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Gabriel P. Faggioni, Gabriel P. Faggioni, Kelly R. Zamudio, Gabriel P. Faggioni, Kelly R. Zamudio, Franco L. Souza, Gabriel P. Faggioni, Kelly R. Zamudio, Franco L. Souza and Cynthia P.A. Prado

Leptodactylus bufonius (Vizcacheras’ white-lipped frog) and L. chaquensis (Cei’s white-lipped frog) are pond-breeding frogs that inhabit the Chaco and surrounding savanna-like formations in South America. Throughout the Chacoan plain, the combined impacts of livestock and forestry practices have led to a highly fragmented landscape and an impoverished ecological system, threatening local species. We cloned and characterized new microsatellite markers for both species. These markers will be useful for behavioral and conservation genetic studies of populations throughout this threatened habitat.

Valentina Rovelli, Aritz Ruiz-González, Leonardo Vignoli, Daniele Macale, Vincenzo Buono, Francesca Davoli, David R. Vieites, Nadav Pezaro and Ettore Randi

Introduction The rapid progress of Next Generation Sequencing (NGS) platforms has bypassed the need for marker assay development and library construction, allowing a direct comparison of large numbers of genetic sequences to identify DNA polymorphisms in a wide range of species (Narum et al., 2013

Corrette Ploem, Colin Mitchell, Wim van Harten and Sjef Gevers

in a relatively new form, raising questions that have not been sufficiently addressed in the past. This is particularly related to developments in genetics and genomics, especially ‘next generation sequencing’ ( NGS ) and ‘whole genome sequencing’ ( WGS ), technologies currently being introduced in

Patricia Susana Amavet, Eva Carolina Rueda, Pablo Ariel Siroski, Alejandro Larriera and Beatriz Ofelia Saidman

difficult the methodology. We tested these microsatellites in previous works (Amavet et al., 2008 , 2012 ) but of the 13 published markers only 6 could be optimized. It is known that to obtain robust data it is better to analyze many markers as possible (ten at least) and Next Generation Sequencing (NGS

Juan E. Palomares-Rius, Carolina Cantalapiedra-Navarrete and Pablo Castillo

-by-case basis (Wu et al. , 2007; Gutierrez-Gutierrez et al. , 2010; Cantalapiedra et al. , 2013). New technologies Next-generation sequencing (NGS) clearly holds promise for fast and cost effective generation of multilocus sequences for phylogeography and phylogenetics (McCormack et al. , 2013

Junhong Su, Linghao Li, Yueying Wang, Xiao-xia Ma, Zhongren Ma, Maikel P. Peppelenbosch, Qiuwei Pan and Xue Bai

fecal microbiota community of CABBs was analyzed using a next-generation sequencing platform. Analysis of the changes in gut microbiota was performed for both adult and young CABBs. Materials and methods Sample collection All of the bears were housed at a bile farm in Jilin Province, northeast China

Silvia Geser, Laura Kaiser, Valérie Zwahlen and Sylvain Ursenbacher

The Asp viper (Vipera aspis) is a highly endangered species in the Swiss Plateau and in the Jura Mountains of Switzerland. In the past, numerous populations disappeared due to human activities, but currently the trend is reversed and several locations seem favourable for this species. However, it is not known if the remaining populations are still genetically variable and viable. In this study, 12 new microsatellite loci markers were developed for Vipera aspis using Next Generation Sequencing (NGS) techniques (454 sequencing). All markers proved to be polymorphic with 2-9 alleles. When cross-tested with two Eurasian widespread viper species, 8 of the newly developed loci provided reliable amplification for V. berus and 3 loci for V. ammodytes.

Valerie M. Williamson

available soon, leading to an exponential increase in data. Next-generation sequencing techniques promise to add dramatically to this collection of data and, hopefully, to our understanding of the phylum. A chapter describing the voluminous resources available from investigations of the model nematode

Y. S. Hyun, H. Y. Song, J. Woo, B. J. Lim, S. Oh and H. S. An

inferred (Tautz, 1989 ; Rewicz et al., 2015 ; Delghandi et al., 2016 ; Pirog et al., 2016 ). Today, these markers can be developed quickly and at low cost using next-generation sequencing (NGS), even in organisms for which adequate databases are not currently available (Santana et al., 2009 ; Gardner

Sebastian Kiewnick

) until the very recent developments made possible by next-generation-sequencing (NGS) and other state-of-the-art technology, the chapter on the systematics on Pochonia helps the reader to understand fungal systematics, which is in a state of flux, and also demonstrates the largely untapped sources of