -326.
Zimmerli, B. and Dick, R., 1995. Determination of ochratoxin A at the ppt level in human blood, serum, milk and some foodstuffs by high performance liquid chromatography with enhanced fluorescence detection and immunoaffinity column clean-up: methodology and Swiss data. Journal of Chromatography B 666: 85-99.
'Determination of ochratoxin A at the ppt level in human blood, serum, milk and some foodstuffs by high performance liquid chromatography with enhanced fluorescence detection and immunoaffinity column clean-up: methodology and Swiss data ' () 666 Journal of Chromatography B : 85 -99.
All Time | Past 365 days | Past 30 Days | |
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Abstract Views | 178 | 149 | 11 |
Full Text Views | 29 | 1 | 0 |
PDF Views & Downloads | 11 | 1 | 0 |
A rapid, selective and reliable sample preparation technique employing solid-phase extraction (SPE) based on molecularly imprinted polymer (MIP) for the determination of ochratoxin A (OTA) in human urine was described. After sample adjustment to pH 2.5 with 0.1 M HCl, the urine sample was loaded onto the MIP-SPE column, and after a wash step, OTA was eluted for measurement by ultra-high performance liquid chromatography coupled with fluorescence detection. Key parameters which affected the MIP-SPE extraction efficiency were optimized as was the detection method. Under the optimised conditions, the limits of detection and quantification for OTA in urine were 0.2 ng/ml and 0.6 ng/ml, respectively. The recoveries for OTA in urine, spiked at the 0.6, 6.0 and 60 ng/ml levels, ranged from 92.0 to 98.9%. Sixty urine samples were analysed, of which four were found to contain OTA at concentrations ranging from 0.022 to 0.083 ng/ml; the positive results were confirmed by liquid chromatography coupled with tandem mass spectrometry. OTA determination in urine is a good indicator for human exposure to the mycotoxin, and this is the first report on OTA contamination in Chinese people.
All Time | Past 365 days | Past 30 Days | |
---|---|---|---|
Abstract Views | 178 | 149 | 11 |
Full Text Views | 29 | 1 | 0 |
PDF Views & Downloads | 11 | 1 | 0 |