Sensory Neuron , Vol. 3, No. 2, pp. 119– 125 (2000) Ó VSP 2000. Effects of amiloride and W-7 on the chemoreception of NaCl and KCl in the blow y: implications for the salt quality A. LISCIA ¤ , P. SOLARI and R. CRNJAR Dipartimento di Biologia Sperimentale, Sezione di Fisiologia Generale

In: Sensory Neuron

Prim. Sensory Neuron , Vol. 3, No. 1, pp. 49– 59 (1998) Ó VSP 1998. Amiloride high- and low-sensitivity, as well as insensitive sites in the blow y: implications for sugar, water and anion taste reception mechanisms A. LISCIA ¤ , P. SOLARI, R. MAJONE and R. CRNJAR Dipartimento di Biologia

In: Primary Sensory Neuron

Abstract

The issue that has divided opinion over the status of umami as a basic taste quality is its relationship with the taste of sodium salt. That is addressed here, informed by electrophysiological data from the hindbrain of rats and from the forebrain of macaques. In multidimensional spaces, generated from patterns of neural activity evoked in the rat's hindbrain by an array of taste stimuli, MSG does indeed lie near the position of NaCl. Yet when sodium transduction is disrupted by the lingual application of amiloride, the impact on the taste response to MSG is minor, and the pattern of activity it elicits is unaltered. Thus, MSG generates a taste quality that transcends saltiness, one that survives even as saltiness is compromised. This conclusion is in accord with the independent transduction mechanisms ascribed to MSG. In the macaque's taste system, MSG is an effective stimulus with a dynamic range of 0.009 to 0.300 M. Approximately one-third of the taste cells at each synaptic relay respond to MSG at 0.1 M. MSG evoked a neural response profile in primary taste cortex that correlated quite well with that elicited by NaCl, in accord with the pronounced salty component humans report for its taste. At succeeding synaptic relays, however, that relationship deteriorated, becoming increasingly distant and labile. At higher-order gustatory levels, MSG evoked a profile that was no more similar to those of the basic stimuli than they are to each other. This implies that MSG warrants independent status as a basic taste stimulus, serving as the prototype for the umami quality.

In: Sensory Neuron

.H. Electrical and transport characteristics of skin of larval Rana catesbeiana Am. J. Physiol 1979 237 R74 R79 Cox T.C. Alvarado R.H. Nystatin studies of the skin of larval Rana catesbeiana Am. J. Physiol 1983 244 R58 R65 Crocker C.E. Stiffler D.F. The effects of amiloride on electrolyte transport

In: Israel Journal of Ecology and Evolution

Na + /H + antiporter in the halotolerant alga Dunaliella salina Plant Physiol. 1991 96 110 115 Katz A. Kleyman T.R. Pick U. Utilization of amiloride analogs for characterization and labeling of the plasma membrane Na + /H + antiporter from Dunaliella salina Biochemistry 1994 33 2389 2393 Katz

In: Israel Journal of Plant Sciences

by NH 4 Cl failed to evoke vesicle release. Collagen and phorbol ester (PMA)-evoked vesiculation was diminished in the presence of 5-( N - ethyl- N -isopropyl amiloride) (EIPA, inhibitor of NHE) or GF 109203X (inhibitor of protein kinase C). Vesicle formation induced by collagen, PMA, and the calcium

In: Haematologia

osmoreceptor with others Von Seckendorff Hoff and Hillyard (1993) and Nagai et al. (1999) in their studies of behav- ioural osmoregulation in the toad, Bufo puncta- tus (Baird and Girard, 1852; Bufonidae), con- cluded, based on trials of solutions of NaCl, urea, and amiloride in various combinations, that

In: Amphibia-Reptilia

Ecological Approach to Visual Perception . Houghton Mifflin, Boston, USA. Halpern, B. P. and Darlington, R. B. (1998). Effects of amiloride on gustatory quality descriptions and temporal patterns produced by NaCl, Chem. Senses 23 , 501–511. Kemps, E. and Tiggemann, M. (2010). A cognitive experimental

In: Seeing and Perceiving