Two new species of Dorylaimidae from Iran are described using morphology and molecular data. Dorylaimus elegans sp. n. is characterised by 4.41-5.92 mm body length, lip region truncate, distinct from adjoining body by a depression, cuticle with 50-55 longitudinal ridges, odontostyle 42-51 μm long, thicker than cuticle at its level, didelphic female reproductive apparatus, vulva a small sub-equatorial pore, a variable number of advulval papillae (0-6), both pre- and post-vulval, present in most specimens, female tail elongate, 5.6-7.6 anal body diam. long, with ventrally hooked terminus. Male with 26-30 ventromedian supplements arranged very close to each other, copulatory hump weakly developed, and a convex-conoid tail with blunt terminus. Prodorylaimus reyesi sp. n. is characterised by 2.78-3.33 mm body length, lip region slightly angular and well set off from adjoining body by a depression, odontostyle 26-31 μm long, thicker than cuticle at its level, didelphic female reproductive apparatus, vulva longitudinal, a variable number of advulval papillae (0-3), both pre- and post-vulval, present in most specimens, tail in both sexes convex-conoid in its anterior part, then narrowing abruptly to a filiform hyaline appendix, longer in female (c′ = 11.7-15.5) than in male (c′ = 7-7.7), and 19-21 contiguous ventromedian supplements. A compendium of the main morphometric characters of Dorylaimus and a key to species are also provided.
During a nematological survey of the family Tripylidae, two new and two known species of the genus Tripyla, namely T. paraffinis sp. n., T. parafilicaudata sp. n., T. glomerans and T. setifera, were identified and described from soil samples collected from the rhizosphere of forest trees in northern Iran. Three populations of T. paraffinis sp. n., found in different locations, are described and morphometric data of the type and other populations provided. Tripyla paraffinis sp. n. is characterised by its body length of 1.21-1.89 mm, dorsal tooth wedge-shaped and triangular, short outer labial and cephalic sensilla, tail bent ventrad and gradually tapering to the end, horn-shaped spicules bearing a distinct constriction in the middle, and presence of 16-20 ventromedian supplementary papillae. Tripyla parafilicaudata sp. n. is described and illustrated from four different locations. It is characterised by females with a body length of 1.48-1.95 mm, dorsal tooth hook-shaped, vaginal wall with a downward pointing tooth-like projection in the middle, long outer labial and setiform cephalic sensilla, long tail, sausage-shaped spermatozoa, males with horn-shaped spicules and 11-17 ventromedian supplementary papillae. Tripyla glomerans and T. setifera are new records for the Iranian nematofauna. The results of phylogenetic analyses based on sequences of the partial 18S and D2-D3 expansion segments of 28S rRNA from T. paraffinis sp. n. and T. parafilicaudata sp. n. and other species in the genus clearly support the proposal of T. paraffinis sp. n. and T. parafilicaudata sp. n. as new species, as well as indicating that Tripyla shares a more recent common ancestor with Tobrilus, Tripylella, Prismatolaimus, Diphtherophora and two trichodorids, Trichodorus and Paratrichodorus. The Tripylidae is placed in a main clade within the Triplonchida.
A new bisexual species of Rotylenchus from North-western Iran is described and illustrated based on morphological, morphometric and molecular studies. Rotylenchus urmiaensis n. sp. is characterised by having a truncate lip region with irregular longitudinal striation, lateral field areolated only in pharynx region, stylet length 34-40 μm, vulva positioned at 53-61%, and female tail conoid-rounded to dorsally convex-conoid with 5-10 annuli. Rotylenchus urmiaensis n. sp. appears to be similar to R. striaticeps, from which it may be differentiated morphologically by a slightly shorter body length (870-1269 vs 1000-1723 μm), shorter stylet (34-40 vs 39-50 μm), female tail shape (conoid-rounded to dorsally convex-conoid vs rounded), frequency of males (rare vs common as abundant as females), shorter spicules (39-43 vs 41-50 μm) and phasmid position (varying from three annuli anterior or three annuli posterior to anus vs at level to seven annuli anterior to anus), and molecularly. The results of phylogenetic analyses based on sequences of D2-D3 expansion region of 28S, ITS-rDNA, 18S rDNA, and the partial cytochrome c oxidase subunit 1 (coxI) mtDNA, confirmed the species differentiation and the close molecular relationship between R. urmiaensis n. sp. and R. striaticeps.