The article studies agricultural science teaching and research in the Napoleonic Kingdom of Italy. In particular, it considers the three national universities of Pavia, Bologna and Padua, highlighting both the points in common and the differences between them. It analyses both unpublished documentation and monographs, dissertations, scientific journals circulating at the time. The article is introduced by an analysis of the Napoleonic legislation for the strengthening of agricultural science as an institutional knowledge. The study related to the University of Pavia comes next. It isolates the topics of cultivation of cereals and grain conservation techniques in order to analyse the educational and scientific activity and its contribution to the national debate. This is followed by an analysis of the professorships in Bologna and Padua, considering similarities and differences with Pavia in matter of topics.