Understanding the occurrence and distribution of parasitic nematodes is crucial – some are economic pests, and some are important predators of their insect associates. In our recent nematode inventory survey, two populations of an ektaphelechid nematode were detected in the branches of Pinus trees; later the same nematode population was detected in plant quarantine examinations of wood packaging material imported from Japan. The species was processed and identified as Ektaphelenchoides diversislocis sp. n. The new species can be characterised by having three lateral lines, stylet long and tripartite, excretory pore at the level of nerve ring, comparatively longer post-vulval uterine sac, indistinct rectum and anus. Female posterior body region conical, gradually narrowing, like a mucron or filiform. Male spicule with well-developed condylus, triangular rostrum, and cucullus absent. The species is morphologically and molecularly close to E. compsi. The new species was characterised with near full-length 18S, 28S D2-D3 regions, and internal transcribed spacer (ITS) rRNA genes and mitochondrial cytochrome oxidase I (COI) gene sequences. The species within the Ektaphelenchoides genus are considered to have predatory roles against insect associates; therefore, the discovery of E. diversislocis sp. n. from local and foreign woodlands warrants increased sampling and research attention.