We report on the results of small-scale excavations at the archaeological site of Kalambo Falls, northern Zambia. The site has long been known for its stratified succession of Stone Age horizons, in particular those representing the late Acheulean (Mode 2) and early Middle Stone Age (Mode 3). Previous efforts to date these horizons have provided, at best, minimum radiometric ages. The absence of a firm chronology for the site has limited its potential contribution to our understanding of the process of technological change in the Middle Pleistocene of south-central Africa. The aim of the excavations was to collect samples for luminescence dating that bracketed archaeological horizons, and to establish the sedimentary and palaeoenvironmental contexts of the deposits. Four sedimentary packages were identified with the oldest containing Mode 2 and Mode 3 horizons. In this paper we consider the implications of the luminescence ages for the archaeological record at Kalambo Falls, and place them in a regional context. The reworking and preservation of the archaeological horizons is interpreted as the result of successive phases of meander migration and aggradation. Limited pollen evidence suggests a persistent floodplain palaeoenvironment with intermittent swamp forest and adjacent valley woodland, while mineral magnetic susceptibility data support an interpretation of river flow variability without any significant change in sediment provenance. The dynamics of the fluvial system cannot as yet be linked directly with regional climate change. The age range of ~500–300 ka for the oldest sedimentary package places the Mode 2/3 succession firmly in the Middle Pleistocene, and contributes to an expanding African record of technological innovation before the evolution of Homo sapiens.