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Summary

Rapid diagnosis tools for detection of root-knot nematodes play an important role in the disease control and eradication programme. Recombinase polymerase amplification (RPA) assays were developed targeting the IGS rRNA gene of the pacara earpod tree root-knot nematode, Meloidogyne enterolobii. The RPA assays using TwistAmp® Basic and TwistAmp® exo kits allowed detection of M. enterolobii from gall tissues and crude nematode extracts of all stages of target species without a DNA extraction step. The results of real-time RPA assays using a real-time fluorescent detection of a series of crude nematode extracts showed reliable detection with sensitivity of 1/10 of a second-stage juvenile in a RPA reaction tube after 15-20 min. The RPA assay provides affordable, simple, fast and sensitive detection of M. enterolobii.

Open Access
In: Nematology
The sheath nematodes belonging to the superfamily Hemicycliophoroidea are unique amongst all plant parasitic nematodes known to man due to the presence of an extra cuticular covering or sheath over the inner cuticle and body of all juvenile and adult life stages. These plant-parasitic nematodes include species of agricultural and quarantine importance.
In Systematics of the Sheath Nematodes of the Superfamily Hemicycliophoroidea John Chitambar and Sergei Subbotin provide a detailed review of the taxonomy and diagnosis of the superfamily, its member genera and 153 related species based on their morphological and molecular analyses, as well as a further understanding of the relationships within the superfamily using molecular phylogenetics. In addition, Chitambar and Subbotin also give detailed information on the global distribution, biology, host-parasite relationships and ecology of sheath nematodes.
In: Advances in Entomopathogenic Nematode Taxonomy and Phylogeny
In: Advances in Entomopathogenic Nematode Taxonomy and Phylogeny
Volume 8 in the series, appearing in two parts, A and B, deals with the systematics of cyst nematodes of the subfamily Heteroderinae. Cyst nematodes are important pathogens causing extensive damage and significant yield loss to many crops in both temperate and tropical regions. Part A presents summarised information on various aspects of morphology, biology, ecology, pathology, biogeography, control measures and diagnostics of these nematodes. Molecular diagnostic tables and comprehensive tabular and dichotomous keys for species identification, together with descriptions of protocols for extraction, light and electron microscopy studies, and molecular diagnostics are also included. The taxonomic section of part A provides diagnoses for six genera, viz., Globodera, Punctodera, Cactodera, Dolichodera, Betulodera and Paradolichodera, and includes descriptions and morphometrics of 30 valid species. The work is illustrated by 101 drawings and photos. The genus Heterodera is mainly covered in part B, complete descriptions and morphometrics of the 80 known valid species being provided and the species illustrated by 159 line drawings and photos. Identification of the species using morphological and molecular techniques is also covered. Each part includes an extensive bibliography.
Volume 8 in the series, appearing in two parts, A and B, deals with the systematics of cyst nematodes of the subfamily Heteroderinae. Cyst nematodes are important pathogens causing extensive damage and significant yield loss to many crops in both temperate and tropical regions. Part A presents summarised information on various aspects of morphology, biology, ecology, pathology, biogeography, control measures and diagnostics of these nematodes. Molecular diagnostic tables and comprehensive tabular and dichotomous keys for species identification, together with descriptions of protocols for extraction, light and electron microscopy studies, and molecular diagnostics are also included. The taxonomic section of part A provides diagnoses for six genera, viz., Globodera, Punctodera, Cactodera, Dolichodera, Betulodera and Paradolichodera, and includes descriptions and morphometrics of 30 valid species. The work is illustrated by 101 drawings and photos. The genus Heterodera is mainly covered in part B, complete descriptions and morphometrics of the 80 known valid species being provided and the species illustrated by 159 line drawings and photos. Identification of the species using morphological and molecular techniques is also covered. Each part includes an extensive bibliography.
Volume 8 in the series, appearing in two parts, A and B, deals with the systematics of cyst nematodes of the subfamily Heteroderinae. Cyst nematodes are important pathogens causing extensive damage and significant yield loss to many crops in both temperate and tropical regions. Part A presents summarised information on various aspects of morphology, biology, ecology, pathology, biogeography, control measures and diagnostics of these nematodes. Molecular diagnostic tables and comprehensive tabular and dichotomous keys for species identification, together with descriptions of protocols for extraction, light and electron microscopy studies, and molecular diagnostics are also included. The taxonomic section of part A provides diagnoses for six genera, viz., Globodera, Punctodera, Cactodera, Dolichodera, Betulodera and Paradolichodera, and includes descriptions and morphometrics of 30 valid species. The work is illustrated by 101 drawings and photos. The genus Heterodera is mainly covered in part B, complete descriptions and morphometrics of the 80 known valid species being provided and the species illustrated by 159 line drawings and photos. Identification of the species using morphological and molecular techniques is also covered. Each part includes an extensive bibliography.
Root-knot nematodes of the genus Meloidogyne represent one of the most damaging and agricultural important group of plant-parasitic nematodes. These nematodes are obligate sedentary endoparasites infecting most species of higher plants and have a cosmopolitan distribution. Annual worldwide economic losses due to nematode infection of crops have been estimated at several hundred billion US dollars. This book is the first complete illustrated compendium of root-knot nematode species and contains 98 species descriptions with comprehensive diagnoses, information on biology, plant-hosts, pathogenicity, symptoms, distribution and biochemical and molecular diagnostics. It also includes introductions into morphology, biology, biogeography, genomics, phylogeny and host-parasite relationships of root-knot nematodes.