In Euripides’ Hecuba, both the scholia and modern interpreters detect a failure of communication in the farewell scene between the protagonist and Polyxena—though the scholiast names Polyxena as the source of the non-dialogue, whereas the modern commentators claim that neither character is engaging. This paper aims, firstly, by a slight redistribution of lines, to restore coherence to the dialogue. Secondly, it argues that it is Hecuba’s rather than Polyxena’s conversational behaviour that impedes the smooth progress of the dialogue. Polyxena is even the one trying to reintegrate her mother into the dialogue. Her linguistic behaviour thus matches her composed and ‘heroic’ overall conduct.
The structural genes of the nitrogenase enzyme complex of Rhizobium japonicum were isolated from the total genome of this organism using recombinant DNA technology. A recombinant plasmid, pRJ676, was thus found in a clone bank. It contains a DNA region that has homology with the Klebsiella pneumoniae genes nifH and nifD, which code for two of the three polypeptides of the nitrogenase complex. The expression of several subfragments of pRJ676 was tested by cloning them into suitable vectors in such a way that they were transcribed from the strong promoters of antibiotic resistance genes. Transposon (Tn5) insertion mutations were used to determine the exact end points of transcriptional units. We postulate the existence of a transcriptional unit that is large enough to accomodate all three nitrogenase structural genes.