I explore the economic, social and cultural constraints of the regional mission of a university located beyond a metropolitan area or urban agglomeration, henceforth referred to as a “peripheral university.” In the first part of the paper, I briefly describe the “third mission” of a university and analyze it within the context of a “peripheral university.” The main constraints on the influences of regional mission and regional development are described. In the second part, I examine one type of a “peripheral university,” namely a cross-border university, on a case study of a consortium of two universities: Viadrina University in Frankfurt am Oder and Collegium Polonicum – a department of Adam Mickiewicz University (CP AMU). I focus on issues like civil mission or problems of the regional contribution of a border university. I also analyze hiddenagenda concerns with respect to the trans-culture added value of the cross-border university. The ensuing analysis is based on interviews made with present and former rectors of those universities.