Jaroslav Piálek and Peter Mikulíček
Vit Zavadil, Lilian Klepsch and Jaroslav Piálek
Three populations of the Triturus cristatus superspecies were studied using morphological and electrophoretic techniques. Two bivariate statistics - the ratio between the length of forelimbs and interlimb distances and that between head width and body length - and eleven allozyme loci revealed close similarity between a sample taken in Moravia (Czech Republic) and a sample of T. dobrogicus collected in Vienna (Austria). Accordingly, the Moravian population should be classified as T dobrogicus. Therewith, the northeastern limit of the known distribution of Triturus dobrogicus is extended into the Czech Republic.
Peter Mikulícek, Jelka Crnobrnja-Isailovic and Jaroslav Piálek
Theoretical and empirical studies revealed that fast evolving microsatellite markers might be successfully employed in phylogenetic reconstruction. In this study we used seven highly polymorphic microsatellite loci and six different genetic distances to infer the performance of microsatellites in estimation of phylogenetic relationships between closely related crested newt species (Triturus cristatus superspecies). Many intraspecific distances exceeded interspecific values likely due to loss of genetic distance linearity in time, revealing that none of them was able to estimate divergence between the species. The Neighbor-joining trees constructed on the basis of genetic distances showed trichotomies (three-way polychotomies) and low bootstrap support at the species level. This study thus revealed limits of microsatellites to estimate phylogenetic relationships between the crested newt taxa.