The widespread use of ochre during Oxygen Isotope Stage 5 and 4 in South Africa has often been interpreted as reflecting complex behaviours amongst modern human populations. The Howiesons Poort is one of the most documented techno-complexes identified within this timeframe. It is associated with an intensification of a combination of innovative technical and symbolic behaviours. Despite the notable focus on ochre use, detailed analyses of Howiesons Poort assemblages in this respect are rare. New data on ochre exploitation from the Howiesons Poort of Klasies River main site are presented in this paper. We used non-destructive microscopic, colorimetric and chemical analyses (sem-eds, xrd) in order to describe the raw materials and the transformation of a selected sample from the Singer and Wymer ochre collection. This sample is composed of red and yellow ferruginous rocks (shale, ferricrete, siltstone and sandstone), along with whitish lumps (calcium phosphates). These lumps may have an anthropogenic origin and may be considered as pigments. Some of the red ochre pieces were probably deliberately heated. Our results enhance the impression of complexity emerging from the technical processes mastered by Howiesons Poort populations. Comparison with other Howiesons Poort ochre assemblages allows a discussion of regional variability and ‘connections’ between the sites. The scale and organization of social interactions in the Howiesons Poort are questioned.