Keying into the stream of research on culture and entrepreneurship, this chapter analyses the contribution of culture to entrepreneurship development in Uganda, East Africa. Using existing evidence, this chapter examines the role of different governmental and non-governmental institutions in the development of entrepreneurship in Uganda. Existing evidence reveals that various institutions have put in place policies, initiatives and programmes that facilitate and/or hinder entrepreneurship development in Uganda. This chapter examines these institutions and their roles in detail. Following this discussion, we focus on culture as an institution. We use existing literature, empirical evidence and a case study to analyse and illustrate culture’s facilitative and constraining role in the development of entrepreneurship in Uganda. Our findings reveal that culture facilitates entrepreneurship development by making it possible for individuals to access a social network that may avail various resources for entrepreneurs. Additionally, culturally-induced social obligations contribute to increased costs of raising resources thus hampering entrepreneurship development in Uganda. This chapter offers recommendations for entrepreneurship development in Africa.